Phenotypic and molecular dissection of ICRISAT mini core collection of peanut (<i>Arachis hypogaea</i> L.) for high oleic acid

Mukri, Ganapati; Nadaf, H. L.; Bhat, Ramesh; Gowda, M. V. C.; Upadhyaya, Hari D.; Sujay, V.

doi:10.1111/j.1439-0523.2012.01970.x

Cited by 28 publications

(26 citation statements)

References 28 publications

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“…Similarly, in US mini-core collections, 44% (Wang et al, 2011b), 31.6% (Chu et al, 2007) and 41% (Wang et al, 2013b); while in ICRISAT mini-core collections, 49.5% (Mukri et al, 2012) ahFAD2A mutant allele was reported. But, none found having ahFAD2B mutant allele.…”

Section: Discussionmentioning

confidence: 85%

“…Similarly, in ahFAD2A mutant germplasm lines, the range of mean O/L ratio recorded was 1.11–6.93 with an average of 2.43, whereas, in the non-mutant accessions it was 1.26 with a range of 0.77–2.55 (Mukri et al, 2012). Majority of the ahFAD2A mutant genotypes were found having O/L ratio above 1.5, while non-mutant lines had less than that, with some exceptions (Table 1).…”

Section: Discussionmentioning

confidence: 94%

“…These tools have been successfully utilized for the screening of peanut germplasm (Chu et al, 2007; Wang et al, 2011a,b, 2013b; Mukri et al, 2012). However, no such efforts have been reported for the Indian peanut cultivars and advanced breeding lines, which are being used in improving the O/L ratio of the Indian genotypes by the breeders.…”

Section: Introductionmentioning

confidence: 99%

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Insights into the Indian Peanut Genotypes for ahFAD2 Gene Polymorphism Regulating Its Oleic and Linoleic Acid Fluxes

et al. 2016

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In peanut (Arachis hypogaea L.), the customization of fatty acid profile is an evolving area to fulfill the nutritional needs in the modern market. A total of 174 peanut genotypes, including 167 Indian cultivars, 6 advanced breeding lines and “SunOleic95R”—a double mutant line, were investigated using AS-PCRs, CAPS and gene sequencing for the ahFAD2 allele polymorphism, along with its fatty acid compositions. Of these, 80 genotypes were found having substitution (448G>A) mutation only in ahFAD2A gene, while none recorded 1-bp insertion (441_442insA) mutation in ahFAD2B gene. Moreover, 22 wild peanut accessions found lacking both the mutations. Among botanical types, the ahFAD2A mutation was more frequent in ssp. hypogaea (89%) than in ssp. fastigiata (17%). This single allele mutation, found affecting not only oleic to linoleic acid fluxes, but also the composition of other fatty acids in the genotypes studied. Repeated use of a few selected genotypes in the Indian varietal development programs were also eminently reflected in its ahFAD2 allele polymorphism. Absence of known mutations in the wild-relatives indicated the possible origin of these mutations, after the allotetraploidization of cultivated peanut. The SNP analysis of both ahFAD2A and ahFAD2B genes, revealed haplotype diversity of 1.05% and 0.95%, while Ka/Ks ratio of 0.36 and 0.39, respectively, indicating strong purifying selection pressure on these genes. Cluster analysis, using ahFAD2 gene SNPs, showed presence of both mutant and non-mutant genotypes in the same cluster, which might be due the presence of ahFAD2 gene families. This investigation provided insights into the large number of Indian peanut genotypes, covering various aspects related to O/L flux regulation and ahFAD2 gene polymorphism.

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Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 94%

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Insights into the Indian Peanut Genotypes for ahFAD2 Gene Polymorphism Regulating Its Oleic and Linoleic Acid Fluxes

et al. 2016

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“…Several efforts have been made previously for QTL discovery using sparse genetic maps constructed using SSRs (Huang et al 2015;Varshney et al 2009;Wang et al 2012Wang et al , 2015. Since the genetic base of cultivated peanut is narrow (Belamkar et al 2011;Mukri et al 2012), available SSR markers reported in earlier studies are limited for developing saturated genetic maps. However, single nucleotide polymorphisms (SNPs) are abundant and widely distributed across the genome.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

High-resolution mapping of a major and consensus quantitative trait locus for oil content to a ~ 0.8-Mb region on chromosome A08 in peanut (Arachis hypogaea L.)

et al. 2019

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Key message ddRAD-seq-based high-density genetic map comprising 2595 loci identified a major and consensus QTL with a linked marker in a 0.8-Mb physical interval for oil content in peanut. Abstract Enhancing oil content is an important breeding objective in peanut. High-resolution mapping of quantitative trait loci (QTLs) with linked markers could facilitate marker-assisted selection in breeding for target traits. In the present study, a recombined inbred line population (Xuhua 13 × Zhonghua 6) was used to construct a genetic map based on double-digest restriction-site-associated DNA sequencing (ddRAD-seq). The resulting high-density genetic map contained 2595 loci, and spanned a length of 2465.62 cM, with an average distance of 0.95 cM/locus. Seven QTLs for oil content were identified on five linkage groups, including the major and stable QTL qOCA08.1 on chromosome A08 with 10.14–27.19% phenotypic variation explained. The physical interval of qOCA08.1 was further delimited to a ~ 0.8-Mb genomic region where two genes affecting oil synthesis had been annotated. The marker SNPOCA08 was developed targeting the SNP loci associated with oil content and validated in peanut cultivars with diverse oil contents. The major and stable QTL identified in the present study could be further dissected for gene discovery. Furthermore, the tightly linked marker for oil content would be useful in marker-assisted breeding in peanut.

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“…Low O/L hence indicates low oxidative stability (Yanishlieva and Marinova, 2001). On the other hand a high ratio is desirable to enhance oxidative stability and nutritional value (Flagella, Rotunno, Tarantino, Di Caterina, & De Caro, 2002;Mukri et al, 2012). Oyster nut oil had low O/L value of <1 (Table 4).…”

Section: Lipid Health Indices For Oyster Nut Oilmentioning

confidence: 99%

Fatty Acid Composition of Oil from Groundnuts and Oyster Nuts Grown in Uganda

Musalima¹,

Ogwok²,

Mugampoza³

2019

JFR

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This study determined the fatty acid (FA) composition of oil from groundnuts and oyster nuts in Uganda. The FA composition was determined by Gas Chromatography/Mass Spectrometry with FID. Oil from groundnuts contained 39.71 to 55.89% oleic, 20.21 to 35.59% linoleic and 11.91 to 17.16% palmitic acids. Oil from Serenut cultivars contained cis 11-eicosenoic acid (C20.1), cis 11, 14 eicosadienoic acid (C20.2) and cis 11, 14, 17 eicosatrienoic acid (C20.3ω3) which were not detected in traditional cultivars. Oyster nut oil was high in linoleic acid at 41.02 to 44.86% and palmitic acid at 33.58 to 38.11% while oleic acid was low with amounts <10%. The polyunsaturated to saturated FA ratios of oil from groundnuts and oyster nuts were >0.45, the minimum recommended by FAO. The atherogenic (AI) and thrombogenic indices (TI) of <1 and the hypocholesterolemic to hypercholesterolemic index (h/H) of >4 in groundnut oil were favorable for cardiovascular health. Indices for oyster nut oil were ≤1for AI and >1for TI. The h/H was low compared to that of groundnut oil. Results showed little distinction between the FA compositions of oil from traditional and improved groundnuts suggesting that breeding may not have significant effect on major FAs. Oyster nut oil contained saturated and unsaturated FA in a ratio of 1:1. The lipid health indices for groundnut oil were within recommendations while those of oyster nuts were less desirable. Oyster nut oil should therefore be consumed with moderation.

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Phenotypic and molecular dissection of ICRISAT mini core collection of peanut (Arachis hypogaea L.) for high oleic acid

Cited by 28 publications

References 28 publications

Insights into the Indian Peanut Genotypes for ahFAD2 Gene Polymorphism Regulating Its Oleic and Linoleic Acid Fluxes

Insights into the Indian Peanut Genotypes for ahFAD2 Gene Polymorphism Regulating Its Oleic and Linoleic Acid Fluxes

High-resolution mapping of a major and consensus quantitative trait locus for oil content to a ~ 0.8-Mb region on chromosome A08 in peanut (Arachis hypogaea L.)

Fatty Acid Composition of Oil from Groundnuts and Oyster Nuts Grown in Uganda

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