Phenotypic and molecular characterization of new interspecific Japanese plum × apricot hybrids (plumcots)

Nicolás-Almansa, María; Ruiz, David; Salazar, J.; Guevara, Alfonso; Cos, J.; Martínez‐Gómez, Pedro; Rubio, M.

doi:10.1016/j.scienta.2023.112131

Cited by 3 publications

(1 citation statement)

References 66 publications

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“…The improved consensus primer pair flanking the first intron of the S-RNase gene, namely PaConsI-F/R2, was suggested for high throughput S-allele genotyping (Sonneveld et al, 2006). This system has become well-established and has been used extensively for S-allele genotyping of sweet cherry (Vaughan et al, 2008;Ganopoulos et al, 2012;Sharma et al, 2016;Cachi et al, 2017;Marchese et al, 2017;Azizi-Gannouni et al, 2018) and has also be transferred to other Prunus species (Halaśz et al, 2021a, Halaśz et al, 2021bNicolaś-Almansa et al, 2023). It further served as a basis for the further development of the genotyping method, such as the one-tube reaction assay described by Cmejlova et al (2023).…”

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confidence: 99%

Synergistic approach of PCR-based fragment length analysis and amplicon deep sequencing reveals rich diversity of S-alleles in sweet cherries from the Caucasian region of origin

Schröpfer,

Schuster,

Quero-Garcia

et al. 2024

Front. Plant Sci.

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IntroductionThe self-incompatibility system in sweet cherry (Prunus avium L.) prevents fertilization with own or genetically related pollen, and is genetically determined by the multi-allelic S-locus. Therefore, determining S-alleles is crucial for plant breeding and fruit production, as it enables the selection of compatible combinations of S-genotypes for successful pollination.MethodsIn this study, S-alleles were identified in a total of 260 genotypes from the Caucasian region, the species’ center of origin. S-allele genotyping was conducted using PCR fragment length analysis with the standard marker PaConsI-F/R2 and reference genotypes, complemented by sequence analysis through amplicon deep sequencing.Results and discussionThe genotypes collected from Azerbaijan and Turkey exhibit a high allelic richness at the S-locus, particularly compared to modern sweet cherry cultivars worldwide. Nine previously undescribed S-alleles were identified and designated as S45, S46, S47, S48, S49, S50, S51, S52 and S53. Given the expected high diversity for other traits, this plant material represents a valuable resource for further breeding research and introgression of new traits in future breeding programs. Furthermore, our results underscore that fragment length alone may not be sufficient for unambiguous assignment of S-alleles due to minimal length differences between different alleles. To address this issue, an S-allele reference ladder was developed using the rich diversity for precise assignment of the S-alleles. This tool can be applied in future experiments as a robust and cost-effective method for accurate S-genotyping across different runs and laboratories. Additionally, several selected S-genotypes were planted in a trial field and will be maintained as an S-allele reference collection.

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Section: Introductionmentioning

confidence: 99%

Synergistic approach of PCR-based fragment length analysis and amplicon deep sequencing reveals rich diversity of S-alleles in sweet cherries from the Caucasian region of origin

Schröpfer,

Schuster,

Quero-Garcia

et al. 2024

Front. Plant Sci.

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Determining of thermal time and base temperature during the reproductive phase of the Japanese plum in the tropical Andes

Orjuela-Angulo,

Balaguera-Lopez,

Fischer

2024

Rev. Colomb. Cienc. Hortic

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Climate factors affect the phenological behavior of Japanese plum (Prunus salicina Lindl.) cultivation in tropical elevation. Thermal time is more precise than chronological time for the cultivation labor program and for the estimation of crop phenological events. The objective of this study was to determine the base temperature (Tb) and thermal time for the development of the 'Horvin' plum fruit, estimating the Tb for the reproductive phenological phase of the plum. Twenty trees, consisting of ten trees per rowat two different elevations of 2,195 and 2,567 m a.s.l. (meters above sea level), in the municipality of Nuevo Colon (Colombia), were marked by locality. The appearance of each phenological stage was recorded from floral bud to harvest, estimating Tb with the minimum coefficient method of variation in function of growing degree day (GDD). The results show Tb for the phenological stages floral-anthesis with 2.84°C; anthesis-fruits set, 3.05 °C, and fruit set-harvest, 1.76°C. Therefore, this variety should be grown where there is a minimum temperature of 3.05°C during the reproductive phase. In addition, the results show that an average of 1,243.17 and 1,172.12 GDD is required to go from fruit set to fruit harvest at elevations 2,195 and 2,567 m a.s.l., respectively.

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Phenotypic characteristics of black apricot Prunus × dasycarpa cultivar ‘Shlor-Tsiran’ in Armenia

Akopian,

Navasardyan,

Rudov

et al. 2024

Folia Horticulturae

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‘Shlor-Tsiran’ (literally called ‘Plum-Apricot’) is an old black apricot cultivar, which is traditionally cultivated in Armenia. Taxonomically, it belongs to Prunus × dasycarpa Ehrh., which is a spontaneous hybrid of apricot and cherry plum (P. armeniaca L. × P. cerasifera Ehrh.). Currently, ‘Shlor-Tsiran’ is considered to be very rare in Armenia and it occurs as single trees in old home orchards of the Ararat Valley. It is characterised by high frost and disease resistance, late flowering and fruiting compared with other cultivated apricots, and is of interest for the development of apricot cultivars displaying delayed fruit ripening and enhanced frost resistance. This study aims to phenotype the cv. ‘Shlor-Tsiran’ taking into consideration 67 quantitative and qualitative characteristics of vegetative and generative organs, growth and development. Morphological description of the cv. ‘Shlor-Tsiran’, comparing with its parent species, P. armeniaca and P. cerasifera, was performed. Data on fertility and viability of pollen grains, biochemical and nutritional properties of fruits and tree productivity are provided. The data obtained should contribute to a wider use and conservation of the black apricot cv. ‘Shlor-Tsiran’ gene pool, which can be used for breeding purposes as well. The article is illustrated with tables and original photographs.

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Phenotypic and molecular characterization of new interspecific Japanese plum × apricot hybrids (plumcots)

Cited by 3 publications

References 66 publications

Synergistic approach of PCR-based fragment length analysis and amplicon deep sequencing reveals rich diversity of S-alleles in sweet cherries from the Caucasian region of origin

Synergistic approach of PCR-based fragment length analysis and amplicon deep sequencing reveals rich diversity of S-alleles in sweet cherries from the Caucasian region of origin

Determining of thermal time and base temperature during the reproductive phase of the Japanese plum in the tropical Andes

Phenotypic characteristics of black apricot Prunus × dasycarpa cultivar ‘Shlor-Tsiran’ in Armenia

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