Phenotypic and molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) circulating into environments in Ouagadougou, Burkina Faso.

Abstract

Meticillin-resistant Staphylococcus aureus (MRSA) diagnostic techniques are not very easy in rattan practices in Burkina Faso and West African. This is main difficulty of good MRSA surveillance in this region. Thus, to consolidate MRSA data in locality that, 33 strains of S. aureus were tested for susceptibility to cefoxitin according using standard method. In addition, the genetic determinants including mecA gene and SCCmec cassettes were searched using conventional PCR technic to same strains. These protocols made it possible to obtain prevalence’s of 42.42% (14/33) and 18.18% (6/33) respectively of S. aureus resistant to cefoxitin and positive in Chrom MRSA ID® agar medium. From 33 S. aureus studied, 6 strains (18.18%) and 7 strains (21.21%) all of human origin were respectively positive for mecA resistance gene and SCCmec IVa cassette. This study brought circulating MRSA in the city of Ouagadougou. These MRSA harbor mecA resistance genes and SCCmec IVa cassettes.