Phenotypic and molecular characterization of diazotrophic bacteria associated with sugarcane in Bangladesh

Isolation and Characterization of Plant Growth-Promotion Diazotrophic Endophytic Bacteria Associated to Sugarcane (Saccharum officinarum L.) Grown in Paraíba, Brazil

“…In this study, 56 bacterial isolates were obtained from sugarcane tissues, being 21 and 35 isolates found in leaves and stems, respectively. Interestingly, we found a higher number of endophytic bacterial isolates in sugarcane tissues than those found in previous studies with different crops, such as sorghum [32] and sugarcane [33]. For instance, Patel and Archana [34] isolated, through NFb broth, 31 endophytic diazotrophic bacteria in leaves, stems, and roots from Poaceae plants.…”

“…Endophytic bacteria from sugarcane leaves and stems showed fast growth and were mainly Grampositive (Figure 1F and 1G). In contrast, Magnani and coauthors [35] and Alam and coauthors [33] reported that most of the endophytic bacteria from sugarcane are Gram-negative. On the other hand, the majority of bacterial endophytes from different plant tissues of rice were Gram-positive [37].…”

“…As a primary auxin in higher plants, IAA has marked effects on plant development. Previous studies on several plant species, such as chickpea, maize, rice, sorghum, sugarcane, tomato, and wheat, have reported bacteria releasing IAA and promoting plant growth [9,33,34,40].…”

“…For instance, Patel and Archana [34] isolated, through NFb broth, 31 endophytic diazotrophic bacteria in leaves, stems, and roots from Poaceae plants. By comparison, Leite and coauthors [11] found 24 endophytic diazotrophic bacteria in stems and roots of sugarcane by using LGI-P broth, while Alam and coauthors [33] found 21 bacterial isolates in roots and stems of sugarcane by using LGI broth. A total of 30, 18 and 8 bacterial isolates grown, respectively, in NFb, JNFb and LGI-P broths.…”

“…Methylobacterium and Herbaspirillum are genera of diazotrophs belonging to the phylum Proteobacteria. This phylum includes bacterial endophytes that perform nitrogen-fixation in different tissues of monocots, including rice, maize, sorghum, and sugarcane [8,33,34].…”