Phenotypic and Molecular Characterization of Plasmid Mediated AmpC among Clinical Isolates of Klebsiella pneumoniae Isolated from Different Hospitals in Tehran

Azimi, Leila

doi:10.7860/jcdr/2014/11037.5797

Cited by 8 publications

(5 citation statements)

References 6 publications

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“…Our analysis revealed the high prevalence of AmpC beta-lactamase among clinical isolates of K. pneumoniae isolated from burn patients. According to Azimi et al's study during 2013 in Iran, the prevalence of AmpC beta-lactamase among clinical isolates of K. pneumoniae was reported 1.6% [17]. Our data showed the sharp rise of AmpC beta-lactamase incidence over the past 3 years.…”

Section: Discussionsupporting

confidence: 48%

First report of coexistence of AmpC beta-lactamase genes in Klebsiella pneumoniae strains isolated from burn patients

Ghanavati

Darban-Sarokhalil

Navab-Moghadam

et al. 2017

AMicr

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Klebsiella spp. are among the most frequently isolated bacteria from burn wounds. These organisms are among the most important opportunistic pathogens, causing hospital-acquired and healthcare-associated infections worldwide. Limited information is available about prevalence of AmpC-producing Klebsiella pneumoniae from burn patients. Therefore, the aim of this study was to determine the characterization of AmpC beta-lactamase among K. pneumoniae isolated from burn patients. Samples were collected from wound specimens of patients with burn injury from a burn hospital in Tehran during 18 months (March 2015 to August 2016). For phenotypic detection of AmpC beta-lactamase, disk diffusion method with cefoxitin was used for screening, AmpC disk test and boronic acid inhibitor-based method were used as confirmatory tests. Polymerase chain reaction (PCR) was performed to screen all isolates with AmpC genes including ACCM, DHAM, EBCM, FOXM, MOXM, and CITM. Finally, PCR products were validated using sequencing. During this study, 102 isolates of K. pneumoniae were collected. Among these isolates, 52.9% suspected as AmpC producer by disk agar diffusion cefoxitin screening method. By confirmatory phenotypic methods, 19.6% of isolates considered as AmpC producer. Molecular analysis revealed 43.1% of cefoxitin-resistant isolates harbored at least one of the AmpC genes including CITM (22.5%), EBCM (21.5%), DHAM (7.8%), and FOXM (0.98%). In addition, 5.8% of isolates harbored two AmpC genes and 2.9% harbored three AmpC genes. In conclusion, K. pneumoniae is becoming a serious problem in burn patients. Accurate and precise methods and guidelines should be designed for detection of antibiotic-resistant mechanisms. Our data showed the high rate of AmpC beta-lactamase among K. pneumoniae isolated from burn patients, which limit the treatment options. Therefore, the results of this study can provide evidence to help for appropriate treatment of burn patients.

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Section: Discussionsupporting

confidence: 48%

First report of coexistence of AmpC beta-lactamase genes in Klebsiella pneumoniae strains isolated from burn patients

Ghanavati

Darban-Sarokhalil

Navab-Moghadam

et al. 2017

AMicr

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“…Enterobacteriaceae is a family of Gram-negative bacteria that are associated with a wide variety of community and nosocomial infections [ 1 , 2 ]. Antibiotic resistance may be chromosomal dependent or plasmid mediated so it is easily transferred between bacteria [ [1] , [2] , [3] , [4] ]. Carbapenems have a broad spectrum of activity against Gram-negative bacteria and are used as one of the last lines of antibiotic therapy, especially in cases of infection with extended-spectrum β-lactamase–producing organisms [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Multicentre study of the main carbapenem resistance mechanisms in important members of the Enterobacteriaceae family in Iran

Armin

Fallah

Karimi

et al. 2021

New Microbes and New Infections

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Resistance to carbapenems has been increasingly reported from the Enterobacteriaceae family, with different mechanisms in different geographic parts of the world. This study investigated the mechanisms of carbapenem resistance in Escherichia coli, Klebsiella pneumoniae and Enterobacter spp. carried out as a multicentre study ( n = 10). All third-generation cephalosporin-resistant E. coli, K. pneumoniae and Enterobacter spp. that had been recovered from the selected provinces were included. Modified Hodge test and Carba NP test were done as a phenotypical method for detection of carbapenemase; the most common carbapenemase was detected by PCR. We evaluated the presence of an active efflux pump by using cyanide 3-chlorophenylhydrazone. Overexpression of AcrA/B and presence of OqxAB was detected by real-time PCR and conventional PCR respectively. Microorganisms in this study included 58 E. coli , 95 K. pneumoniae and 60 Enterobacter spp. Modified Hodge test showed a sensitivity of 41% and a specificity of 83%, and the Carba NP test showed a sensitivity of 26% and a specificity of 92% for detection of carbapenemase. OXA-48 was the most frequently detected carbapenemase, followed by NDM-1. Thirty-nine percent and 27% of positive cyanide 3-chlorophenylhydrazone test organisms included active AcrA/B and OqxAB efflux pumps respectively. The result showed the Carba NP test was more specific than MHT. Data confirmed the involvement of AcrA/B and OqxAB efflux pump as a carbapenem resistance mechanism in selected bacteria. Similar to other reports from the Middle East, we found OXA-48 and NDM-1 to be the most frequent carbapenemase.

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“…(13,6%) foram positivos para AmpC. 30 No presente estudo, somadas as vezes em que AmpC foi detectada pelo teste D e de sinergismo com ácido borônico e cloxacilina, foi encontrado 32,3% de positividade.…”

Section: Discussionunclassified

Produção de β-lactamases em isolados clínicos de Enterobacteriaceae resistentes aos carbapenêmicos / β-lactamases em Enterobacteriaceae production of β-lactamases in clinical isolates of carbapenem-resistant Enterobacteriaceae

Dorigoni¹,

Freitas²,

Queiroz³

et al. 2021

BJDV

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RESUMOAvaliar isolados clínicos de Enterobacteriaceae, quanto à produção de β-lactamases e o perfil de multirresistência. As amostras foram isoladas de um hospital mineiro, de 2014 a 2017 e avaliadas por meio dos antibiogramas e testes fenotípicos de duplo disco para βlactamases, teste D e sinergismo. Foram recuperadas 93 amostras, a maioria de enfermarias, isoladas de cultura de vigilância e sangue. A bactéria mais presente foi Klebsiella pneumoniae e os pacientes acometidos tinham distribuição diferente, mais homens em UTIs, idade entre 41 e 80 anos e maior ocorrência no ano de 2017. Os mecanismos de resistência mais frequentes foram produção de KPC e ESBL, porém com importante participação das MBL. A endemicidade destes microrganismos implica em

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Phenotypic and Molecular Characterization of Plasmid Mediated AmpC among Clinical Isolates of Klebsiella pneumoniae Isolated from Different Hospitals in Tehran

Cited by 8 publications

References 6 publications

First report of coexistence of AmpC beta-lactamase genes in Klebsiella pneumoniae strains isolated from burn patients

First report of coexistence of AmpC beta-lactamase genes in Klebsiella pneumoniae strains isolated from burn patients

Multicentre study of the main carbapenem resistance mechanisms in important members of the Enterobacteriaceae family in Iran

Produção de β-lactamases em isolados clínicos de Enterobacteriaceae resistentes aos carbapenêmicos / β-lactamases em Enterobacteriaceae production of β-lactamases in clinical isolates of carbapenem-resistant Enterobacteriaceae

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