Phenotypic and Molecular Characterization of Enteroaggregative &lt;i&gt;Escherichia coli&lt;/i&gt; Isolated in Kawasaki, Japan

Kubomura, Akiko; Misaki, Takako; Homma, Sachiko; Matsuo, Chiaki; Okabe, Nobuhiko

doi:10.7883/yoken.jjid.2016.387

Cited by 7 publications

(4 citation statements)

References 35 publications

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“…E. coli isolates were confirmed as EAEC by the amplification of aggR gene as previously described. 12 Subsequently, the presence of five virulence factors were determined by assessing the presence of pCVD, aggA, ast, aap , and aafA genes. 13 Moreover, the presence of ESBL resistance genes, bla TEM , bla P ER , bla CTX-M , and bla SHV were determined by specific primers.…”

Section: Methodsmentioning

confidence: 99%

Antibiotic resistance pattern and molecular characterization of extended-spectrum β-lactamase producing enteroaggregative <em>Escherichia coli</em> isolates in children from southwest Iran

Amin¹,

Sirous²,

Javaherizadeh³

et al. 2018

IDR

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IntroductionEnteroaggregative Escherichia coli (EAEC) has been implicated as an emerging cause of traveler’s diarrhea, persistent diarrhea among children, and immunocompromised patients. The present study aimed to investigate the prevalence of antibiotic resistance, extendedspectrum β-lactamase (ESBL) production, and virulence factors of EAEC isolates obtained from Iranian children suffered from diarrhea.Materials and methodsIn this cross-sectional study, from March 2015 to February 2016, 32 EAEC isolates were collected from fecal samples of children aged <12 years with diarrhea in southwest of Iran. All EAEC isolates identified using phenotypic and molecular methods and the cell line adhesion assay. Antimicrobial susceptibility testing was determined using disk diffusion method. The presence of virulence factors and ESBL resistance genes were determined by polymerase chain reaction.ResultsOverall, 28.1% (9/32) of the isolates were positive for at least one of virulence genes. The most frequent gene was aap with a frequency of 96.9%. Neither aafA nor aggA gene was detected among all of the EAEC isolates. Antimicrobial susceptibility testing revealed the highest resistance rate to ampicillin (100%) and co-trimoxazole (100%), followed by ceftriaxone (81.3%). Further analysis revealed that the rate of ESBLs-producing isolates was 71.9% (23/32). Polymerase chain reaction screening revealed that 87.5% and 65.5% of EAEC isolates were positive for blaTEM and blaCTX-M genes, respectively, and 17 (53.1%) of isolates contained both blaTEM and blaCTX-M genes.ConclusionThe high detection rate of ESBL-producing EAEC isolates accompanied with virulence genes highlights a need to restrict infection control policies in order to prevent further dissemination of the resistant and virulent EAEC strains.

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Section: Methodsmentioning

confidence: 99%

Antibiotic resistance pattern and molecular characterization of extended-spectrum β-lactamase producing enteroaggregative <em>Escherichia coli</em> isolates in children from southwest Iran

Amin¹,

Sirous²,

Javaherizadeh³

et al. 2018

IDR

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“…All DEC isolates, except for 51 of the STEC isolates, were obtained from stool samples collected from asymptomatic carriers and patients with gastrointestinal symptoms at the Kawasaki City Institute for Public Health, Japan, from 2012 to 2014. e remaining 51 STEC isolates were collected from outpatients at several hospitals in Kawasaki between April 2012 and December 2014 ( Table 1). e 40 EAEC isolates were also examined in our previous study of antimicrobial resistance patterns [13].…”

Section: Bacterial Strainsmentioning

confidence: 99%

Truncated Class 1 Integron Gene Cassette Arrays Contribute to Antimicrobial Resistance of Diarrheagenic Escherichia coli

Kubomura¹,

Sekizuka

Onozuka

et al. 2020

BioMed Research International

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Class 1 integrons (c1-integrons) are associated with multidrug resistance in diarrheagenic Escherichia coli (DEC). However, little is known about gene cassettes located within these c1-integrons, particularly truncated c1-integrons, in DEC strains. erefore, the aims of the present study were to reveal the relationship between antimicrobial resistance and the presence of truncated c1-integrons in DEC isolates derived from human stool samples in Japan. A total of 162 human stool-derived DEC isolates from Japan were examined by antimicrobial susceptibility testing, PCR-based gene detection, and next-generation sequencing analyses. Results showed that 44.4% (12/27) of c1-integrons identified in the DEC isolates harbored only intI1 (an element of c1-integrons) and were truncated by IS26, Tn3, or IS1-group insertion sequences. No difference in the frequency of antimicrobial resistance was recorded between intact and truncated c1-integron-positive DEC isolates. Isolates containing intact/truncated c1-integrons, particularly enteroaggregative E. coli isolates, were resistant to a greater number of antimicrobials than isolates without c1-integrons. aadA and dfrA were the most prevalent antimicrobial resistance genes in the intact/ truncated c1-integrons examined in this study. erefore, gene cassettes located within these intact/truncated c1-integrons may only play a limited role in conferring antimicrobial resistance among DEC. However, DEC harboring truncated c1integrons may be resistant to a greater number of antimicrobials than c1-integron-negative DEC, similar to strains harboring intact c1-integrons.

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“…The strain was phylogenetically closely related to some strains of EAEC (042) and UPEC (UMN026). Previously, EAEC OgGp9:H18 strains harbouring aggR were also isolated from patients with gastrointestinal symptoms [ 31 ]. However, the E. coli OUT (OgGp9):H18 strains in our study did not possess typical EAEC virulence factors such as aggR , suggesting the presence of other atypical virulence factors in the strains.…”

Section: Discussionmentioning

confidence: 99%

Atypical diarrhoeagenic Escherichia coli in milk related to a large foodborne outbreak

Hirose,

Ohya,

Yoshinari

et al. 2023

Epidemiol. Infect.

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A foodborne outbreak related to milk cartons served in school lunches occurred in June 2021, which involved more than 1,800 cases from 25 schools. The major symptoms were abdominal pain, diarrhoea, vomiting, and fever. Although major foodborne toxins and pathogens were not detected, a specific Escherichia coli strain, serotype OUT (OgGp9):H18, was predominantly isolated from milk samples related to the outbreak and most patients tested. The strains from milk and patient stool samples were identified as the same clone by core genome multilocus sequence typing and single-nucleotide polymorphism analysis. The strain was detected in milk samples served for two days related to the foodborne outbreak at a rate of 69.6% and levels of less than ten most probable number/100 mL but not on days unrelated to the outbreak. The acid tolerance of the strain for survival in the stomach was similar to that of enterohaemorrhagic E. coli O157:H7, and the same inserts in the chu gene cluster in the acid fitness island were genetically revealed. The pathogenicity of the strain was not clear; however, it was indicated that the causative pathogen was atypical diarrhoeagenic E. coli OUT (OgGp9):H18.

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Phenotypic and Molecular Characterization of Enteroaggregative <i>Escherichia coli</i> Isolated in Kawasaki, Japan

Cited by 7 publications

References 35 publications

Antibiotic resistance pattern and molecular characterization of extended-spectrum β-lactamase producing enteroaggregative <em>Escherichia coli</em> isolates in children from southwest Iran

Antibiotic resistance pattern and molecular characterization of extended-spectrum β-lactamase producing enteroaggregative <em>Escherichia coli</em> isolates in children from southwest Iran

Truncated Class 1 Integron Gene Cassette Arrays Contribute to Antimicrobial Resistance of Diarrheagenic Escherichia coli

Atypical diarrhoeagenic Escherichia coli in milk related to a large foodborne outbreak

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Phenotypic and Molecular Characterization of Enteroaggregative <i>Escherichia coli</i> Isolated in Kawasaki, Japan

Cited by 7 publications

References 35 publications

Antibiotic resistance pattern and molecular characterization of extended-spectrum &beta;-lactamase producing enteroaggregative <em>Escherichia coli</em> isolates in children from southwest Iran

Antibiotic resistance pattern and molecular characterization of extended-spectrum &beta;-lactamase producing enteroaggregative <em>Escherichia coli</em> isolates in children from southwest Iran

Truncated Class 1 Integron Gene Cassette Arrays Contribute to Antimicrobial Resistance of Diarrheagenic Escherichia coli

Atypical diarrhoeagenic Escherichia coli in milk related to a large foodborne outbreak

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Antibiotic resistance pattern and molecular characterization of extended-spectrum β-lactamase producing enteroaggregative <em>Escherichia coli</em> isolates in children from southwest Iran

Antibiotic resistance pattern and molecular characterization of extended-spectrum β-lactamase producing enteroaggregative <em>Escherichia coli</em> isolates in children from southwest Iran