Phenotypic and molecular characterization and complete genome sequence of a Corynebacterium diphtheriae strain isolated from cutaneous infection in an immunized individual

Araújo, Max Roberto Batista; Ramos, Juliana Nunes; de Oliveira Sant’Anna, Lincoln; Bokermann, Sérgio; Santos, Marlon Benedito Nascimento; Mattos-Guaraldi, Ana Luiza; Azevedo, Vasco; Prates, Fernanda Diniz; Rodrigues, Diego Lucas Neres; Aburjaile, Flávia Figueira; Sacchi, Claudio Tavares; Campos, Karoline Rodrigues; Alvim, Luige Biciati; Vieira, Verônica Viana; Camargo, Carlos Henrique; dos Santos, Louisy Sanches

doi:10.1007/s42770-023-01086-z

Cited by 2 publications

(1 citation statement)

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“…Despite advances resulting from universal vaccination programs with the diphtheria toxoid, which drastically reduced the number of cases worldwide, diphtheria is still reported even in immunized individuals [2,3]. In recent years, the number of cases and outbreaks of diphtheria have been reported in several countries, including Brazil, where diphtheria is endemic, as well as Austria, Belgium, France, Norway, Switzerland, and the United Kingdom [2][3][4][5][6].…”

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confidence: 99%

First Pangenome of Corynebacterium rouxii, a Potentially Toxigenic Species of Corynebacterium diphtheriae Complex

Prates,

Araújo,

Sousa

et al. 2024

Bacteria

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Corynebacterium rouxii is one of the recently described species of the Corynebacterium diphtheriae complex. As this species can potentially infect different hosts and harbor the tox gene, producing diphtheria toxin, we present its first pangenomic analysis in this work. A total of fifteen genomes deposited in online databases were included. After confirming the taxonomic position of the isolates by genomic taxonomy, the genomes were submitted to genomic plasticity, gene synteny, and pangenome prediction analyses. In addition, virulence and antimicrobial resistance genes were investigated. Finally, epidemiological data were obtained through molecular typing, clustering, and phylogenetic analysis. Our data demonstrated genetic diversity within the species with low synteny. However, the gene content is extensively conserved, and the pangenome is composed of 2606 gene families, of which 1916 are in the core genome and 80 are related to unique genes. Prophages, insertion sequences, and genomic islands were found. A type I-E CRISPR-Cas system was also detected. Besides the tox gene, determinants involved in adhesion and iron acquisition and two putative antimicrobial resistance genes were predicted. These findings provide valuable insight about this species’ pathogenicity, evolution, and diversity. In the future, our data can contribute to different areas, including vaccinology and epidemiology.

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