Phenotypic and molecular characteristics of methicillin-resistant and methicillin-susceptible &lt;em&gt;Staphylococcus aureus&lt;/em&gt; isolated from pigs: implication for livestock-association markers and vaccine strategies

Guo, Dan; Liu, Yangqun; Han, Changlin; Chen, Zhiyao; Ye, Xiaohua

doi:10.2147/idr.s173624

Cited by 32 publications

(31 citation statements)

References 57 publications

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“…As in our study, in other studies reporting the presence of CC97 MRSA, the isolates are frequently multidrug resistant with resistance only to β-lactams and tetracyclines but also to macrolides, lincosamides and aminoglycosides [17,31]. Although the relationship between antibiotic resistance profiles and clonal complexes is still not clear, some studies suggest that different LA-MRSA clones may present specific resistance patterns [42]. Macrolide-lincosamide-streptogramin (MLS) resistance occurs by the cross-resistance among these antibiotics, and bacteria with resistance to MLS were previously reported to be transmitted from animals to humans through the food chain [43].…”

Section: Characterization Of Cc97 Mrsa Isolatessupporting

confidence: 66%

Livestock-Associated Methicillin-Resistant Staphylococcus aureus (MRSA) in Purulent Subcutaneous Lesions of Farm Rabbits

Silva

Sousa

Gómez

et al. 2020

Foods

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Methicillin-resistant Staphylococcus aureus (MRSA) are one of the main pathogens associated with purulent infections. MRSA clonal complex 97 (CC97) has been identified in a wide diversity of livestock animals. Therefore, we aimed to investigate the antibiotic resistance profiles of MRSA strains isolated from purulent lesions of food-producing rabbits. Samples from purulent lesions of 66 rabbits were collected in a slaughterhouse in Portugal. Samples were seeded onto ORSAB plates with 2 mg/L of oxacillin for MRSA isolation. Susceptibility to antibiotics was tested by the disk diffusion method against 14 antimicrobial agents. The presence of resistance genes, virulence factors and the immune evasion cluster (IEC) system was studied by polymerase chain reaction. All isolates were characterized by multilocus sequence typing (MLST), agr and spa typing. From the 66 samples analyzed, 16 (24.2%) MRSA were detected. All strains were classified as multidrug-resistant as they were resistant to at least three classes of antibiotics. All isolates showed resistance to penicillin, erythromycin and clindamycin. Seven isolates were resistant to gentamicin and harbored the aac(6 )-Ie-aph (2 )-Ia gene. Resistance to tetracycline was detected in 10 isolates harboring the tet(K) gene. The IEC genes were detected in three isolates. MRSA strains belonged to CC97, CC1, CC5, CC15 or CC22. The isolates were assigned to six different spa types. In this study we found a moderate prevalence of multidrug-resistant MRSA strains in food-producing rabbits. This may represent concern for food Foods 2020, 9, 439; doi:10.3390/foods9040439 www.mdpi.com/journal/foods Foods 2020, 9, 439 2 of 10 safety and public health, since cross-contamination may occur, leading to the spread of MRSA and, eventually, the possibility of ingestion of contaminated meat.

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Section: Characterization Of Cc97 Mrsa Isolatessupporting

confidence: 66%

Livestock-Associated Methicillin-Resistant Staphylococcus aureus (MRSA) in Purulent Subcutaneous Lesions of Farm Rabbits

Silva

Sousa

Gómez

et al. 2020

Foods

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“…Erythromycin is known to act by inhibition of protein synthesis. Several authors have also reported similar findings about Staphylococcus aureus from clinical samples showing resistance of different degrees [35][36][37]. According to Adekanmbi, Falodun antimicrobial resistance may be plasmid-coded and this may encourage transfer of the resistance genes to once-susceptible microorganism.…”

Section: Discussionmentioning

confidence: 73%

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2024

JIDTM

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Abattoirs are springing up in several locations within Port Harcourt to ease access to meat products as well as reduce cost of purchase and transportation. However, the presence of several pathogenic and antibiotic resistant microorganisms in abattoirs as a result of wastes generated in the process of preparations, have of recent been a public health concern as little or no care is taken in ensuring safe and proper disposal of abattoir wastes as well as purity of the service water used in abattoirs. In this study, samples (blood, water, swab and faeces) from three abattoirs in Port Harcourt were obtained and the isolation of Methicillin resistant Staphylococcus aureus was carried out using appropriate microbiological techniques. Methicillin resistance was determined using Cefoxitin and Oxacillin only while sensitivity tests were performed for other S. aureus isolates to different antibiotics using Gentamicin, Erythromycin, Augmentin, Ofloxacin, Cefuroxime, Ceftriaxone, Cloxacillin and Ceftazidime. Results obtained showed that MRSA was susceptible to Oxacillin with 39.6% and Cefoxitin with 44.4% from samples collected from Tran Amadi abattoir but showed intermediate resistance to isolates from Rumuodumaya and Iwofe abattoirs. The percentage occurrence of S. aureus was highest among the faecal samples with 58.3% while blood samples which is supposed to be sterile, was observed from this study to have staphylococcal counts of 1.4%. The multiple antibiotic sensitivity tests showed that S. aureus isolates were susceptible to Ofloxacin with 28mm as zones of inhibition; Gentamicin had 20mm and Ceftriaxone had 16mm while they were 100% resistant to Augmentin, Cefuroxime and Ceftazidime while erythromycin showed intermediate sensitivity. However, it was observed that out of the 144 Staphylococcus aureus isolates from the 3 sampling points, 22.2% were resistant to Cefoxitin while 26.4% were resistant to Oxacillin. This study suggests that different resistance genes may accumulate on a single mobile element, presenting a situation in which multiple antibiotic resistance can be acquired via a single genetic event. The versatility of bacterial populations in adapting to toxic environments, along with their facility in exchanging DNA, signifies that antibiotic resistance is an inevitable biological phenomenon that will likely continue to be a chronic medical problem.

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“…In this study, we investigated the prevalence of S. aureus in different food producing animals, including pigs, cattle, goats, rabbits and chicken, in Chongqing, China. To our understanding, this is the first comprehensive survey on livestock-associated S. aureus in Chongqing, although numerous epidemiological studies of this organism have been carried out in other regions of China [1,29,30], and a report about S. aureus isolated from goats in Chongqing [31] has been published. The average prevalence of S. aureus in our study was similar with those obtained from Jiangmen, China [30], but differed from other areas.…”

Section: Discussionmentioning

confidence: 99%

“…To our understanding, this is the first comprehensive survey on livestock-associated S. aureus in Chongqing, although numerous epidemiological studies of this organism have been carried out in other regions of China [1,29,30], and a report about S. aureus isolated from goats in Chongqing [31] has been published. The average prevalence of S. aureus in our study was similar with those obtained from Jiangmen, China [30], but differed from other areas. For instance, a study carried out in Henan showed that 23.7% of animal samples collected from cows, swine, chicken and ducks, were positive for S. aureu [1].…”

Section: Discussionmentioning

confidence: 99%

“…Although there have been a great deal of reports on the epidemiological studies of S. aureus originated from food producing animals in China in recent years [1,29,30], little is known about the prevalence and occurrence of this organism in Chongqing, China. In this study, we investigated the prevalence of S. aureus isolated from food animals in Chongqing.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence, antimicrobial resistance and staphylococcal toxin gene of blaTEM-1a-producing Staphylococcus aureus isolated from animals in Chongqing, China

Wang

Yao

Jia

et al. 2019

Preprint

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Background Livestock-associated Staphylococcus aureus is one of the most important etiological agents in both human and animals. It has been reported with high antimicrobial resistance and multiple staphylococcal superantigen genes in many countries and several provinces of China. However, large-scale investigation of this organism has not been documented in Chongqing, China. The aim of this study is to demonstrate the prevalence, antimicrobial susceptibility and some molecular characteristics of S. aureus acquired from animals in Chongqing. Results A total of 89 S. aureus isolates were cultured from 1371 samples picked up from March 2014 to December 2017. The isolates were originated from pigs (25), cattle (6), goats (10), rabbits (16) and chicken (32). Four MRSA strains were identified from 3 pig samples and 1 chicken sample. The isolates showed high resistance to penicillin (93.3%) and ampicillin (92.1%), but were more susceptible to amikacin and ofloxacin, since the resistance rates of these two drugs were less than 10%. Meanwhile, 74.2% isolates exhibited varying degree of MDR. Almost all strains, except for 3 chicken-originated isolates, were positive for blaTEM-1a, but did not harbor other ESBL genes. Nineteen staphylococcal SE/SEl/TSST-1 genes, except seq, were detected in isolates. The predominant genes were sei (58.4%), tst-1 (56.2%) and seg (51.7%). Conclusions The high antimicrobial resistance and prevalence of blaTEM-1a seriously reminded that it was urgent to standardize and cut down the usage of antimicrobials. The universal existence of staphylococcal toxin genes in isolated strains implied a potential threat of public health from animals to human through the food chain.

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Phenotypic and molecular characteristics of methicillin-resistant and methicillin-susceptible <em>Staphylococcus aureus</em> isolated from pigs: implication for livestock-association markers and vaccine strategies

Cited by 32 publications

References 57 publications

Livestock-Associated Methicillin-Resistant Staphylococcus aureus (MRSA) in Purulent Subcutaneous Lesions of Farm Rabbits

Livestock-Associated Methicillin-Resistant Staphylococcus aureus (MRSA) in Purulent Subcutaneous Lesions of Farm Rabbits

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Prevalence, antimicrobial resistance and staphylococcal toxin gene of blaTEM-1a-producing Staphylococcus aureus isolated from animals in Chongqing, China

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