Phenotypic and molecular characterisation of fish-borne Flavobacterium johnsoniae-like isolates from aquaculture systems in South Africa

Flemming, Leonard; Rawlings, Douglas E.; Chenia, Hafizah Y.

doi:10.1016/j.resmic.2006.09.001

Cited by 44 publications

(48 citation statements)

References 38 publications

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“…However, reports of 'less well-known' and novel Flavobacterium spp. being recovered from the internal organs of fish in Africa (Flemming et al 2007), Asia (Karatas et al 2010, Suebsing & Kim 2012, Europe (Zamora et al 2012(Zamora et al , 2013(Zamora et al , 2014, and South America (Kämpfer et al 2012) are beginning to surface. Thus, the findings of this study, in conjunction with the findings of the aforementioned studies, illustrate that a much more diverse assemblage of flavobacteria are capable of systemically infecting fish.…”

Section: Discussionmentioning

confidence: 99%

Deciphering the biodiversity of fish-pathogenic Flavobacterium spp. recovered from the Great Lakes basin

Loch¹,

Faisal²

2014

Dis. Aquat. Org.

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Flavobacterial diseases negatively impact wild and cultured fishes worldwide. We recently reported on the presence of a large and diverse group of flavobacteria, many of which were associated with lesions in a number of Great Lakes fish species. Herein, we report on the characterization of 65 fish-associated Flavobacterium spp. isolates using 16S rRNA gene sequence analysis and phylogenetic analyses based upon neighbor-joining and Bayesian methodologies. Thirteen isolates were identified as the newly described fish-associated F. plurextorum, F. spartansii, and F. tructae, while 3 isolates were similar to F. frigidimaris; however, the remaining Flavobacterium spp. isolates did not conclusively match any described Flavobacterium spp. and thus were suspected as comprising novel flavobacterial species. A more comprehensive polyphasic characterization was undertaken on 6 isolates, representing a range of association with disease signs in hatchery-raised or free-ranging fish and genetic distinctness. Polyphasic characterization included physiological, morphological, and biochemical analyses, as well as additional phylogenetic analyses based upon near-complete sequencing of the 16S rRNA gene. Our findings demonstrated that that at least 5 of the 6 isolates are most likely novel species within the genus Flavobacterium that have never before been reported from fish. Pilot experimental challenge studies suggested that some of these Flavobacterium spp. can cause pathological lesions in fish and were re-isolated from the brains, spleens, livers, and kidneys of experimentally infected fish. The findings underscore the growing number and heterogeneity of flavobacteria now known to be capable of infecting fish.

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Section: Discussionmentioning

confidence: 99%

Deciphering the biodiversity of fish-pathogenic Flavobacterium spp. recovered from the Great Lakes basin

Loch¹,

Faisal²

2014

Dis. Aquat. Org.

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“…Twelve (12) previously characterized F. johnsoniae-like isolates (YO12, YO15, YO19, YO34, YO45, YO51, YO53, YO59, YO60, YO63, YO64, and YO67) isolated from eel, koi-carp and trout were selected for study based on their biofilm-forming abilities (Flemming et al, 2007;Basson et al, 2008). Isolates were divided into two groups (Table 1) based on their colony morphotypes (Flemming et al, 2007), that is, smooth (YO19, YO34, YO51, YO59, YO63, YO64 and YO67) and hazy (YO12, YO15, YO45, YO53 and YO60).…”

Section: Bacterial Isolatesmentioning

confidence: 99%

“…Isolates were divided into two groups (Table 1) based on their colony morphotypes (Flemming et al, 2007), that is, smooth (YO19, YO34, YO51, YO59, YO63, YO64 and YO67) and hazy (YO12, YO15, YO45, YO53 and YO60). The associated motility characteristics, biofilm phenotypes and hydrophobicity of isolates are indicated in Table 1 (Flemming et al, 2007;Basson et al, 2008 …”

Section: Bacterial Isolatesmentioning

confidence: 99%

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Effect of physiochemical factors on autoaggregation and adhesion of Flavobacterium johnsoniae-like isolates

Chenia¹,

Chadwick²

2013

Afr. J. Microbiol. Res.

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Flavobacterium johnsoniae-like isolates are able to form biofilms and have been associated with disease outbreaks in fish. Surface colonization requires microbial adhesion strategies that are mediated by specific surface-associated molecules. Blocking or inhibiting these molecules would facilitate the identification of adhesins involved in surface adherence and development of appropriate anti-adhesion strategies for F. johnsoniae-like isolates. Autoaggregation and adhesion assays were performed using F. johnsoniae-like isolates and Flavobacterium spp. type strains to determine the anti-adhesion effect of heat, proteinase K, glucose, galactose, mannose, and sodium metaperiodate. Majority of the isolates (~85%) displayed decreased autoaggregation following heat treatment, while ~80% and 100% of isolates demonstrated increased autoaggegation following proteinase K and sodium metaperiodate treatments. Although carbohydrate treatments increased autoaggregation indices, they were not significant. Heat, proteinase K, sodium metaperiodate (SMP), glucose and galactose treatments significantly decreased adhesion of flavobacterial isolates to microtitre plates under static conditions, but not under dynamic conditions. Autoaggregation and surface adhesion by F. johnsoniae-like isolates appears to be mediated by chemically diverse groups of adhesins and receptors, given the diverse responses observed following treatment of cells. Glycoprotein molecules form part of the complement involved in mediating successful attachment of Flavobacterium spp. isolates to surfaces. To prevent attachment and recurring infections by F. johnsoniae-like isolates, understanding the type of adhesin-receptor interactions in adhesion is critical. This will allow the development of strategies to disrupt initial attachment and prevent biofilm formation by Flavobacterium spp.

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“…In identifying fi sh diseases, molecular techniques are potentially more sensitive than conventional methods such as culture, serology and histology [7]. Randomly amplifi ed polymorphic DNA (RAPD) fi ngerprinting blended with PCR is an important molecular diagnostic tool that can be adopted for quick and precise identifi cation of F. columnare and to compare strains of numerous bacterial species because of the generic capabilities of PCR system [2,8,9]. This method is quite simple, relatively economical, reliable and is particularly suitable when the quantity of the genomic DNA is limited and where prior information related to structure of the genomic DNA is lacking.…”

Section: Introductionmentioning

confidence: 99%

DNA fingerprinting of Flavobacterium columnare using RAPD-PCR

et al. 2010

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In the present study, DNA fi ngerprinting of eight strains of Flavobacterium columnare was done by random amplifi cation of polymorphic DNA (RAPD) fi ngerprinting method. The strains were collected from Fish Health Management Division, Central Institute of Freshwater Aquaculture, Bhubaneswar, India. A total number of 160 primers were screened for RAPD-PCR, of which 10 primers yielded amplifi cation with all the strains. The molecular weight of amplifi ed bands varied from 0.29-2.63 Kb. The number of bands varied from 1 to 8. Unique band was seen with primer OPY-15 with molecular weight 0.75 Kb that can be used for epidemiological study. Genetic variability was investigated using NTSYS software. Highest genetic similarity was found between MS1 and MS3 followed by MS5 and MS7. Minimum genetic similarity was found between MS2 and MS8. Phylogenetic tree was constructed using UPGMA and neighbor joining methods.

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Phenotypic and molecular characterisation of fish-borne Flavobacterium johnsoniae-like isolates from aquaculture systems in South Africa

Cited by 44 publications

References 38 publications

Deciphering the biodiversity of fish-pathogenic Flavobacterium spp. recovered from the Great Lakes basin

Deciphering the biodiversity of fish-pathogenic Flavobacterium spp. recovered from the Great Lakes basin

Effect of physiochemical factors on autoaggregation and adhesion of Flavobacterium johnsoniae-like isolates

DNA fingerprinting of Flavobacterium columnare using RAPD-PCR

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