Phenotypic and genotypic typing ofSalmonella enterica serovar Enteritidis isolates from poultry farms environments in Tunisia

Abstract

-Salmonella enterica serovar Enteritidis is one of the major Salmonella serovars which may cause animal infections and human salmonellosis. In this study, two hundred forty five samples (faeces, water and environmental swabs) were taken from eight poultry farms localized in different geographical areas of Tunisia. We found Salmonella serovar Enteritidis (16 strains), Salmonella typhimirium (2), Salmonella scharzengrund (2), and Salmonella braenderup (1). Salmonella Enteritidis strains were characterized by pulsed field gel electrophoresis (PFGE) analysis, plasmid analysis and antibiotic resistance profiles. XbaI PFGE analysis revealed two PFGE types and plasmid profiling identified four plasmid types. The majority of isolates were susceptible to all antibiotic tested. The combined use of phenotypic and genotypic methods indicates the spread of a particular Salmonella Enteritidis clone. This clone is highly related to a major world-wide clone identified in many other countries.

Quinolone resistance amongSalmonellaKentucky and Typhimurium isolates in Tunisia: first report ofSalmonellaTyphimurium ST34 in Africa andqnrB19in Tunisia

“…This is in contrast to various data from the Tunisian literature (Guedda et al . 2009; Turki et al . 2012; Al‐Gallas et al .…”

“…Limited information is available on the occurrence of the emerging Salmonella antibiotic resistance in Tunisia (Guedda et al . 2009; Abbassi‐Ghozzi et al . 2012; Turki et al .…”