Phenotypic and genotypic (pulsed-field gel electrophoresis) characteristics of enterotoxin-A-producing Staphylococcus aureus strains

Gouloumès, C.; Bes, M.; Renaud, François; Lina, Bruno; Reverdy, Marie-Élisabeth; Brun, Y.; Fleurette, J

doi:10.1016/0923-2508(96)81386-6

Cited by 7 publications

(3 citation statements)

References 28 publications

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“…To optimize the incubation time and the production yield of toxins, various factors, such as pH, osmotic pressure, and the use of substrates, are important ( 6 ). Many methods are based on the direct detection of enterotoxins in food, with the ability to detect enterotoxins at the nanogram scale in one gram or milliliter of food ( 7 , 8 ). Enterotoxins can be detected by enzyme-linked immunosorbent assays, chemiluminescence, or reversed passive latex agglutination tests.…”

Section: Introductionmentioning

confidence: 99%

Detection of Staphylococcus Enterotoxin B (SEB) Using an Immunochromatographic Test Strip

Gholamzad

Khatami

Ghassemi

et al. 2015

Jundishapur J Microbiol

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Background:Staphylococcus aureus is one of the most important microorganisms that causes various human diseases by secreting virulence factors known as staphylococcal super antigens (SAgs). Staphylococcal Enterotoxin B (SEB) is a bacterial antigen that is responsible for food poisoning in humans. Among SEB detection methods, a lateral flow device (LFD) is ideal for rapid immunochromatographic tests because it is easy to use, requires minimal time to produce results, and does not require personnel training.Objectives:In our laboratory, the production of an immunochromatographic test strip, for the detection of SEB using a sandwich assay and a competitive method, was described; the test can detect SEB with high sensitivity.Materials and Methods:The strip assays were compared with PCR, a valid method for detection. For PCR, a specific sequence for SEB production was detected using primers designed according to GenBank sequences.Results:In total, 80 food samples suspected of SEB contamination were assessed using the two methods. Fifty-four samples were contaminated based on the PCR technique and twenty-six of those were confirmed using the strip assay.Conclusions:The sensitivity of the sandwich method was approximately 10 ng/mL and that of the competitive method was approximately 250 ng/mL. In the LFD, a highly specific monoclonal antibody used for both the sandwich and competitive methods resulted in an increased sensitivity and accuracy for the detection of a minimal SEB concentration.

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Section: Introductionmentioning

confidence: 99%

Detection of Staphylococcus Enterotoxin B (SEB) Using an Immunochromatographic Test Strip

Gholamzad

Khatami

Ghassemi

et al. 2015

Jundishapur J Microbiol

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“…The enterotoxins pass from the digestive tract to the blood circulation and consecutively activate the nerve centre of the emetic reflex, causing nausea, vomiting, abdominal cramps and diarrhea, which can lead to dehydration and the collapse of the organism (Gouloumes et al 1996).…”

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confidence: 99%

“…Some factors, which affect the incubation period, are the pH, the water activity and the used substrates (Sharma et al 2000). Numerous methods are based on the evidence of the enterotoxins directly in the food (ELISA, reversal passive latex agglutination and others), with a possibility to detect nanogram amounts of enterotoxins in one gram or in one mili-liter of food (Burdová et al 1994;Gouloumes et al 1996;Strachan et al 1997). The advantage of these methods is that enterotoxins are detected even if the producer Staphylococcus aureus should not be identified by the classical bacteriological procedure, because it is usually devitalized by temperature.…”

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confidence: 99%

Detection of the Genes for Staphylococcus aureus Enterotoxins by PCR

Tkáčiková¹,

Tesfaye²,

Mikula³

2003

Acta Vet. Brno

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The aim of this study was to determine the presence of genes for staphylococcal enterotoxins in group of 32 isolates of Staphylococcus aureus from 75 samples (sheep, cow and goat raw milk) collected from sub-clinically and clinically sick sheep, goats and cows raised in different agricultural farms in the Eastern region of Slovakia and also from 12 samples of sheep cheeses. Fifteen out of 32 isolates of S. aureus carried gene for enterotoxin C (SEC): 12 from sheep raw milk and one from each of goat and cow raw milk and sheep cheese, too. Fifteen out of 32 isolates carried gene for enterotoxin D (SED): 12 from sheep raw milk, two from cow raw milk and one from sheep cheese. Two out of 32 isolates carried genes for enterotoxin A (SEA) and B (SEB): one positive isolate for SEA from sheep raw milk and the other SEB from sheep cheese.

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Computerized antibiogram for methicillin-resistantStaphylococcus aureus in chest surgery

Yoshida

Kondo

Akao

1999

Jpn J Thorac Caridovasc Surg

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Phenotypic and genotypic (pulsed-field gel electrophoresis) characteristics of enterotoxin-A-producing Staphylococcus aureus strains

Cited by 7 publications

References 28 publications

Detection of Staphylococcus Enterotoxin B (SEB) Using an Immunochromatographic Test Strip

Detection of Staphylococcus Enterotoxin B (SEB) Using an Immunochromatographic Test Strip

Detection of the Genes for Staphylococcus aureus Enterotoxins by PCR

Computerized antibiogram for methicillin-resistantStaphylococcus aureus in chest surgery

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