Phenotypic and genotypic differentiation in cage populations of Drosophila melanogaster. I. Duration of development, thorax size and weight

Lints, F.A.; Bourgois, M.

doi:10.1186/1297-9686-19-2-155

Cited by 11 publications

(3 citation statements)

References 27 publications

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“…2012 ; Salminen et al . 2019 ), we would recommend that attention should also be paid to mitochondrial genotype, in such back-crossing schemes, regardless of whether the expected phenotype is obviously “mitochondrial.” Note that the phenotypic differences observed in our back-crossed tko 25t lines are not the result of genetic heterogeneity within the wild-type lines, even though this may be considerable, as inferred previously for Oregon R ( Lints and Bourgois 1987 ). Back-crossed tko 25t lines within each background had relatively uniform phenotypes.…”

Section: Resultssupporting

confidence: 70%

Nuclear genetic background influences the phenotype of the Drosophila tko25t mitochondrial protein-synthesis mutant

Jacobs

Tuomela

Lillsunde

2023

G3: Genes, Genomes, Genetics

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The Drosophila tko25t point mutation in the gene encoding mitoribosomal protein S12 produces a complex phenotype of multiple respiratory chain deficiency, developmental delay, bang-sensitivity, impaired hearing, sugar and antibiotic sensitivity and impaired male courtship. Its phenotypic severity was previously shown to be alleviated by inbreeding, and to vary with mitochondrial genetic background. Here we show similarly profound effects conferred by nuclear genetic background. We backcrossed tko25t into each of two standard nuclear backgrounds, Oregon R and w1118, the latter used as recipient line in many transgenic applications requiring selection for the white minigene marker. In the w1118 background, tko25t flies showed a moderate developmental delay and modest bang-sensitivity. In the Oregon R background, males showed longer developmental delay and more severe bang-sensitivity, and we were initially unable to produce homozygous tko25t females in sufficient numbers to conduct a meaningful analysis. When maintained as a balanced stock over 2 years, tko25t flies in the Oregon R background showed clear phenotypic improvement though were still more severely affected than in the w1118 background. Phenotypic severity did not correlate with the expression level of the tko gene. Analysis of tko25t hybrids between the two backgrounds indicated that phenotypic severity was conferred by autosomal, X-chromosomal and parent-of-origin dependent determinants. Although some of these effects may be tko25t-specific, we recommend that, in order to minimize genetic drift and confounding background effects, the genetic background of non-lethal mutants should be controlled by regular backcrossing, even if stocks are usually maintained over a balancer chromosome.

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Section: Resultssupporting

confidence: 70%

Nuclear genetic background influences the phenotype of the Drosophila tko25t mitochondrial protein-synthesis mutant

Jacobs

Tuomela

Lillsunde

2023

G3: Genes, Genomes, Genetics

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show abstract

“…Given our previous observations of profound effects of different mtDNA backgrounds on tko 25t (Chen et al 2012;Salminen et al 2019), we would recommend that attention should also be paid to mitochondrial genotype, in such back-crossing schemes, regardless of whether the expected phenotype is obviously 'mitochondrial'. Note that the phenotypic differences observed in our back-crossed tko 25t lines is not the result of genetic heterogeneity within the wildtype lines, even though this may be considerable, as inferred previously for Oregon R (Lints and Bourgois 1987). Back-crossed tko 25t lines within each background had relatively uniform phenotypes.…”

Section: Concluding Commentssupporting

confidence: 71%

Nuclear genetic background influences the phenotype of theDrosophila tko^25tmitochondrial protein-synthesis mutant

Jacobs

Tuomela

Lillsunde

2023

Preprint

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TheDrosophila tko25tpoint mutation in the gene encoding mitoribosomal protein S12 produces a complex phenotype of multiple respiratory chain deficiency, developmental delay, bang-sensitivity, impaired hearing, sugar and antibiotic sensitivity and impaired male courtship. Its phenotypic severity was previously shown to be alleviated by inbreeding, and to vary with mitochondrial genetic background. Here we show similarly profound effects conferred by nuclear genetic background. We backcrossedtko25tinto each of two standard nuclear backgrounds, Oregon R andw1118, the latter used as recipient line in many transgenic applications requiring selection for thewhiteminigene marker. In thew1118background,tko25tflies showed a moderate developmental delay and modest bang-sensitivity. In the Oregon R background, males showed longer developmental delay and more severe bang-sensitivity, and we were initially unable to produce homozygoustko25tfemales in sufficient numbers to conduct a meaningful analysis. When maintained as a balanced stock over 2 years,tko25tflies in the Oregon R background showed clear phenotypic improvement though were still more severely affected than in thew1118background. Phenotypic severity did not correlate with the expression level of thetkogene. Analysis of tko25t hybrids between the two backgrounds indicated that phenotypic severity was conferred by autosomal, X-chromosomal and parent-of-origin dependent determinants. Although some of these effects may betko25t-specific, we recommend that, in order to minimize genetic drift and confounding background effects, the genetic background of non-lethal mutants should be controlled by regular backcrossing, even if stocks are usually maintained over a balancer chromosome.

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“…For instance, experiments on flies evolving in a constant thermal environment indicate that lines perform better for many traits at their own rearing temperature than do lines reared at cooler or warmer temperatures (Mourad, 1965;Cavicchi et ai., 1989;Huey et ai., 1991). The higher temperature lines also tolerate high-stress temperatures better than those reared at lower temperatures (Stephanou et ai., 1983;Kilias and Alahiotis, 1985;Lints and Bourgois, 1987;Cavicchi et ai., 1995). However, it is not known whether differences result from reduced tolerance to heat in lines evolving in a cold environment or to increased tolerance of those reared under warmer regimes.…”

Section: Acclimation To High Temperaturesmentioning

confidence: 98%

Environmental Stress, Adaptation and Evolution

1997

Experientia Supplementum

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Phenotypic and genotypic differentiation in cage populations of Drosophila melanogaster. I. Duration of development, thorax size and weight

Cited by 11 publications

References 27 publications

Nuclear genetic background influences the phenotype of the Drosophila tko25t mitochondrial protein-synthesis mutant

Nuclear genetic background influences the phenotype of the Drosophila tko25t mitochondrial protein-synthesis mutant

Nuclear genetic background influences the phenotype of theDrosophila tko^25tmitochondrial protein-synthesis mutant

Environmental Stress, Adaptation and Evolution

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Phenotypic and genotypic differentiation in cage populations of Drosophila melanogaster. I. Duration of development, thorax size and weight

Cited by 11 publications

References 27 publications

Nuclear genetic background influences the phenotype of the Drosophila tko25t mitochondrial protein-synthesis mutant

Nuclear genetic background influences the phenotype of the Drosophila tko25t mitochondrial protein-synthesis mutant

Nuclear genetic background influences the phenotype of theDrosophila tko25tmitochondrial protein-synthesis mutant

Environmental Stress, Adaptation and Evolution

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Product

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Nuclear genetic background influences the phenotype of theDrosophila tko^25tmitochondrial protein-synthesis mutant