Phenotypic and Genotypic Characterizations of Campylobacter jejuni Isolated from the Broiler Meat Production Process

Kudirkienė, Eglė; Cohn, Marianne Thorup; Stabler, Richard A.; Strong, Philippa C. R.; Šernienė, Loreta; Wren, Brendan W.; Nielsen, Eva Møller; Malakauskas, Mindaugas; Brøndsted, Lone

doi:10.1007/s00284-012-0170-z

Cited by 25 publications

(14 citation statements)

References 53 publications

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“…In addition, it has been suggested that the ability to adapt to environmental stress may be strain or genotype dependent and this may be genetically determined (Kudirkiene et al . ).…”

Section: Resultsmentioning

confidence: 97%

“…Second, the methods do not perform equally well with all sample types, so that using the optimal method per type introduces methodological variation that can introduce a bias in the genotypes that are detected. In addition, it has been suggested that the ability to adapt to environmental stress may be strain or genotype dependent and this may be genetically determined (Kudirkiene et al 2012). Table 3 summarizes the findings when two methods were compared for single samples, whereby the selective agar or the enrichment broth varied.…”

Section: Resultsmentioning

confidence: 99%

“…; Kudirkiene et al . ). The other approach is to follow multiple flocks, preferably from different farms so that they are colonized by different populations, at the cost of fewer samples being taken per production batch and per sampling point.…”

Section: Introductionmentioning

confidence: 97%

“…In order to investigate possible fluctuations in the bacterial populations during processing, large numbers of samples per sampling point need to be investigated, both qualitatively and quantitatively. As a consequence, only few such investigations have been performed, and it is difficult to judge how representative these studies are for other flocks or other slaughterhouses (Hanning et al 2010;Kudirkiene et al 2012). The other approach is to follow multiple flocks, preferably from different farms so that they are colonized by different populations, at the cost of fewer samples being taken per production batch and per sampling point.…”

Section: Introductionmentioning

confidence: 99%

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The effect of different isolation protocols on detection and molecular characterization of Campylobacter from poultry

Ugarte‐Ruiz

Wassenaar

Gómez-Barrero

et al. 2013

Lett Appl Microbiol

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Significance and Impact of Study: The tracing of Campylobacter through the food chain remains important to control campylobacteriosis in humans. Our study points out that the isolation method used affects the genotypes obtained, and this should be considered as a variant when comparing the results of surveillance studies. AbstractWe determined whether different methods to isolate Campylobacter (including the ISO standard 10272:2006-1) affected the genotypes detectable from poultry, at three points during slaughter: caecal content, neck skin and meat. Carcasses from 28 independent flocks were thus sampled (subset A). In addition, ten neck skin samples from four flocks, ten caecal samples from ten different flocks and ten unrelated meat samples obtained from local supermarkets were collected (subset B). Campylobacter was isolated using eight different protocols: with and without enrichment using Bolton broth, Preston broth or Campyfood broth (CFB), followed by culture on either modified Charcoal Cefoperazone Deoxycholate Agar (mCCDA) or Campyfood agar (CFA). All obtained isolates were genotyped for flaA-SVR, and over half of the isolates were also typed by MLST. The strain richness, as a measure of number of detected fla-genotypes, obtained from subset A neck skin and caecal samples was higher than that of meat samples. In half of the cases, within a flock, at least one identical fla-genotype was obtained at all three slaughter stages, suggestive of autologous contamination of carcasses. Enrichment reduced the observed richness of isolates, while CFA plates increased richness compared to mCCDA plates, irrespective of inclusion of an enrichment step. Because the isolation protocol used influences both the yield and the fla-genotype richness obtained from poultry, this variable should be taken into account when different studies are being compared.

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“…In addition, it has been suggested that the ability to adapt to environmental stress may be strain or genotype dependent and this may be genetically determined (Kudirkiene et al . ).…”

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The effect of different isolation protocols on detection and molecular characterization of Campylobacter from poultry

Ugarte‐Ruiz

Wassenaar

Gómez-Barrero

et al. 2013

Lett Appl Microbiol

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“…Biofilms were formed as described by Kudirkiene et al (2012), with modifications. Briefly, 200 μl of the bacterial suspension containing 10 4 cells prepared in MH broth and MH with 5% of chicken juice was added in 96-well plates.…”

Section: Methodsmentioning

confidence: 99%

Intrinsic and Extrinsic Aspects on Campylobacter jejuni Biofilms

et al. 2017

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Biofilm represents a way of life that allows greater survival of microorganisms in hostile habitats. Campylobacter jejuni is able to form biofilms in vitro and on surfaces at several points in the poultry production chain. Genetic determinants related to their formation are expressed differently between strains and external conditions are decisive in this respect. Our approach combines phylogenetic analysis and the presence of seven specific genes linked to biofilm formation in association with traditional microbiology techniques, using Mueller Hinton and chicken juice as substrates in order to quantify, classify, determine the composition and morphology of the biomass of simple and mixed biofilms of 30 C. jejuni strains. It also evaluates the inhibition of its formation by biocides commonly used in industry and also by zinc oxide nanoparticles. Genetic analysis showed high heterogeneity with the identification of 23 pulsotypes. Despite the diversity, the presence of flaA, cadF, luxS, dnaJ, htrA, cbrA, and sodB genes in all strains shows the high potential for biofilm formation. This ability was only expressed in chicken juice, where they presented phenotype of a strong biofilm producer, with a mean count of 7.37 log CFU/mL and an ultrastructure characteristic of mature biofilm. The composition of simple and mixed biofilms was predominantly composed by proteins. The exceptions were found in mixed biofilms with Pseudomonas aeruginosa, which includes a carbohydrate-rich matrix, lower ability to sessile form in chicken juice and compact architecture of the biofilm, this aspects are intrinsic to this species. Hypochlorite, chlorhexidine, and peracetic acid were more effective in controlling viable cells of C. jejuni in biofilm, but the existence of tolerant strains indicates exposure to sublethal concentrations and development of adaptation mechanisms. This study shows that in chicken juice C. jejuni presents greater potential in producing mature biofilms.

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Biofilm Development by Campylobacter Jejuni

Sung

Khan

2015

Biofilms in the Food Environment

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Phenotypic and Genotypic Characterizations of Campylobacter jejuni Isolated from the Broiler Meat Production Process

Cited by 25 publications

References 53 publications

The effect of different isolation protocols on detection and molecular characterization of Campylobacter from poultry

The effect of different isolation protocols on detection and molecular characterization of Campylobacter from poultry

Intrinsic and Extrinsic Aspects on Campylobacter jejuni Biofilms

Biofilm Development by Campylobacter Jejuni

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