Phenotypic and genotypic characterization of Cryptosporidium species and isolates

Carreno, Ramon A.; Pokorny, Nicholas J.; Lee, H; Trevors, J. T.; Grandis, Stephanie A. De

doi:10.1038/sj.jim.7000088

Cited by 19 publications

(9 citation statements)

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“…Several review articles have concluded that the degree of sequence divergence is difficult to interpret in a taxonomic context (Carreno et al 2001;Kunz 2002). Nevertheless, SSU rRNA gene sequences have been widely used in searching for evidence of distinct species as well as inferring phylogenetic relationships within genera.…”

Section: Discussionmentioning

confidence: 99%

Redescription of Trypanosoma siniperca Chang 1964 from freshwater fish of China based on morphological and molecular data

Wang

et al. 2006

Parasitol Res

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During the parasite fauna investigation within 2005, the freshwater fish trypanosome Trypanosoma siniperca Chang 1964 was isolated from the blood of Mandarin carp (Siniperca chuatsi) from Niushan Lake, Hubei Province, central China. Blood trypomastigotes were observed only, and the density of infection was low. Light microscopy examinations of this material made it possible to study in detail the morphology of this parasite and redescribe it according to current standards. T. siniperca is characterized also on the molecular level using the sequences of SSU rRNA gene. Phylogenetic analyses based on these sequences allowed clearer phylogenetic relationships to be established with other fish trypanosomes sequenced to date.

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Section: Discussionmentioning

confidence: 99%

Redescription of Trypanosoma siniperca Chang 1964 from freshwater fish of China based on morphological and molecular data

Wang

et al. 2006

Parasitol Res

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“…Taxonomically, the 11 following species are known : C. parvum, C. wrairi, C. meleagridis, C. saurophilum, C. felis, C. canis, C. baileyi, C. muris, C. andersoni, C. serpentis, C. nasorum [3,7,8]. Cryptosporidium parvum has been considered to be the only species that infects immunocompetent humans [5,9].…”

Section: Introductionmentioning

confidence: 99%

Genotyping of Cryptosporidium spp. isolated from human stool samples in Switzerland

et al. 2003

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SUMMARYIn a study to estimate the frequency of Cryptosporidium infections in Switzerland, stool samples from patients found to be positive for Cryptosporidium spp. by modified Ziehl-Neelson staining and fluorescence microscopy were used for genotyping experiments. With 9 of 12 samples, DNA extraction and subsequent genotyping was successful. All Cryptosporidium-isolates belonged to the bovine genotype. In one stool sample, two strains of Cryptosporidium were demonstrated, suggesting a mixed infection. In comparison with reference strains from calves, one of the isolates showed a full sequence identity and the other a similarity of 97 . 5%. The fact that only bovine genotypes were detected suggests, that cryptosporidiosis must primarily be considered as a zoonotic disease in Switzerland. This is in contrast to other countries, where the human genotype of C. parvum was shown to dominate the epidemiological situation. The results of our study are supported by the previous finding, that two of the analysed strains originated from patients who used to consume raw milk or raw cream, a known risk factor for cryptosporidiosis.

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“…Several species are now recognized within the genus Cryptosporidium, a protozoon associated largely with enteric infections in all classes of vertebrates (5,7,13,14,25,26). Mammals are commonly infected by either Cryptosporidium parvum (intestinal) or Cryptosporidium muris (gastric).…”

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confidence: 99%

Characterization of Cryptosporidium meleagridis of Human Origin Passaged through Different Host Species

et al. 2003

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Cryptosporidium meleagridis, a protozoon first observed in turkeys, has been linked by several investigators to cryptosporidiosis in humans. C. meleagridis is the only known Cryptosporidium species that infects both avian and mammalian species. We describe the successful propagation of C. meleagridis (isolate TU1867), originally purified from a patient with diarrhea, in laboratory animals including chickens, mice, piglets, and calves. TU1867 was readily transmitted from one animal host to another, maintaining genetic homogeneity and stability. The rate of infectivity and virulence of TU1867 for the mammalian species were similar to those of Cryptosporidium parvum. Laboratory propagation of genetically and phenotypically stable and well-characterized reference isolates, representing various Cryptosporidium species, particularly those infectious to humans, will improve considerably the spectrum and quality of laboratory and field investigations on this medically important protozoa.

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Phenotypic and genotypic characterization of Cryptosporidium species and isolates

Cited by 19 publications

References 0 publications

Redescription of Trypanosoma siniperca Chang 1964 from freshwater fish of China based on morphological and molecular data

Redescription of Trypanosoma siniperca Chang 1964 from freshwater fish of China based on morphological and molecular data

Genotyping of Cryptosporidium spp. isolated from human stool samples in Switzerland

Characterization of Cryptosporidium meleagridis of Human Origin Passaged through Different Host Species

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