Phenotypic and genotypic characterization of temporally related nontyphoidal Salmonella strains isolated from humans and food animals in central Ethiopia

Eguale, Tadesse; Asrat, Daniel; Alemayehu, Haile; Nana, Ismael; Gebreyes, Wondwossen A.; Engidawork, Ephrem

doi:10.1111/zph.12490

Cited by 23 publications

(26 citation statements)

References 35 publications

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“…Obesity has traditionally been regarded as an excessive energy state associated with diet and lifestyle. However, recent investigations indicate that obesity is a metabolic syndrome of complicated pathogenesis associated with genetic3–5 and environmental5–7 factors. Recently, the roles of estrogen, estrogen receptors (ERs) and the G protein-coupled estrogen receptor (GPER, previously termed GPR30) have garnered increasing attention.…”

Section: Introductionmentioning

confidence: 99%

Risk analysis of systemic levels of estrogen and adipokines as well as estrogen receptors from PBMCs in childbearing and perimenopausal women with obesity

Huang

Chen

Gao

et al. 2019

DMSO

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Purpose We aimed to evaluate the clinical value of systemic levels of estrogen and adipokines as well as estrogen receptors from peripheral blood mononuclear cells (PBMCs) in childbearing and perimenopausal women with obesity. Subjects and methods We observed 292 women, including 160 perimenopausal women (80 with obesity and 80 without obesity) and 132 women of childbearing age (67 with obesity and 65 without obesity). Body parameters, such as body mass index and waist circumference, were measured. Fat distribution was evaluated using a computerized tomography scanner. The levels of serum estrogen, leptin, visfatin, and adiponectin were measured using an enzyme-linked immunosorbent assay. The expression of circulating ERs was evaluated by Western blot analysis. Results Perimenopausal women and childbearing women with obesity exhibited lower levels of estrogen and adiponectin, in addition to a distribution of visceral fat with higher levels of leptin and visfatin. These findings reflect the current data of menopausal women, which confirms the reliability of this experimental system. However, the expression of ERα in peripheral blood was significantly enhanced in women with obesity of both childbearing and perimenopausal age. This result is contrary to the common understanding of adipose tissue, namely that ERα is protective. The expression of ERβ in the women without obesity of both childbearing and perimenopausal age was higher than in women with obesity, which coincides with the results of a previous study on adipose tissue. Conclusion Our data fundamentally contradicts the utility of circulating ERα and ERα/ERβ evaluations in obesity studies. Because estrogen exerts pleiotropic effects on multiple tissues in the body through differential regulation of ERs, although the expression of ERβ coincides with the results of a previous study on adipose tissue, the expression levels of ERs in blood cannot be used as a diagnostic of informative tool for obesity in women.

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Section: Introductionmentioning

confidence: 99%

Risk analysis of systemic levels of estrogen and adipokines as well as estrogen receptors from PBMCs in childbearing and perimenopausal women with obesity

Huang

Chen

Gao

et al. 2019

DMSO

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“…of Gram-negative isolates No. of ESBL (%) Abayneh M et al, 2018 [ 32 ] March to June 2016 Jimma 342 urine 74 17 (23.0) Abera B et al, 2016 [ 33 ] Sept 2013 to March 2015 Bahir Dar 477 blood, urine, pus/swab and body fluids 210 120 (57.1) Beyene G et al, 2011 [ 21 ] Jan to Aug. 2006 Addis Ababa 1225 stool, blood 113 78 (69.0) Desta K et al, 2016 [ 22 ] 10–20 Dec 2012 Addis Ababa 267 stool 295 151 (51.2) Eugale T et al, 2018 [ 23 ] April 2013 to March 2014 Addis Ababa 68 Stool 68 12 (17.6) Gashaw M et al, 2018 [ 24 ] May to Sept. 2016 Jimma 197 urine, swab/pus, blood, and sputum 100 36 (36.0) Legese et al, 2017 [ 25 ] Jan. to March 2014 Addis Ababa 322 blood and urine 28 22 (78.6) Mulisa et al, 2015 [ 26 ] April to Aug. 2016 Adama 384 urine, stool, swabs, and body fluids 65 17 (26.0) Mulualem Y et al, 2012 [ 27 ] Feb. to March 2007 Jimma 359 urine, stool, swabs, and sputum 67 24 (35.8) Pritsch M et al, 2017 [ 28 ] Jan. 2014 to June 2015 Jimma 224 ...…”

Section: Resultsmentioning

confidence: 99%

“…All of those were positive for ESBL-encoding genes: 82.7% (67/81) carried CTX-M-1 group and 17.3% (14/81) TEM. All CTX-M-1 group ESBL genes reported from included studies were confirmed to be CTX-M-15 genes [ 8 , 23 ]. Additional genes detected were: one isolate carrying a bla OXA gene from Addis Ababa, two bla CTX of the CTX-M-9 group [ 23 ] and three bla SHV genes only expressed in Enterobacter cloacae isolated in Jimma [ 8 ].…”

Section: Resultsmentioning

confidence: 99%

“…Of these, 8 were excluded because ESBL phenotypes or genotypes were not clearly described and 2 publications were identified as systematic reviews. Finally, 14 articles fulfilled eligibility criteria and were subjected to meta-analysis [8,[21][22][23][24][25][26][27][28][29][30][31][32][33] (Fig. 1).…”

Section: Distribution Of Articles Describing Esbl In Ethiopiamentioning

confidence: 99%

“…Out of 14 reviewed articles, only three (21.4%) studies, one from Addis Ababa University Black Lion Hospital and two from Jimma University Specialized Hospital, reported on the underlying resistance genes for the detection of ESBL resistance [8,23,28]. A total of 81 isolates were molecular-biologically analyzed for genetic resistance mechanism.…”

Section: Molecular Epidemiology Of Esbl In Ethiopiamentioning

confidence: 99%

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High rate of extended-spectrum beta-lactamase-producing gram-negative infections and associated mortality in Ethiopia: a systematic review and meta-analysis

Tufa

Fuchs

Tufa

et al. 2020

Antimicrob Resist Infect Control

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Background: Extended-spectrum beta-lactamase (ESBL)-producing Gram-negative bacteria have become a serious threat to global health. Their rapid spread is associated with high mortality due to ineffective antibiotic treatment. To date a regular surveillance of multidrug-resistant (MDR) pathogens in Ethiopia is not established. For this report, published data regarding ESBL-producing bacteria in different health facilities of Ethiopia were reviewed. Methods: This study collates data from published information on the rates and clinical implications of infection with ESBL-producing Gram-negative bacteria in Ethiopia. A systematic literature search was conducted using PubMed, PubMed Central, Medline, Science Direct and Google scholar from October 2018 to March 2019. Eligible studies were identified by applying quality criteria. The pooled proportion of ESBL-producing Gram-negative bacteria was estimated based on a random effect model. The publication bias and the variation in proportion estimates attributed to heterogeneity were assessed. Results: Fourteen studies with relevant data were included in the review. In total, 1649 Gram-negative bacteria isolated from 5191 clinical samples were included. The pooled proportion estimate of ESBL-producing Gramnegative bacteria was 50% (95% CI: 47.7-52.5%. Data showed a high level of heterogeneity (I 2 = 95%, P < 0.01). ESBL rates varied by species; 65.7% (263/400) in Klebsiella spp., 48.4% (90/186) in Salmonella spp., and 47.0% (383/ 815) in E. coli. ESBL-encoding genes were reported in 81 isolates: 67 isolates harbored the CTX-M-1 group and 14 isolates TEM. The mortality associated with infections by bacteria resistant to third generation cephalosporins has rarely been investigated. However, two studies reported a mortality of 33.3% (1/3) and 100% (11/11).

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Prevalence and antimicrobial susceptibility of Salmonella in poultry farms and in‐contact humans in Adama and Modjo towns, Ethiopia

et al. 2020

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Consumption of contaminated poultry and poultry products represents a common source of nontyphoidal Salmonella infection. Little is known on the status of Salmonella and their antimicrobial susceptibility in poultry farms in Ethiopia. This study investigated the prevalence, serotype distribution, and antimicrobial susceptibility of nontyphoidal Salmonella among poultry farms in Adama and Modjo towns. Three hundred thirty‐four cloacal swabs, 384 fecal droppings of birds, 59 feed, 59 floor swabs, and 36 stools from in‐contact humans were collected and processed for Salmonella isolation. Isolates were tested for their susceptibility to 15 antimicrobials using Kirby–Bauer disk diffusion assay. Seventeen (28.8%) of the farms and 24 (2.9%) of the samples from poultry farms and 2.8% (1/36) of stool samples of humans in‐contact with poultry were positive for Salmonella . Most of the isolates ( n = 21) were recovered from fecal droppings of birds while the remaining isolates were recovered from floor swab samples ( n = 2) and cloacal swab sample ( n = 1). Only three Salmonella serovars: S. Haifa ( n = 14, 56%), S. Anatum ( n = 7; 28%), and S. Give ( n = 4; 16%) were detected. Poultry farms in Adama town, large flock sized farms, and farms that used antimicrobials were significantly associated with the occurrence of Salmonella ( p < .05). Twenty (80%) and 19 (76%) of Salmonella isolates were resistant to streptomycin and tetracycline, respectively. Nineteen (76%) of the isolates were resistant to two or more antimicrobials. Detection of multidrug‐resistant strains of Salmonella in poultry farms suggests the need for detailed epidemiological and molecular studies to establish sources of acquisition of resistant Salmonella strains.

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Phenotypic and genotypic characterization of temporally related nontyphoidal Salmonella strains isolated from humans and food animals in central Ethiopia

Cited by 23 publications

References 35 publications

<p>Risk analysis of systemic levels of estrogen and adipokines as well as estrogen receptors from PBMCs in childbearing and perimenopausal women with obesity</p>

<p>Risk analysis of systemic levels of estrogen and adipokines as well as estrogen receptors from PBMCs in childbearing and perimenopausal women with obesity</p>

High rate of extended-spectrum beta-lactamase-producing gram-negative infections and associated mortality in Ethiopia: a systematic review and meta-analysis

Prevalence and antimicrobial susceptibility of Salmonella in poultry farms and in‐contact humans in Adama and Modjo towns, Ethiopia

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