Phenotypic and genotypic characterization of antimicrobial resistant Escherichia coli isolated from ready-to-eat food in Singapore using disk diffusion, broth microdilution and whole genome sequencing methods

Sufang, Guo; Tay, Moon Y. F.; Aung, Kyaw Thu; Seow, Kelyn Lee Ghee; Ng, Lee Ching; Purbojati, Rikky W.; Drautz–Moses, Daniela I.; Schuster, Stephan C.; Schlundt, Jørgen

doi:10.1016/j.foodcont.2018.12.043

Cited by 39 publications

(33 citation statements)

References 65 publications

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“…Our observation of tet(A) being the most common tetracycline resistance gene in all MDR isolates was in agreement with other studies in birds and rodents [ 26 , 39 , 40 ]. Isolate C1797 from wild bird was detected with the tet(B) gene—another gene which codes for efflux pump to transport Tetracycline out of bacterial cell [ 7 ]. Isolate C1722 from wild bird harboured tet(X) , which is the first described tetracycline resistance gene that encodes for an enzyme which inactivates Tetracycline.…”

Section: Discussionmentioning

confidence: 99%

“…Resistance to Ceftriaxone by disk diffusion was confirmed for ESBL production, as previously described [ 7 ].…”

Section: Methodsmentioning

confidence: 99%

“…Based on phenotypic resistance results, eight MDR E. coli isolates (indicated in Table 1 ) were selected and subjected to genotypic analysis by whole genome sequencing. DNA extraction, library preparation and sequencing were performed as previously described [ 7 ]. Raw sequence data was deposited into Genbank under Bioproject accession number PRJNA625931.…”

Section: Methodsmentioning

confidence: 99%

“…The World Health Organization (WHO) has categorized Enterobacteriaceae (including E. coli ) as pathogens of critical priority for antimicrobial resistance (AMR) investigation [ 6 ]. AMR in E. coli has been investigated in clinical settings and retail cooked food in Singapore [ 7 , 8 ]. However, to our knowledge, there is limited information on AMR in the environment such as in wild birds and rodents in Singapore.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence and Antimicrobial Resistance Traits of Escherichia coli from Wild Birds and Rodents in Singapore

Ong

Khor²,

Quek

et al. 2020

IJERPH

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Antimicrobial resistance (AMR) in Escherichia coli (E. coli) poses a public health concern worldwide. Wild birds and rodents, due to their mobility, are potential vehicles for transmission of AMR bacteria to humans. Ninety-six wild birds’ faecal samples and 135 rodents’ droppings samples were collected and analysed in 2017. Forty-six E. coli isolates from wild birds and rodents were subjected to AMR phenotypic and genotypic characterisation. The proportion of E. coli isolates resistant to at least one of the antimicrobials tested from wild birds (80.8%) was significantly higher than that of isolates from rodents (40.0%). The proportion of E. coli isolates resistant to each antimicrobial class for wild birds was 3.8% to 73.1% and that for rodents was 5.0% to 35.0%. Six out of 26 E. coli isolates from wild birds (23.1%) and two out of 20 (10.0%) isolates from rodents were multi-drug resistant (MDR) strains. These MDR E. coli isolates were detected with various antimicrobial resistance genes such as blaTEM-1B and qnrS1 and could be considered as part of the environmental resistome. Findings in this study suggested that wild birds and rodents could play a role in disseminating antimicrobial resistant E. coli, and this underscores the necessity of environment management and close monitoring on AMR bacteria in wild birds and rodents to prevent spreading of resistant organisms to other wildlife animals and humans.

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Section: Discussionmentioning

confidence: 99%

“…Resistance to Ceftriaxone by disk diffusion was confirmed for ESBL production, as previously described [ 7 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Occurrence and Antimicrobial Resistance Traits of Escherichia coli from Wild Birds and Rodents in Singapore

Ong

Khor²,

Quek

et al. 2020

IJERPH

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“…In another recent study, E. coli isolated from food-producing animals of two poultry farms in Brazil showed high resistance to tetracycline, nalidixic acid, ciprofloxacin, and levofloxacin (62). Moreover, E. coli isolated from chicken-based ready-to-eat foods demonstrated commonly resistance to tetracycline, ampicillin, and chloramphenicol in Singapore (61). Furthermore, E. coli isolates from local chicken meat products were considerably resistant to tetracycline, sulphonamide, ampicillin, and trimethoprim compared to imported chicken meat in Ghana (63), while in China E. coli isolated from chickens indicated resistance to oxytetracycline, amoxicillin, doxycycline, lomefloxacin, ceftriaxome, ofloxacin, enrofloxacin, and florfenicol, which is a distinct phenotypic antibiotic resistance profile in some degree compared to the former ones (64).…”

Section: Phenotypic and Genotypic Antimicrobial Resistance Analysis Omentioning

confidence: 97%

Isolation and molecular characterization of Salmonellaenterica and Escherichiacolifrom poultry samples

Cesur¹,

Ulutaş²,

Soyer³

2019

Turk J Vet Anim Sci

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Introduction Food might act as a crucial vehicle for transmission of illnesses from animals to humans. Foodborne zoonotic pathogens, mainly Campylobacter spp., Salmonella spp., and Shiga toxin producing E. coli, accommodate in intestinal tract of chicken, cattle, and swine and may induce foodborne diseases (1,2). Zoonotic bacteria present in poultry pose a major risk for both poultry industry and human health by increasing antibiotic resistance and contamination. To illustrate, more than 50 Salmonella infections in live poultry were observed, resulting in 2630 illnesses, 387 hospitalizations, and 5 deaths in the USA from 1999 to 2014 (3). Moreover, avian pathogenic E. coli might lead to serious flock mortality (4). In addition, E. coli outbreaks occurred in France and Germany in 2011 due to verocytotoxin producing E. coli. A total of 3126 cases and 17 deaths related to this bacterium were reported in Germany and the European Union (EU) (5). Salmonellosis, a nontyphoidal Salmonella infection, has been gradually increasing in Turkey and in other countries as a consequence of consuming poultry meat and its derivatives. Salmonellosis caused by Salmonella enterica subsp. enterica might lead to symptoms such as gastrointestinal infections and bloody diarrhea within 12 to 72 h (6). The most common Salmonella enterica serovars are Enteritidis, Typhimurium, and Infantis isolated from broilers, turkeys, pig meat, and human sources in Europe (7), however, in the USA, Kentucky, Enteritidis, Montevieo, Typhimurium and Infantis are frequently observed serotypes in animal products (8). According to the European Food Safety Authority and the European Centre for Disease Prevention and Control, among a total of 4786 foodborne outbreaks, Salmonella has been the most frequently detected foodborne pathogen, including Salmonella serovar Enteritidis and Infantis (9). Furthermore, the most significant increase in Salmonella infections was due to serotype Infantis in 2016 (10). Although Turkey occupies an important position in exporting chicken meat products, the data related to foodborne infections in Turkey is inadequate. Global food trading has expedited the emergence and spread of antibiotic resistant Salmonella. Hence, our study might provide useful information to trace the footprints of Salmonella outbreaks originating from the poultry products in Turkey.

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Antimicrobial susceptibility and genotypic characterization of Escherichia coli isolated from foods controlled by the National Food Safety Agency in Burkina Faso

Soubeiga,

Kpoda,

Sawadogo

et al. 2024

J Consum Prot Food Saf

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Phenotypic and genotypic characterization of antimicrobial resistant Escherichia coli isolated from ready-to-eat food in Singapore using disk diffusion, broth microdilution and whole genome sequencing methods

Cited by 39 publications

References 65 publications

Occurrence and Antimicrobial Resistance Traits of Escherichia coli from Wild Birds and Rodents in Singapore

Occurrence and Antimicrobial Resistance Traits of Escherichia coli from Wild Birds and Rodents in Singapore

Isolation and molecular characterization of Salmonellaenterica and Escherichiacolifrom poultry samples

Antimicrobial susceptibility and genotypic characterization of Escherichia coli isolated from foods controlled by the National Food Safety Agency in Burkina Faso

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