Phenotypic and genotypic characterisation of antimicrobial resistance in faecal bacteria from 30 Giant pandas

Zhang, Anyun; Wang, Hongning; Tian, Guo‐Bao; Zhang, Yi; Yang, Xin; Xia, Qingqing; Tang, Junni; Zou, Likou

doi:10.1016/j.ijantimicag.2008.10.030

Cited by 41 publications

(30 citation statements)

References 14 publications

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“…For antibiotic resistance of E. coli isolates from pandas, Zhang et al (31) reported that 32% (19/59) of the fecal bacteria from pandas were resistant to at least one antimicrobial and that 17% (10/59) were resistant to three or more antimicrobials, rates which were lower than the rates found in this study (76% and 36%, respectively). Compared to another report of antimicrobial resistances of 38 fecal E. coli strains isolated from pandas living in the same area in 2008 (32), the percent resistances to sulfamethoxazole-trimethoprim (from 0% to 48%) and gentamicin (from 3% to 13%) increased.…”

Section: Discussioncontrasting

confidence: 83%

Serotypes, Virulence Factors, and Antimicrobial Susceptibilities of Vaginal and Fecal Isolates of Escherichia coli from Giant Pandas

Wang

Yan

Xia

et al. 2013

Appl Environ Microbiol

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f Although Escherichia coli typically colonizes the intestinal tract and vagina of giant pandas, it has caused enteric and systemic disease in giant pandas and greatly impacts the health and survival of this endangered species. In order to understand the distribution and characteristics of E. coli from giant pandas, 67 fecal and 30 vaginal E. coli isolates from 21 giant pandas were characterized for O serogroups, phylogenetic groups, antimicrobial susceptibilities, and pulsed-field gel electrophoresis (PFGE) profiles. In addition, these isolates were tested for the presence of extraintestinal pathogenic E. coli (ExPEC) and diarrheagenic E. coli (DEC) by multiplex PCR detection of specific virulence genes. The most prevalent serogroups for all E. coli isolates were O88, O18, O167, O4, and O158. ExPEC isolates were detected mostly in vaginal samples, and DEC isolates were detected only in fecal samples. Phylogenetic group B1 predominated in fecal isolates, while groups B2 and D were frequently detected in vaginal isolates. Resistance to trimethoprim-sulfamethoxazole was most frequently observed, followed by resistance to nalidixic acid and tetracycline. All except five isolates were typeable by using XbaI and were categorized into 74 PFGE patterns. Our findings indicate that panda E. coli isolates exhibited antimicrobial resistance, and potentially pathogenic E. coli isolates were present in giant pandas. In addition, these E. coli isolates were genetically diverse. This study may provide helpful information for developing strategies in the future to control E. coli infections of giant pandas.

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Section: Discussioncontrasting

confidence: 83%

Serotypes, Virulence Factors, and Antimicrobial Susceptibilities of Vaginal and Fecal Isolates of Escherichia coli from Giant Pandas

Wang

Yan

Xia

et al. 2013

Appl Environ Microbiol

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“…Twenty-four pairs of specific primers were designed according to known sequences and references in GenBank [20][21][22][23][24][25][26][27][28]. The primers used for PCR and the respective amplification lengths are shown in Table 1.…”

Section: Detection Of the Class Integron Cassette And Resistancementioning

confidence: 99%

Molecular and Phenotypic Characteristics ofEscherichia coliIsolates from Farmed Minks in Zhucheng, China

Qiu

Jiang

Zhang

et al. 2019

BioMed Research International

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In this study, the prevalence, phenotypes, and clonal relationships of Escherichia coli (E. coli) strains isolated from minks were investigated. In July 2017, a total of 62 fresh faecal swab samples were randomly collected from one large-scale mink farm in Zhucheng, Shandong Province, China. In all the samples, 50 E. coli strains were isolated and then assigned to serotyping, antimicrobial susceptibility test, detection of antimicrobial resistance genes and the Class 1 integrons, and multilocus sequence typing (MLST). Four pathogenic serotypes were identified among all the isolates, while the most common serotype was enterohemorrhagic E. coli O104:H4 (6.0 %). Antimicrobial sensitivity testing revealed that most isolates were susceptible to cefoxitin (96.0 %) and amikacin (82.0 %), while most isolates were resistant to ampicillin (92.0 %) and tetracycline (90.0 %). An analysis of the nucleotide sequences revealed that 7 isolates (14.0%) carried 4 types of Class 1 integron cassette, including dfrA27+aadA2+qnrA (57.1%), dfrA17+aadA5 (14.3%), dfrA12+aadA2 (14.3%), and dfrA1+aadA1 (14.3%). PCR screening showed that 14 antibiotic resistance genes were presented in 50 isolates, while the most prevalent resistance gene was qnrS, which was detected in 60.0 % of isolates, followed by sul2 (40.0%) and oqxA (38.0%). MLST analysis showed that 32 sequence types (STs) were identified, while ST46 was the predominant genotype among all isolates. Clonal complex 3 (CC3) was dominant. Compared with 340 human E. coli STs reported in China, the ST10 clonal complex, known as the largest human clonal complex, was also found in the 50 mink E. coli isolates. Meanwhile, mink-derived strain ST206 formed a new clonal complex, CC206, which was different from human ST strains. Our results showed that farmed minks could be reservoirs of antimicrobial-resistant E. coli with Class 1 integron cassettes and resistance genes, which were likely to pose a threat to public health. Therefore, continuous inspections and monitoring of E. coli in minks are essential for detecting and controlling emerging E. coli with different serovars as well as antibiotic resistance.

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“…These animals may have close contact to human or farming areas and/or waste and become colonized with resistant strains (Literak et al, 2011; Nkogwe et al, 2011). Interestingly, animals in remote areas have been found to harbor-resistant bacteria (Zhang et al, 2009; Glad et al, 2010; Lang et al, 2010). …”

Section: The Veterinary Medicine and Agriculture Sectormentioning

confidence: 99%

A brief multi-disciplinary review on antimicrobial resistance in medicine and its linkage to the global environmental microbiota

Cantas¹,

Shah²,

Cavaco³

et al. 2013

Front. Microbiol.

298

211

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The discovery and introduction of antimicrobial agents to clinical medicine was one of the greatest medical triumphs of the 20th century that revolutionized the treatment of bacterial infections. However, the gradual emergence of populations of antimicrobial-resistant pathogenic bacteria resulting from use, misuse, and abuse of antimicrobials has today become a major global health concern. Antimicrobial resistance (AMR) genes have been suggested to originate from environmental bacteria, as clinically relevant resistance genes have been detected on the chromosome of environmental bacteria. As only a few new antimicrobials have been developed in the last decade, the further evolution of resistance poses a serious threat to public health. Urgent measures are required not only to minimize the use of antimicrobials for prophylactic and therapeutic purposes but also to look for alternative strategies for the control of bacterial infections. This review examines the global picture of antimicrobial resistance, factors that favor its spread, strategies, and limitations for its control and the need for continuous training of all stake-holders i.e., medical, veterinary, public health, and other relevant professionals as well as human consumers, in the appropriate use of antimicrobial drugs.

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Phenotypic and genotypic characterisation of antimicrobial resistance in faecal bacteria from 30 Giant pandas

Cited by 41 publications

References 14 publications

Serotypes, Virulence Factors, and Antimicrobial Susceptibilities of Vaginal and Fecal Isolates of Escherichia coli from Giant Pandas

Serotypes, Virulence Factors, and Antimicrobial Susceptibilities of Vaginal and Fecal Isolates of Escherichia coli from Giant Pandas

Molecular and Phenotypic Characteristics ofEscherichia coliIsolates from Farmed Minks in Zhucheng, China

A brief multi-disciplinary review on antimicrobial resistance in medicine and its linkage to the global environmental microbiota

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