Phenotypic and Genotypic Antibiotic Resistance of Salmonella from Chicken Carcasses Marketed at Ibague, Colombia

Velez, Diego Cortes; Rodríguez,; García, Noel Verján

doi:10.1590/1806-9061-2016-0405

Cited by 21 publications

(9 citation statements)

References 31 publications

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“…Our findings of high resistance to trimethoprim/sulfamethoxazole are similar to the results obtained previously [ 50 ] for S. enterica serovars isolated in Egypt from broiler chickens and chicken carcasses, as well as for Salmonella isolates from chicken carcasses sold in Ibagué, Columbia [ 51 ].…”

Section: Discussionsupporting

confidence: 91%

“…Strain SZL 38 had two different genes associated with resistance to aminoglycosides localized in chromosomes. Previously, it has been reported that there are different variants of resistance to aminoglycosides in the same strains isolated from the poultry production chain [ 42 , 51 ]. This may be due to the intense selection pressure in these production lines.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Antibiotic Resistance of Salmonella Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia

et al. 2021

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Food products may be a source of Salmonella, one of the main causal agents of food poisoning, especially after the emergence of strains resistant to antimicrobial preparations. The present work dealt with investigation of the occurrence of resistance to antimicrobial preparations among S. enterica strains isolated from food. The isolates belonged to 11 serovars, among which Infantis (28%), Enteritidis (19%), and Typhimurium (13.4%) predominated. The isolates were most commonly resistant to trimethoprim/sulfamethoxazole (n = 19, 59.38%), cefazolin (n = 15, 46.86%), tetracycline (n = 13, 40.63%), and amikacin (n = 9, 28.13%). Most of the strains (68.75%) exhibited multiple resistance to commonly used antibiotics. High-throughput sequencing was used to analyse three multidrug-resistant strains (resistant to six or more antibiotics). Two of them (SZL 30 and SZL 31) belonged to S. Infantis, while one strain belonged to S. Typhimurium (SZL 38). Analysis of the genomes of the sequenced strains revealed the genes responsible for antibiotic resistance. In the genomes of strains SZL 30 and SZL 31 the genes of antibiotic resistance were shown to be localized mostly in integrons within plasmids, while most of the antibiotic resistance genes of strain SZL 38 were localized in a chromosomal island (17,949 nt). Genomes of the Salmonella strains SZL 30, SZL 31, and SZL 38 were shown to contain full-size pathogenicity islands: SPI-1, SPI-2, SPI-4, SPI-5, SPI-9, SPI-11, SPI-13, SPI-14, and CS54. Moreover, the genome of strain SZL 38 was also found to contain the full-size pathogenicity islands SPI-3, SPI-6, SPI-12, and SPI-16. The emergence of multidrug-resistant strains of various Salmonella serovars indicates that further research on the transmission pathways for these genetic determinants and monitoring of the distribution of these microorganisms are necessary.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Evaluation of Antibiotic Resistance of Salmonella Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia

et al. 2021

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“…In our study, 69.4% of the strains demonstrated the presence of the genes bla PSE-1 and bla TEM that encode beta-lactamases that confer resistance to AM. In a study conducted in Ibagué (Colombia), 100% of the strains isolated from chicken carcasses had the gene bla TEM [31], a frequency that was higher than that found in the present study. Some strains that were phenotypically resistant to AM and CRO showed the presence of the genes bla PSE-1 , bla TEM , bla CMY2 , and bla CTX-M .…”

Section: Discussioncontrasting

confidence: 87%

“…On the other hand, the gene strB also confers resistance to STR and encodes a phosphotransferase that catalyzes the ATP-dependent phosphorylation of hydroxyl groups in STR [ 43 ]. The strB gene was found in 87.8% of the strains, and similar results have been reported in Salmonella strains isolated from chicken carcasses in Ibagué [ 31 ]. Regarding the strains isolated from human subjects, it was observed that the strains contained three of four genes that conferred resistance to STR, whereas some strains were not resistant to this antibiotic.…”

Section: Discussionsupporting

confidence: 82%

Molecular characterization of antimicrobial resistance and enterobacterial repetitive intergenic consensus-PCR as a molecular typing tool for Salmonella spp. isolated from poultry and humans

2020

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Background and Aim: Salmonella spp. are one of the most important food-borne pathogens in the world, emerging as a major public health concern. Moreover, multidrug-resistant (MDR) strains have been isolated from salmonellosis outbreaks, which compromise its treatment success. This study was conducted to characterize the phenotypic and genotypic antibiotic resistance profile of Salmonella strains isolated from broilers and humans from the regions of Tolima and Santander (Colombia). Materials and Methods: Salmonella spp. strains (n=49) were confirmed through molecular detection by amplification of the invA gene. Phenotypic antibiotic resistance was determined by the automated method and the agar diffusion method, and the presence of resistance genes was evaluated by PCR. Genotypic characterization was conducted using the enterobacterial repetitive intergenic consensus (ERIC)-PCR method, from which a dendrogram was generated and the possible phylogenetic relationships were established. Results: Salmonella isolates were classified as MDR strains exhibiting resistance to four antibiotic classes, penicillins, aminoglycosides, sulfonamides, and cephalosporins, and the human strains were resistant to gentamicin. At the genotypic level, the isolates contained the genes blaCMY2, blaCTX-M, blaPSE-1, blaTEM, aadA1, srtB, dfrA1, sul2, and floR. The genotyping results obtained by ERIC-PCR allowed the grouping of strains according to the source of isolation. Conclusion: The Salmonella spp. strains exhibited resistance to multiple antibiotics, as well as multiple genes associated with them, and the ERIC-PCR method was a technique that was helpful in generating clusters with biological significance.

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“…The drugs that were least effective were ciprofloxacin, tobramycin, cephalothin and azithromycin. Several studies have evaluated resistance to tobramycin and cephalothin, finding very variable results ranging from 100% resistant isolates to less than 18% resistant isolates [34][35][36][37].…”

Section: Discussionmentioning

confidence: 99%

Microbiological assessment at slaughter of chicken carcasses from commercial, backyard and semi-backyard production systems

Garcia

Nascimento

Dias

et al. 2021

J Infect Dev Ctries

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Introduction: Smaller scale, alternative, chicken production systems are gaining popularity globally. However, this brings public health and market confidence concerns, especially where there are no established standards of production. The aim of this study was to carry out a microbiological analysis of chicken carcasses from the commercial, backyard and semi-backyard production systems, slaughtered in the same slaughterhouse. Methodology: Samples of 102 chicken carcasses were taken in two steps of the slaughter (A: after bleeding; and B: after chiller tank) and were subjected to aerobic mesophilic, coliforms at 35 °C and coliforms at 45 °C counts, and Salmonella spp. detection. Salmonella spp. isolates were subjected to antimicrobial resistance analysis. Results: At slaughter step A, carcasses from the backyard system had less contamination than carcasses from the commercial system, with a difference of 0.7 log10 CFU/mL. Salmonella was identified in carcasses of all production systems and in both slaughter steps. Nine chicken carcasses were positive for Salmonella and no significant difference was observed in the occurrence of Salmonella amongst the carcasses from different production systems. Two Salmonella isolates, that presented the highest resistance profiles (one isolate was resistant to eight and the other to six out of ten tested antibiotics), were identified on carcasses from the semi-backyard system. Conclusions: Carcasses from the backyard system had a lower microbial count at the initial step of the slaughter process than the commercial production system. In addition, greater resistance to antimicrobials was observed in Salmonella isolates from semi-backyard system.

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Phenotypic and Genotypic Antibiotic Resistance of Salmonella from Chicken Carcasses Marketed at Ibague, Colombia

Cited by 21 publications

References 31 publications

Evaluation of Antibiotic Resistance of Salmonella Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia

Evaluation of Antibiotic Resistance of Salmonella Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia

Molecular characterization of antimicrobial resistance and enterobacterial repetitive intergenic consensus-PCR as a molecular typing tool for Salmonella spp. isolated from poultry and humans

Microbiological assessment at slaughter of chicken carcasses from commercial, backyard and semi-backyard production systems

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