Phenotypic and genotypic analysis of Borrelia burgdorferi isolates from various sources

Adam, Thomas C.; Gassmann, G.; Rasiah, Christiane; Göbel, Ulf B.

doi:10.1128/iai.59.8.2579-2585.1991

Cited by 105 publications

(50 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Strains of B. burgdorferi sensu lato related to Lyme disease are highly heterogeneous in their gene structures, and the three genospecies, B. burgdorferi sensu stricto, B. garinii and B. afzelii, have been proposed on the bases of DNA homology (3,9,28), and 16S rRNA gene and fla restriction analyses (1). Japanese isolates were characterized bacteriologically based on protein profiles (25), plasmid profiles (32), restriction fragment length polymorphism (RFLP) analysis in 23S rRNA gene, 16S rRNA gene (12,13,15,19) and fla (19) and reactivities with MAbs against Osp A and flagellin (25).…”

mentioning

confidence: 99%

Polymerase Chain Reaction Analysis of Borrelia Species Isolated in Japan

Kawabata

Tashibu

Yamada

et al. 1994

Microbiology and Immunology

View full text Add to dashboard Cite

Primer reactivities of 25 Borrelia burgdorferi sensu lato isolates from the ticks, Ixodes persulcatus and I. ovatus, in Japan and 10 isolates in Europe and North America were investigated. The methods used in this study were the polymerase chain reaction (PCR) on the flagellin structural gene (fla), the outer surface protein A gene (osp A) and the outer surface protein B gene (osp B), and the restriction fragment length polymorphism (RFLP) analysis of PCR products from osp A and osp B. The flagellin PCR primer set reacted with all the Borrelia strains tested. Four genospecies, B. burgdorferi sensu stricto, B. garinii, B. afzelii and B. japonica, were differentiated by PCR using osp A and osp B primers combined with RFLP analysis. Some Japanese isolates from I. persulcatus were identified as B. garinii or B. afzelii. The other isolates from I. persulcatus did not fit in any of the 4 genospecies.These results suggested that Japanese isolates from I. persulcatus are highly heterogeneous in their osp A and osp B structures. Furthermore, PCR primers targetingfla are applicable to the gene diagnosis for Lyme disease in Japan, and osp A and osp B primers can be used to classify B. burgdorferi sensu lato isolates into genospecies by PCR and RFLP analyses.

show abstract

mentioning

confidence: 99%

Polymerase Chain Reaction Analysis of Borrelia Species Isolated in Japan

Kawabata

Tashibu

Yamada

et al. 1994

Microbiology and Immunology

View full text Add to dashboard Cite

show abstract

“…Genetic, biochemical and immunological studies revealed significant differences among the Lyme disease borreliae isolated from various biological and geographical sources (1,25,27,28,40). Previous reports (13)(14)(15), and our recent studies on the basis of the reactivities with 16 monoclonal antibodies to outer surface protein A and DNA-DNA hybridization indicated that B. garinii strains isolated in Japan are heterogeneous and different from B. garinii isolates in Europe (33).…”

Section: Discussionmentioning

confidence: 82%

“…To date, the causative agent of the disease has been found to consist of at least three genospecies that are human pathogens; Borrelia burgdorferi sensu stricto (s.s.), Borrelia garinii and Borrelia afzelii. Some differences in disease manifestation are associated with infection by each of these genospecies (1,2,8).…”

mentioning

confidence: 99%

Consensus Sequence on the Genes Encoding the Major Outer Surface Proteins (OspA and OspB) of Borrelia garinii Isolate

Wang

Masuzawa

Komikado

et al. 1997

Microbiology and Immunology

View full text Add to dashboard Cite

Japanese Lyme borrelias classified as ribotype IV is predominant among isolates derived from clinical specimens, reservoir rodents and Ixodes persulcatus ticks, and has been characterized as Borrelia garinii. These B. garinii isolates have antigenic and genetic features apparently different from North American, European and other Asian isolates, especially in major outer surface proteins A (OspA) and B (OspB). In this study, we cloned and sequenced the genes encoding OspA and OspB from B. garinii strain FujiP2 (ribotype IV strain) isolated from I. persulcatus in Shizuoka, Japan. A sequence analysis revealed significant differences to the previously published sequences of ospA and ospB of B. burgdorferi sensu lato. The open reading frames of ospA and ospB consist of 822 and 888 nucleotides corresponding to the proteins of 273 and 295 amino acids, with molecular weights of 29,643 and 31,786 daltons, respectively. The most interesting finding is that the two osp genes share a consensus 282 by sequence in their carboxy-terminal portions and that the ospB gene is flanked by a 282 bp-long direct repeat sequence. The deduced amino-acid (aa) sequences of OspA and OspB of strain FujiP2 showed 60.1% homology, and have overall similarities of 70.5%, 703% and 75.6% to OspAB proteins of B. burgdorferi sensu stricto strain B31, Borrelia afzelii strain ACA1 and Borrelia garinii strain Ip90, respectively.

show abstract

“…A number of Borrelia isolates have been obtained from diverse geographic locations. Many Borrelia isolates have been examined by monoclonal antibody (1,4,5), plasmid profile analysis (6,17,18,30), and DNA restriction pattern analysis (18,22). Recently, on the basis of DNA homology, specific rRNA gene restriction patterns, and protein electrophoresis patterns, three genospecies for the strains of B. burgdorferi were proposed (2).…”

Section: Discussionmentioning

confidence: 99%