Phenotypic and genetic diversity of chlorine-resistant Methylobacterium strains isolated from various environments

Hiraishi, Akira; Furuhata, Katsunori; Matsumoto, Atsuhiko; Koike, Kazuko; Fukuyama, Masafumi; Tabuchi, Ken

doi:10.1128/aem.61.6.2099-2107.1995

Cited by 121 publications

(55 citation statements)

References 47 publications

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“…In the phylogenetic analysis, since the strains belonging to Group B formed 74.1%, it became clear that there are many strains belonging to Group B in tap water, and it was in agreement with results reported by Hiraishi et al (11). These isolates were investigated with regard to the relationship with the isolated areas and the residual chlorine concentration in tap water, but no marked tendencies were noted in geographical differences or with regard to chlorine resistance.…”

Section: Discussionsupporting

confidence: 87%

“…The objective of this study was to elucidate which species of Methylobacterium inhabit tap water in hospitals. Since Methylobacterium species were isolated from various samples, including aqueous environments (11,15), it was showed that the bacteria were widely distributed in natural environments. Because of the diversity in the properties among the strains, new species have been reported continually since 2000 (1,8,13).…”

Section: Discussionmentioning

confidence: 99%

“…Consequently, there has been a move to save on the costs for infection control by removing equipment installed for sterilized water and switching to the use of tap water. Although the chlorine sterilization of tap water is mandatory in Japan, Methylobacterium species resistant to the residual chlorine of disinfectants widely inhabit tap water piped through water supply facilities such as water tanks of high-rise buildings (7,11). In 1990, Furuhata et al (6) isolated Methylobacteritum strains from hospital tap water in high frequency, showing that hospital tap water is not free from the contamination.…”

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confidence: 99%

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Isolation and Identification of Methylobacterium Species from the Tap Water in Hospitals in Japan and Their Antibiotic Susceptibility

Furuhata

Kato²,

Goto³

et al. 2006

Microbiology and Immunology

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Contamination of tap water by Methylobacterium species has become a serious concern in hospitals. This study was planned to examine the distribution of Methylobacterium species inhabiting tap water used in Japanese hospitals and antibiotic sensitivity of the isolates in 2004. Species identification of 58 isolates was performed based on the homology of a partial sequence of 16S rDNA. The dominant Methylobacterium species in hospital water were M. aquaticum and M. fujisawaense. To examine the biochemical properties of these isolates, a carbon source utilization was tested using an API50CH kit. The phenotypic character varied widely, and was not necessarily consistent with the results of phylogenic analysis based on the partial 16S rDNA sequence, suggesting that the biochemical properties are not suitable for identification of Methylobacterium species. The isolates were also subjected to antibiotic sensitivity tests. They were resistant to 8 antibiotics, but highly sensitive to imipenem (MIC90=1 μg/ml) and tetracycline (MIC90=8 μg/ml). These findings concerning the isolates revealed the presence of Methylobacterium species with resistance to multiple antibiotics in hospital tap water.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Isolation and Identification of Methylobacterium Species from the Tap Water in Hospitals in Japan and Their Antibiotic Susceptibility

Furuhata

Kato²,

Goto³

et al. 2006

Microbiology and Immunology

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“…Methylobacterium is frequently found in oligotrophic environments, such as water reservoirs and drinking water vessels as well as clean habitats (La Duc et al, 2007). Their resistance to high levels of chlorine has been described (Hiraishi et al, 1995), and it is possible that cleaning agents used in these assembly Table 4. Sequences of bacterial species retrieved from the surrounding air of various spacecraft assembly facilities…”

Section: Discussionmentioning

confidence: 99%

Molecular bacterial community analysis of clean rooms where spacecraft are assembled

Moissl

Osman

Duc

et al. 2007

FEMS Microbiology Ecology

116

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Molecular bacterial community composition was characterized from three geographically distinct spacecraft-associated clean rooms to determine whether such populations are influenced by the surrounding environment or the maintenance of the clean rooms. Samples were collected from facilities at the Jet Propulsion Laboratory (JPL), Kennedy Space Flight Center (KSC), and Johnson Space Center (JSC). Nine clone libraries representing different surfaces within the spacecraft facilities and three libraries from the surrounding air were created. Despite the highly desiccated, nutrient-bare conditions within these clean rooms, a broad diversity of bacteria was detected, covering all the main bacterial phyla. Furthermore, the bacterial communities were significantly different from each other, revealing only a small subset of microorganisms common to all locations (e.g. Sphingomonas, Staphylococcus). Samples from JSC assembly room surfaces showed the greatest diversity of bacteria, particularly within the Alpha- and Gammaproteobacteria and Actinobacteria. The bacterial community structure of KSC assembly surfaces revealed a high presence of proteobacterial groups, whereas the surface samples collected from the JPL assembly facility showed a predominance of Firmicutes. Our study presents the first extended molecular survey and comparison of NASA spacecraft assembly facilities, and provides new insights into the bacterial diversity of clean room environments .

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“…For example, the finished water sample collected on 8 September 2017 contained a high relative abundance of Stramenopiles (67% of the total operational taxonomic units (OTUs), table S12), which is commonly associated with algae [31]. A surge in Methylobacterium was also noted in samples taken from City B's distribution system on the first two sampling dates (table S12); this phylum is often associated with soil enviornments [32]. This surge in plant-and soil-associated bacteria coincides with a surge in TOC in City B's raw and finished waters.…”

Section: City Bmentioning

confidence: 99%

Impacts of Hurricane Harvey on drinking water quality in two Texas cities

Landsman

Rowles

Brodfuehrer

et al. 2019

Environ. Res. Lett.

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Hurricane Harvey devastated large parts of the US Gulf Coast in 2017, and its floodwaters posed a number of threats to the environment and human health. In particular, an estimated 375 000 Texas residents experienced issues related to the provision of safe drinking water at the peak of the hurricane. In this study, physical, chemical, and biological water quality was monitored in two drinking water systems in Texas following Hurricane Harvey to understand the relationship between water quality parameters and changes in the drinking water microbiota. Results show initial surges in total organic carbon, trihalomethanes, and bacterial concentrations in finished water immediately following Hurricane Harvey. Microbial community analyses highlight the dependence of the distribution system microbiota on distribution system characteristics (i.e. water age), raw water quality, and disinfectant residual, among other factors. While both systems had problems maintaining disinfectant residual in the weeks following the hurricane, stabilization of water quality occurred over time.Overall, this study provides an understanding of the challenges associated with maintaining drinking water quality in the wake of a natural disaster and can be used to better prepare drinking water managers and engineers to combat changing weather patterns in the future.

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Phenotypic and genetic diversity of chlorine-resistant Methylobacterium strains isolated from various environments

Cited by 121 publications

References 47 publications

Isolation and Identification of Methylobacterium Species from the Tap Water in Hospitals in Japan and Their Antibiotic Susceptibility

Isolation and Identification of Methylobacterium Species from the Tap Water in Hospitals in Japan and Their Antibiotic Susceptibility

Molecular bacterial community analysis of clean rooms where spacecraft are assembled

Impacts of Hurricane Harvey on drinking water quality in two Texas cities

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