Phenotypic and Genetic Characterization of Flavobacterium psychrophilum Recovered from Diseased Salmonids in China

Li, Shaowu; Chai, Jingru; Knupp, Christopher; Nicolas, Pierre; Wang, Di; Cao, Yanghui; Deng, Furong; Chen, Fuguang; Lü, Tao; Loch, Thomas P.

doi:10.1128/spectrum.00330-21

Cited by 15 publications

(21 citation statements)

References 56 publications

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“…bacterial fitness, virulence, colonization of a specific host). Anyhow, this situation is in sharp contrast with the conclusions obtained with F. psychrophilum where obvious links between the serotype and the host fish species were noticed (Li et al., 2021; Rochat et al., 2017). Strikingly, in T. maritimum the Types distribution does not follow the core genome‐based phylogeny (Figure 4).…”

Section: Discussioncontrasting

confidence: 63%

Genomic characterization of Tenacibaculum maritimum O‐antigen gene cluster and development of a multiplex PCR‐based serotyping scheme

López

Bridel

Saulnier

et al. 2022

Transbounding Emerging Dis

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Tenacibaculum maritimum is a devastating bacterial pathogen affecting a large variety of marine fish species. It is responsible for significant economic losses in aquaculture farms worldwide. Different typing methods have been proposed to analyse bacterial diversity and population structure. Serological heterogeneity has been observed and up to four different serotypes have been described so far. However, the underlying molecular factors remain unknown. By combining conventional serotyping and genome‐wide association study, we identified the genomic loci likely involved in the O‐antigen biosynthesis. This finding allowed the development of a robust multiplex PCR‐based serotyping scheme able to detect subgroups within each serotype and therefore performs better than conventional serotyping. This scheme was successfully applied to a large number of isolates from worldwide origin and retrieved from a large variety of fish species. No obvious correlations were observed between the mPCR‐based serotype and the host species or the geographic origin of the isolates. Strikingly, the distribution of mPCR‐based serotypes does not follow the core genome phylogeny. Nevertheless, this simple and cost‐effective mPCR‐based serotyping method could be useful for different applications such as population structure analysis, disease surveillance, vaccine formulation and efficacy follow‐up.

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Section: Discussioncontrasting

confidence: 63%

Genomic characterization of Tenacibaculum maritimum O‐antigen gene cluster and development of a multiplex PCR‐based serotyping scheme

López

Bridel

Saulnier

et al. 2022

Transbounding Emerging Dis

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“…The F. psychrophilum isolate CN06, which belongs to ST12 within CC-ST10 ( 2 ), originally recovered from the ulcerated muscle of diseased rainbow trout and previously confirmed to be virulent in in vivo challenge experiments, was selected for use in the present study. The CN06 strain was grown for 72 h in the TYES broth medium at 15°C, then collected by centrifuging at 6,000 × g for 5 min and resuspended to a concentration of 10 8 Colony-Forming Units (CFU)·ml −1 in sterile PBS (pH 7.2).…”

Section: Methodsmentioning

confidence: 99%

“…To detect F. psychrophilum in the spleen of infected rainbow trout, a PCR assay was conducted following the procedures described by Li et al. ( 2 ).…”

Section: Methodsmentioning

confidence: 99%

“…The CN06 strain was grown for 72 h in the TYES broth medium at 15°C, then collected by centrifuging at 6,000 × g for 5 min and resuspended to a concentration of 10 8 Colony-Forming Units (CFU)•ml −1 in sterile PBS (pH 7.2). To detect F. psychrophilum in the spleen of infected rainbow trout, a PCR assay was conducted following the procedures described by Li et al (2).…”

Section: Bacterial Strain Isolation and Culturementioning

confidence: 99%

“…The outbreaks of bacterial coldwater disease (BCWD) and rainbow trout fry syndrome continue to cause considerable losses in freshwater salmonid aquaculture around the globe ( 1 ). The etiological agent of this disease, Flavobacterium psychrophilum , is a Gram-negative rod-shaped bacterium ( 2 ) that has been reported throughout Asia, Europe, North America, South America, and Australia ( 3 ). Although infections have been reported in other fish species, a wide variety of salmonid species are susceptible during freshwater stages ( 4 ).…”

Section: Introductionmentioning

confidence: 99%

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Time-course transcriptome analyses of spleen in rainbow trout (Oncorhynchus mykiss) post-Flavobacterium psychrophilum infection

et al. 2022

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Flavobacterium psychrophilum, the etiological agent of bacterial coldwater disease and rainbow trout fry syndrome, causes considerable losses in salmonid aquaculture globally. Systemic F. psychrophilum infections in rainbow trout (Oncorhynchus mykiss) lead to a range of clinical signs, including ulcerative lesions in the skin and muscle and splenitis. Previous studies offered an integrative analysis of the skeletal muscle response to F. psychrophilum infection in rainbow trout. However, little is known about the molecular mechanism of immune response in the spleen, which is an important immune organ of rainbow trout. Here, we investigated the time-course splenic transcriptome profiles in uninfected rainbow trout (CK) and F. psychrophilum–infected rainbow trout at day 3 and day 7 (D3, D7) by RNA-seq analyses. Among the 7,170 differentially expressed genes (DEGs) in the three comparisons (D3 vs. CK, D7 vs. CK, D3 vs. D7), 1,286 DEGs showed consistent upregulation or downregulation at D3 and D7 and were associated with pattern recognition, acute-phase response, complement cascade, chemokine and cytokine signaling, and apoptosis. The Real time quantitative PCR (RT-qPCR) analysis of eight DEGs confirmed the accuracy of the RNA-Sequencing (RNA-seq) data. Our results reflected a general process from pathogen recognition to inflammatory cytokine generation and delineated a putative Toll-like receptor signaling pathway in rainbow trout spleen, following F. psychrophilum infection. Taken together, these results provide new insights into the molecular mechanism of the immune response to F. psychrophilum infection and are a valuable resource for future research on the prevention and control of bacterial coldwater disease during salmon culture.

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Bacteriophages in the fish mucosa: updates on their biology, interactions, and applications

Leonora,

Boonyarat,

Pugoy

et al. 2024

Biologia

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Phenotypic and Genetic Characterization of Flavobacterium psychrophilum Recovered from Diseased Salmonids in China

Abstract: Flavobacterium psychrophilum causes substantial economic losses in salmonid aquaculture worldwide. Although this bacterium is also believed to be a disease source in China, published reports of its presence do not yet exist.

Cited by 15 publications

References 56 publications

Genomic characterization of Tenacibaculum maritimum O‐antigen gene cluster and development of a multiplex PCR‐based serotyping scheme

Genomic characterization of Tenacibaculum maritimum O‐antigen gene cluster and development of a multiplex PCR‐based serotyping scheme

Time-course transcriptome analyses of spleen in rainbow trout (Oncorhynchus mykiss) post-Flavobacterium psychrophilum infection

Bacteriophages in the fish mucosa: updates on their biology, interactions, and applications

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