Phenotypic and functional differentiation of porcine αβ T cells: Current knowledge and available tools

Gerner, Wilhelm; Talker, Stephanie C.; Koinig, Hanna C.; Sedlak, Corinna; Mair, Kerstin H.; Saalmüller, Armin

doi:10.1016/j.molimm.2014.10.025

Cited by 81 publications

(96 citation statements)

References 100 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, especially in the field of T cell biology, there has been remarkable progress in developing pig-specific and cross-reactive antibodies for multicolor flow cytometry (8, 10–14), in diagnostic bead-based multiplex assays (15) as well as in annotating the porcine immune system (16). These efforts helped to expand the opportunities for studying adaptive immunity in this human-relevant large animal model.…”

Section: Introductionmentioning

confidence: 99%

“…Pigs are natural hosts for several important zoonotic pathogens infecting humans alike [e.g., Streptococcus suis, Ascaris suum , and Toxoplasma gondii (35–37)]. In addition, pigs exhibit very similar pathologies (3, 8) and are of importance for vaccine development for pigs and human likewise. Hence, tools for an extended multiparameter analysis of rare pathogen-specific T cells are of great importance.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pathogen-Reactive T Helper Cell Analysis in the Pig

et al. 2017

View full text Add to dashboard Cite

There is growing interest in studying host–pathogen interactions in human-relevant large animal models such as the pig. Despite the progress in developing immunological reagents for porcine T cell research, there is an urgent need to directly assess pathogen-specific T cells—an extremely rare population of cells, but of upmost importance in orchestrating the host immune response to a given pathogen. Here, we established that the activation marker CD154 (CD40L), known from human and mouse studies, identifies also porcine antigen-reactive CD4+ T lymphocytes. CD154 expression was upregulated early after antigen encounter and CD4+CD154+ antigen-reactive T cells coexpressed cytokines. Antigen-induced expansion and autologous restimulation enabled a time- and dose-resolved analysis of CD154 regulation and a significantly increased resolution in phenotypic profiling of antigen-responsive cells. CD154 expression identified T cells responding to staphylococcal Enterotoxin B superantigen stimulation as well as T cells responding to the fungus Candida albicans and T cells specific for a highly prevalent intestinal parasite, the nematode Ascaris suum during acute and trickle infection. Antigen-reactive T cells were further detected after immunization of pigs with a single recombinant bacterial antigen of Streptococcus suis only. Thus, our study offers new ways to study antigen-specific T lymphocytes in the pig and their contribution to host–pathogen interactions.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pathogen-Reactive T Helper Cell Analysis in the Pig

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Discussion In comparison to the progress that has been made in the characterization of camel immunoglobulins (HamersCasterman et al, 1993;Muyldermans, 2013), few data are available on cellular immunity of camels in health or disease. MAbs to leukocyte antigens have been considered as highly important tools for the analysis of cellular immunology in human (Maecker et al, 2012) as well as in different animal species Lunn et al, 1998;Schafer and Burger, 2012;Duvel et al, 2014;Gerner et al, 2015). The lack of mAbs that define camel immune cells represents one of the major difficulties in studying the camel cellular immune response.…”

Section: Reactivity Of Mabs To Human Leukocyte Antigens With Camel Grmentioning

confidence: 99%

“…In comparison to several other veterinary species like cattle (Hussen et al, 2013;Duvel et al, 2014), pigs (Gerner et al, 2015), sheep and horses (Lunn et al, 1998), the immune system of camels remains to a great extent poorly studied. Although considerable progress has been made in the characterization of camel immunoglobulins (HamersCasterman et al, 1993;Muyldermans, 2013), few data are available on the cellular immunity of camels in health or disease (Zidan et al, 2000a,b;Al-Mohammed Salem et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Reactivity of commercially available monoclonal antibodies to human CD antigens with peripheral blood leucocytes of dromedary camels (<i>Camelus dromedarius</i>)

Hussen¹,

Shawaf²,

Al-herz³

et al. 2017

Open Vet J.

View full text Add to dashboard Cite

Monoclonal antibodies (mAbs) to cell surface molecules have been proven as a key tool for phenotypic and functional characterization of the cellular immune response. One of the major difficulties in studying camel cellular immunity consists in the lack of mAbs that dtect their leukocyte differentiation antigens. In the present study two-parameter flow cytometry was used to screen existing commercially available mAbs to human leukocyte antigens and major histocompatibility molecules (MHC) for their reactivity with camel leukocytes. The comparison of patterns of reactivity obtained after labelling human and camel leukocytes have shown that mAbs specific to human cluster of differentiation (CD) 18, CD11a, CD11b and CD14 are predicted to be cross-reactive with homologous camel antigens.

show abstract

“…PE-conjugated MAb against porcine CD8a (clone 76-2-11, BD Pharmingen) and FITC-conjugated MAb against porcine CD3" (clone PPT3, Southern Biolegend) were additionally used to characterize cells that stained positive with the tetramers. CD3 + CD8 high cells contain a CD8 + ab T-cell subpopulation which recognises class-I epitopes [5,15] high ranged from 0.4 to 2.6 %, depending on individual peptides and animals ( Table 2). Nine peptides were identified as T-cell epitopes by tetramer staining; three located in the M1 protein (Tet62, Tet56 and Tet64), three in NA (Tet55, Tet60 and Tet59) and three in HA (Tet93, Tet57 and Tet66).…”

mentioning

confidence: 99%