Phenotypic and functional characterization of intestinal epithelial exosomes

Mallegol, Julia; Niel, Guillaume van; Heyman, Martine

doi:10.1016/j.bcmd.2005.04.001

Cited by 74 publications

(55 citation statements)

References 20 publications

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“…There are still many puzzles of ovarian cancer, including the relationship between the tumor and the host immune system, and the mechanisms of the development, invasion and metastasis of the tumor. Exosomes are a kind of microvesicles released by many types of cells such as tumor cell (1)(2)(3)(4), antigen presenting cells (APC) (5,6) and epithelial cells (7,8). Recent research revealed that exosomes purified from the ascites of patient with ovarian cancer contain some immunological-effect molecules (9).…”

Section: Introductionmentioning

confidence: 99%

Exosomes in the ascites of ovarian cancer patients: origin and effects on anti-tumor immunity

2011

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Abstract. This study was performed to identify the origin of the ascites-derived exosomes from patients with ovarian cancer and to observe the effect of exosomes on anti-tumor immunity. Exosomes were isolated from the ascites of patients with ovarian epithelial cancer by ultracentrifugation plus density gradient centrifugation. The origin of exosomes was identified by immunoelectronmicroscopy (IEM). The growth curve of the tumor cell line SKOV3 cultured with or without exosomes was analyzed. The apoptosis of autogeneic tumor cells (ATCs) and SKOV3 cells affected by exosomes was measured by flow cytometry (FCM) and light phase contrast microscopy. The cytotoxic effect of the peripheral blood mononuclear cells (PBMCs) stimulated by exosomes and/or dendritic cells (DCs) on ovarian cancer cells was measured using a CCK-8 assay. The levels of IFN-Á released by PBMCs stimulated by exosomes and/or DCs were measured by ELISA. The apoptosis of PBMCs and DCs affected by exosomes was measured by FCM and light microscopy. Whether the mature process of DCs was affected by exosomes was studied by FCM. The ratio of CD4 + T cell and CD8 + T cell were measured by FCM. FasL and TRAIL molecules on exosomes were detected by Western blot analysis. The human FasL antagonistic antibody was used to block the apoptosis of DCs and PBMCs induced by exosomes. The receptors of TRAIL DR4 and DR5 on PBMCs and DCs were detected by FCM. In 41 patients examined, we isolated exosomes from the ascites of 35 patients. We detected TCR, CD20, HLA-DR, B7-2, HER2/neu, CA125 and Histone H 2 A on exosomes. We found that exosomes might impair the cytotoxic activity of PBMCs when DCs are present. We found that exosomes had no effect on the growth and apoptosis of SKOV3 cells. However, exosomes may induce apoptosis of precursors, mature DCs and PBMCs. We found that FasL and TRAIL were present in the exosome suspension and addition of an anti-FasL antibody may decrease the percentage of apoptosis of DCs and PBMCs. We conclude that exosomes exist in ascites of 85.4% of patients with ovarian cancer. Moreover, these exosomes may be of multi-origin. Exosomes had no effect on the growth and apoptosis of tumor cells but impaired the cytotoxic activity of PBMCs in the presence of DCs. Exosomes also may induce apoptosis of the precursors of DCs, DCs and PBMCs. FasL and TRAIL on exosomes may partly account for the apoptosis of cells of the immune system.

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Section: Introductionmentioning

confidence: 99%

Exosomes in the ascites of ovarian cancer patients: origin and effects on anti-tumor immunity

2011

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“…Although the intestinal mucosa and lungs are not considered to be immune organs, they expressed MDIi-2 at relatively high levels. This may be because the mucosa membrane of these 2 organs is located at a strategic position between the external environment and the most extended lymphoid tissue in organs that resist pathogens from the external environment (Mallegol et al, 2005).…”

Section: Conservation Of Mdii-2 Indicates the Same Function And Similmentioning

confidence: 99%

Molecular characterization and tissue-specific expression of invariant chain isoform in Muscovy Duck (Cairina moschata)

Liu¹,

Chen²,

Wu³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Invariant chain (Ii) isoform, through its thyroglobulin-like (Tg) domain, inhibits cysteine proteases during antigen presentation in vertebrates. In birds, the Ii of Muscovy Duck (MDIi) has 2 forms: MDIi-1 and MDIi-2 (MDIi isoform). To understand the genetic information and expression characteristics of MDIi-2, polymerase chain reaction, and bioinformatic analysis were performed for MDIi-2 from healthy adult Muscovy Duck. The full-length MDIi-2 cDNA sequence was found to be 1377-base pairs, encoding a 285-amino acid protein. MDIi-2 contains 63 amino acids with an insertion sequence in the Tg domain. MDIi-2 shares high identity (72.51-94.74%) with the same protein in other birds. The Tg domain of MDIi-2 is highly conserved and showed relatively high identity (96.83%) among all tested birds. The molecular structure of the Tg domain supports this conservation. MDIi-2 expression was measured in various tissues using real-time quantitative polymerase chain reaction. Similar to MDIi-1, MDIi-2 was detected in all tissues but at different levels. Higher expression level was observed in the spleen, intestinal mucosa, and bursa stipe (bursa of Fabricius stipe) than in other tissues. This suggests that MDIi-2, like MDIi-1, plays an essential role in all tissues and that its differential expression may be related to its functions in these tissues. The coexistence of 2 MDIi isoforms indicates that their functions are correlated in Muscovy Duck. This study improves the understanding of poultry immunology and may be used to improve measures to protect Muscovy Duck from disease.

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“…Zhi-Ming Liu 1& , Yu-Bin Wang 2& , Xian-Hou Yuan 1 * (Mallegol et al, 2005;Hendrix et al, 2011;Martin-Jaular et al, 2011). Types of tumor cells such as ovarian cancer, chronic myelogenous leukemia were demonstrated to secret functional exosomes (Cho et al, 2011;Clayton et al, 2011;Hood et al, 2011;Taverna et al, 2012).…”

Section: Exosomes From Murine-derived Gl26 Cells Promote Glioblastomamentioning

confidence: 99%

Exosomes from Murine-derived GL26 Cells Promote Glioblastoma Tumor Growth by Reducing Number and Function of CD8+T Cells

Liu¹,

Wang²,

Yuan³

2013

Asian Pacific Journal of Cancer Prevention

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Aim: Brain tumors almost universally have fatal outcomes; new therapeutics are desperately needed and will only come from improved understandins of glioma biology. Methods: Exosomes are endosomally derived 30~100 nm membranous vesicles released from many cell types. Examples from GL26 cells were here purified using density gradient ultracentrifugation and monitored for effects on GL26 tumor growth in C57BL/6j mice (H-2b). Lactate dehydrogenase release assays were used to detect the cytotoxic activity of CD8+T and NK cells.

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Phenotypic and functional characterization of intestinal epithelial exosomes

Cited by 74 publications

References 20 publications

Exosomes in the ascites of ovarian cancer patients: origin and effects on anti-tumor immunity

Exosomes in the ascites of ovarian cancer patients: origin and effects on anti-tumor immunity

Molecular characterization and tissue-specific expression of invariant chain isoform in Muscovy Duck (Cairina moschata)

Exosomes from Murine-derived GL26 Cells Promote Glioblastoma Tumor Growth by Reducing Number and Function of CD8+T Cells

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