Phenotypic and Functional Analysis of Tumour-lnfiltrating Lymphocytes from Patients with Renal Cell Carcinoma

Thiounn, Nicolas; Mathiot, Claire; Tartour, Éric; Joyeux, Isabelle; Peyret, C; Brandely, M.; Deslignières, S; Debré, B; Fridman, Wolf H.

doi:10.1159/000475271

Cited by 2 publications

(2 citation statements)

References 11 publications

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“…Tumor samples were obtained from 13 patients during surgery for their primary renal-cell carcinoma (RCC). TIL were obtained from tumors by enzymatic digestion as described (Thiounn et al, 1994). In some experiments, T lymphocytes from TIL day-0 preparations were enriched by a Ficoll-Hypaque gradient followed by incubation with anti-CD56 and anti-CD14 MAbs (Becton Dickinson, Mountain View, CA) and negative selection using microbeads conjugated with anti-mouse IgG ( I 0 bead Immunotech, Marseille, France).…”

Section: Isolation and Culture Of Tilmentioning

confidence: 99%

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Variable expression of CD3‐ζ chain in tumor‐infiltrating lymphocytes (TIL) derived from renal‐cell carcinoma: Relationship with til phenotype and function

Tartour

Latour

Mathiot

et al. 1995

Intl Journal of Cancer

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101

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It has been reported that in mice and in humans, tumor-infiltrating lymphocytes (TIL) may exhibit a defect in CD3-zeta-chain expression. Therefore, the level of CD3-zeta was analyzed in TIL derived from patients with renal-cell carcinoma, and its correlation with TIL phenotype and function was assessed. We identified 4 out of 13 tumor-infiltrating lymphocytes derived from renal-cell carcinoma, with a significant decrease in CD3-zeta-chain expression as compared with control peripheral-blood lymphocytes. This defect was never found after culturing TIL with IL2. In one case, the low expression of zeta chain observed in TIL on day 0 was reversed when TIL were cultured with IL2. The zeta-chain level did not seem to predict the growth of TIL in response to IL2. All the TIL, irrespective of the level of zeta-chain expression, exhibited lower proliferative response when stimulated with PHA or anti-CD3 MAb in comparison with normal peripheral-blood mononuclear cells. Nevertheless, in this limited series of patients, a correlation was observed between the level of zeta-chain expression and T-cell infiltration (p = 0.02). After TIL stimulation with PHA or anti-CD3, in contrast to IL2 or IFN-gamma production, a trend towards a relationship between TNF alpha induction and the level of zeta-chain expression was observed.

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Section: Isolation and Culture Of Tilmentioning

confidence: 99%

“…Unseparated TIL were expanded in vitro in AIM V medium (GIBCO BRL, Les Ulis, France) containing 1000 IU/ml of rIL2 and 20% lymphokine-activated killer supernatant (Thiounn et al, 1994). In all experiments, a pool of PBMC from normal patients was used as control.…”

Section: Isolation and Culture Of Tilmentioning

confidence: 99%