Phenotypic Analysis of Rodent Malaria Parasite Asexual and Sexual Blood Stages and Mosquito Stages

Aly, A. S.; Deveci, Gözde; Yilmaz, Ilknur; Abraham, Amanah; Golshan, Aneesa; Hart, Robert J.

doi:10.3791/55688

Cited by 13 publications

(11 citation statements)

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“…The non-lethal P. vinckei lentum strain and the Pyp230p(–) GFP expressing P. yoelii 17X-NL strain were stored as frozen stocks in liquid nitrogen [14,15]. Freshly thawed parasites were injected into donor naive mice, and a few days later 10,000 infected red blood cells (iRBCs) from donor mice were intravenously injected into the recipient experimental mice [16].…”

Section: Methodsmentioning

confidence: 99%

Highly Sensitive and Rapid Characterization of the Development of Synchronized Blood Stage Malaria Parasites Via Magneto-Optical Hemozoin Quantification

et al. 2019

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The rotating-crystal magneto-optical diagnostic (RMOD) technique was developed as a sensitive and rapid platform for malaria diagnosis. Herein, we report a detailed in vivo assessment of the synchronized Plasmodium vinckei lentum strain blood-stage infections by the RMOD method and comparing the results to the unsynchronized Plasmodium yoelii 17X-NL (non-lethal) infections. Furthermore, we assess the hemozoin production and clearance dynamics in chloroquine-treated compared to untreated self-resolving infections by RMOD. The findings of the study suggest that the RMOD signal is directly proportional to the hemozoin content and closely follows the actual parasitemia level. The lack of long-term accumulation of hemozoin in peripheral blood implies a dynamic equilibrium between the hemozoin production rate of the parasites and the immune system’s clearing mechanism. Using parasites with synchronous blood stage cycle, which resemble human malaria parasite infections with Plasmodium falciparum and Plasmodium vivax, we are demonstrating that the RMOD detects both hemozoin production and clearance rates with high sensitivity and temporal resolution. Thus, RMOD technique offers a quantitative tool to follow the maturation of the malaria parasites even on sub-cycle timescales.

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Section: Methodsmentioning

confidence: 99%

Highly Sensitive and Rapid Characterization of the Development of Synchronized Blood Stage Malaria Parasites Via Magneto-Optical Hemozoin Quantification

et al. 2019

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“…P. yoelii 17X-NL strain was stored as frozen stocks at −150 • C. Freshly thawed parasites were injected into donor-naive mice for mosquito feeding to obtain sporozoites. Anopheles stephensi mosquitos were infected by allowing them to feed on parasite-infected female BALB/c mice as described previously [7].…”

Section: Animals and Parasitesmentioning

confidence: 99%

“…Mice were intravenously challenged with P. yoelii 17X-NL salivary gland sporozoites resuspended in incomplete RPMI media. Sporozoites were prepared as described earlier [7]. Twelve weeks after the final immunization, each mouse was injected via the tail vein with 150 µL of incomplete RPMI media, containing 500 salivary gland sporozoites.…”

Section: Parasite Challengementioning

confidence: 99%

An Attenuated HSV-1-Derived Malaria Vaccine Expressing Liver-Stage Exported Proteins Induces Sterilizing Protection against Infectious Sporozoite Challenge

et al. 2022

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Here, we present the construction of an attenuated herpes simplex virus type-1 (HSV-1)-vectored vaccine, expressing three liver-stage (LS) malaria parasite exported proteins (EXP1, UIS3 and TMP21) as fusion proteins with the VP26 viral capsid protein. Intramuscular and subcutaneous immunizations of mice with a pooled vaccine, composed of the three attenuated virus strains expressing each LS antigen, induced sterile protection against the intravenous challenge of Plasmodium yoelii 17X-NL salivary gland sporozoites. Our data suggest that this malaria vaccine may be effective in preventing malaria parasite infection using practical routes of immunization in humans.

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“…All animal handling was conducted according to the approved protocols of the Institutional Animal Care and Use Committee (IACUC) of Tulane University (Protocol 4258R). Mosquito feeding experiments were conducted with P. yoelii 17X-NL wild-type parasites as previously described [53][54][55]. Briefly, SW mice, treated with phenylhydrazine, are injected intravenously with 1 million blood stage parasites, and on day 3 post infection an exflagellation assay is used to confirm the availability and formation of P. yoelii male microgametes.…”

Section: Mosquito Feeding and Sporozoite Extractionmentioning

confidence: 99%

Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from Plasmodium Challenge in a Mouse Model

et al. 2020

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tr (A.S.I.A.) † These authors contributed equally to this work and are presented in alphabetical order.Abstract: The need for a malaria vaccine is indisputable. A single vaccine for Plasmodium pre-erythrocytic stages targeting the major sporozoite antigen circumsporozoite protein (CSP) has had partial success. Additionally, CD8+ T cells targeting liver-stage (LS) antigens induced by live attenuated sporozoite vaccines were associated with protection in human challenge experiments. To further evaluate protection mediated by LS antigens, we focused on exported pre-erythrocytic proteins (exported protein 1 (EXP1), profilin (PFN), exported protein 2 (EXP2), inhibitor of cysteine proteases (ICP), transmembrane protein 21 (TMP21), and upregulated in infective sporozoites-3 (UIS3)) expressed in all Plasmodium species and designed optimized, synthetic DNA (synDNA) immunogens. SynDNA antigen cocktails were tested with and without the molecular adjuvant plasmid IL-33. Immunized animals developed robust T cell responses including induction of antigen-specific liver-localized CD8+ T cells, which were enhanced by the co-delivery of plasmid IL-33. In total, 100% of mice in adjuvanted groups and 71%-88% in non-adjuvanted groups were protected from blood-stage disease following Plasmodium yoelii sporozoite challenge. This study supports the potential of synDNA LS antigens as vaccine components for malaria parasite infection.

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Phenotypic Analysis of Rodent Malaria Parasite Asexual and Sexual Blood Stages and Mosquito Stages

Cited by 13 publications

References 0 publications

Highly Sensitive and Rapid Characterization of the Development of Synchronized Blood Stage Malaria Parasites Via Magneto-Optical Hemozoin Quantification

Highly Sensitive and Rapid Characterization of the Development of Synchronized Blood Stage Malaria Parasites Via Magneto-Optical Hemozoin Quantification

An Attenuated HSV-1-Derived Malaria Vaccine Expressing Liver-Stage Exported Proteins Induces Sterilizing Protection against Infectious Sporozoite Challenge

Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from Plasmodium Challenge in a Mouse Model

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