Phenotypic analyses of Salmonella enterica serovar Enteritidis strains isolated in the pre- and post-epidemic period in Brazil

Abstract

Salmonella Enteritidis has caused, since the 1980s, a sustained epidemic of human infections in many countries. This study analyzed S. Enteritidis strains isolated before and after the epidemic period in Brazil regarding their capacities to survive to acid, oxidative, and high-temperature stresses, and capacity to grow in egg albumen. Moreover, the ability to invade human epithelial cells (Caco-2) and to survive inside human (U937) and chicken (HD11) macrophages was checked. Post-epidemic strains showed a better ability to survive after 10 min under acid stress at 37°C (P ≤ 0.05). However, both groups of strains showed similar ability to survive after 1 h under acid stress at 37°C and at 42°C independently of the time of exposure. Similar ability was verified in both groups of strains regarding oxidative stress, growth in egg albumen, high-temperature stress, invasion to Caco-2 cells, and invasion and survival in macrophages. In conclusion, post-epidemic S. Enteritidis strains showed a better ability to survive under the acid stress found in the stomach, which might be an advantage to reach the intestine and colonize chickens and humans. However, both groups of strains did not differ significantly in the majority of the phenotypic tests analyzed in this study.

Application of HPMCAS-coated Ctx(Ile21)-Ha peptide microparticles as a potential use to prevent systemic infection caused bySalmonellaEnteritidis in poultry

“…Salmonella enterica subsp. enterica serovar Enteritidis is one of the leading cause of foodborne diseases posing global concerns to one health and economy [1][2][3] . In this concern, several efforts have been made to reduce the contamination and spread of Salmonella Enteritidis along the poultry production chain, since this serovar is most often associated with poultry products 4,5 .…”

“…In this concern, several efforts have been made to reduce the contamination and spread of Salmonella Enteritidis along the poultry production chain, since this serovar is most often associated with poultry products 4,5 . Besides that, the overuse of antimicrobial agents in animal husbandry, have contributed to the emergence of virulent and multidrug resistant (MDR) strains among poultry products, which represents a critical public health issue, once it could have implications on human health 2,6,7 .…”