Phenotypes of IRS-2 Deficient Mice Produced by Reproductive Technology are Stable

Hashimoto, Haruo; Arai, Toshiro; Ohnishi, Yasuyuki; Eto, Tomoo; Ito, Mamoru; Hioki, Kyoji; Suzuki, Ryo; Yamauchi, Toshimasa; Ohsugi, Mitsuru; Saito, Muneo; Ueyama, Yoshito; Tobe, Kazuyuki; Kadowaki, Takashi; Tamaoki, Norikazu; Kosáka, Kinori

doi:10.1538/expanim.56.149

Cited by 6 publications

(10 citation statements)

References 18 publications

(18 reference statements)

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“…Data for the B6J-Irs2 -/-mouse are presented in this paper. The descriptions and data in the following sections are cited and abstracted from previous publications [2,[7][8][9][10].…”

Section: Irs2mentioning

confidence: 99%

Study on Establishment of Congenic Strains and Screening of Characteristics in IRS-2 Deficient Mice to Support Translational Research on Type 2 Diabetes

Hashimoto

2011

Exp. Anim.

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Abstract:In research into type 2 diabetes, diet-based approaches, i.e., nutritional intake, are important approaches for therapeutic research. We would like to make the following two proposals from the standpoint of laboratory animal science for reproducible animal studies using type 2 diabetes mouse models. These include congenic strains of diabetes mouse models and improvement of diets used in daily care and management. In this research, the Irs2homo-knockout mouse with both impaired glucose tolerance and insulin resistance, and thus type 2 diabetes, was established as a congenic strain. The effect of the genetic background on the onset of diabetes was examined. Next, we discussed which diets are appropriate for general care and management of mouse models in which the pathophysiology is controlled by nutritional conditions. Therefore, we prepared diets by converting the current Japanese and US diets to mice and adjusting the diet contents accordingly. We compared the insulin signals such as those of the liver, pancreas and white fat. We were thus able to establish an evaluation system closer to diabetes in the current population. Using this data as an example, we should consider the quality and ordinary diet of animals as important factors in animal experiments.

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“…Data for the B6J-Irs2 -/-mouse are presented in this paper. The descriptions and data in the following sections are cited and abstracted from previous publications [2,[7][8][9][10].…”

Section: Irs2mentioning

confidence: 99%

Study on Establishment of Congenic Strains and Screening of Characteristics in IRS-2 Deficient Mice to Support Translational Research on Type 2 Diabetes

Hashimoto

2011

Exp. Anim.

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“…The noise levels were measured using a digital noise meter, SM-325 (AS ONE Corporation). All mice were provided with a radiation sterilized (50 kGy) feed for laboratory animals (CL-2, CLEA, Tokyo, Japan) and autoclave-sterilized (124℃, 15 …”

Section: Animalsmentioning

confidence: 99%

“…In research on intestinal flora and probiotics, animal experiments using germfree and gnotobiotic animals reared in vinyl isolators are essential [3]. Because insulin secretion as the pivotal index in diabetes research tends to vary in accordance with circadian rhythm [11,19], insulin secretion and sensitivity are usually evaluated by the glucose tolerance test (GTT) and the insulin tolerance test (ITT) [15,16,41]. However, sample correction for mice reared in vinyl isolators was performed by sacrifice under anesthesia, and in the insulin and glucose evaluations, factors of circadian rhythm cannot be eliminated and it is not possible to determine changes in the same individual mice.…”

Section: Introductionmentioning

confidence: 99%

Vinyl Isolator Breeding Induces Insulin Resistance in C57BL/6JJcl Mice

et al. 2011

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Abstract:As basic probiotics studies, the glucose tolerance test (GTT), insulin tolerance test (ITT), and adipokine and hepatic enzyme activities were investigated in male C57BL/6JJcl (B6J) mice under germfree (GF) or specific pathogen free (SPF) conditions. GF B6J mice were reproduced by reproductive engineering and cesarean section using a vinyl isolators (GF group). Some GF group mice were transferred to other vinyl isolators under SPF conditions (SPF group). In addition, conventional B6J mice bred in an open room were defined as controls (Conv group). GTT, ITT, and the sampling of blood, liver, white adipose tissue, and pancreas were performed when these B6J mice were at the age of 8 weeks. As a result, the GF and SPF groups showed hyperglycemia, impaired glucose tolerance and insulin resistance when compared with the Conv group. The adipose tissues and plasma TNFa concentrations in the GF and SPF groups were enlarged and increased when compared with the Conv group. Hepatic enzyme activities associated with glucose uptake in the GF and SPF groups were higher than those in the Conv group. However, hepatic enzyme activities associated with gluconeogenesis in the GF and SPF groups were lower than those in the Conv group. We assumed that these results were reactions by the liver to recover from the impaired glucose tolerance and the insulin resistance caused by vinyl isolator breeding of the GF and SPF groups by control of glucose metabolism.

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“…1B), because the reproduction indexes of IVF-ET showed that the expansion of Irs2 -/-mice using IVF-ET only was impossible. Hashimoto et al reported that the phenotypes of Irs2 -/-mice were not affected by the reproduction method using IVF-ET [2]. In addition, use of vitrification [8] in IVF-ET might produce better results (Fig.…”

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confidence: 99%

An Efficient Reproductive Method for Irs2-/- Mice with C57BL/6JJcl Genetic Background

et al. 2008

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Efficient reproduction using natural mating and reproduction technology [in vitro fertilization (IVF) and embryo transfer (ET)] was investigated in IRS2 deficientReproductive efficiency in diabetic animals is usually lower than that in the wild type because their sexual behavior [11,12] and embryonic implantation [5,6,9] are suppressed by hyperglycemia. It is known that type 2 diabetes mellitus is associated with disorders of insulin receptor substrates (IRS), which mediate pleiotropic signals initiated by receptors for insulin and adipokines [4]. To obtain a typical type 2 diabetes model, we backcrossed C57BL/6JJcl background for more than 10 generations to establish an inbred line of Irs2 tm1Tka homozy-

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Phenotypes of IRS-2 Deficient Mice Produced by Reproductive Technology are Stable

Abstract: We studied the impact of "IVF -ET" on the glucose tolerance test (GTT), insulin tolerance test (ITT) and adiponectin to investigate differences in the phenotypes of B6J-

Cited by 6 publications

References 18 publications

Study on Establishment of Congenic Strains and Screening of Characteristics in IRS-2 Deficient Mice to Support Translational Research on Type 2 Diabetes

Study on Establishment of Congenic Strains and Screening of Characteristics in IRS-2 Deficient Mice to Support Translational Research on Type 2 Diabetes

Vinyl Isolator Breeding Induces Insulin Resistance in C57BL/6JJcl Mice

An Efficient Reproductive Method for Irs2-/- Mice with C57BL/6JJcl Genetic Background

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