Phenotype plasticity and immunocytochemical evidence for ChAT and DβH co-localization in fetal pig superior cervical ganglion cells

Wang, Jun Ming; Partoens, P.; Callebaut, Dirk P.; Coen, Edmond; Martin, Jean‐Jacques; Potter, W.P. De

doi:10.1016/0165-3806(96)83482-1

Cited by 10 publications

(6 citation statements)

References 24 publications

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“…The co-localization of cholinergic and catecholaminergic markers has already been observed in STG neurons projecting to the pig bulbospongiosus muscle, 72 to the retractor penis muscle in the male pig 73 , and to the sow retractor clitoridis muscle 74 . Moreover, ChAT and DßH were found co-localized in the cranial cervical ganglion of fetal pig 75 . It is known that, during fetal development, some neurons transiently express the noradrenergic phenotype but subsequently lose their noradrenergic characteristics, becoming cholinergic cells 76-78 .…”

Section: Discussionmentioning

confidence: 72%

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Localization of peripheral autonomic neurons innervating the boar urinary bladder trigone and neurochemical features of the sympathetic component

et al. 2013

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The urinary bladder trigone (UBT) is a limited area through which the majority of vessels and nerve fibers penetrate into the urinary bladder and where nerve fibers and intramural neurons are more concentrated. We localized the extramural post-ganglionic autonomic neurons supplying the porcine UBT by means of retrograde tracing (Fast Blue, FB). Moreover, we investigated the phenotype of sympathetic trunk ganglia (STG) and caudal mesenteric ganglia (CMG) neurons positive to FB (FB+) by coupling retrograde tracing and double-labeling immunofluorescence methods. A mean number of 1845.1±259.3 FB+ neurons were localized bilaterally in the L1-S3 STG, which appeared as small pericarya (465.6±82.7 µm2) mainly localized along an edge of the ganglion. A large number (4287.5±1450.6) of small (476.1±103.9 µm2) FB+ neurons were localized mainly along a border of both CMG. The largest number (4793.3±1990.8) of FB+ neurons was observed in the pelvic plexus (PP), where labeled neurons were often clustered within different microganglia and had smaller soma cross-sectional area (374.9±85.4 µm2). STG and CMG FB+ neurons were immunoreactive (IR) for tyrosine hydroxylase (TH) (66±10.1% and 52.7±8.2%, respectively), dopamine beta-hydroxylase (DβH) (62±6.2% and 52±6.2%, respectively), neuropeptide Y (NPY) (59±8.2% and 65.8±7.3%, respectively), calcitonin-gene-related peptide (CGRP) (24.1±3.3% and 22.1±3.3%, respectively), substance P (SP) (21.6±2.4% and 37.7±7.5%, respectively), vasoactive intestinal polypeptide (VIP) (18.9±2.3% and 35.4±4.4%, respectively), neuronal nitric oxide synthase (nNOS) (15.3±2% and 32.9±7.7%, respectively), vesicular acetylcholine transporter (VAChT) (15±2% and 34.7±4.5%, respectively), leuenkephalin (LENK) (14.3±7.1% and 25.9±8.9%, respectively), and somatostatin (SOM) (12.4±3% and 31.8±7.3%, respectively). UBT-projecting neurons were also surrounded by VAChT-, CGRP-, LENK-, and nNOSIR fibers. The possible role of these neurons and fibers in the neural pathways of the UBT is discussed.

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Section: Discussionmentioning

confidence: 72%

“…Immunoreactivity for VIP has already been found, with distinct cranio-caudal differences, in some neurons of the pig cervical, 82 thoracolumbar 48,83 and sacral STG 75 . Quantitative differences in the positivity for this peptide have been found also within the pig UBT by different researchers.…”

Section: Discussionmentioning

confidence: 85%

Localization of peripheral autonomic neurons innervating the boar urinary bladder trigone and neurochemical features of the sympathetic component

et al. 2013

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“…Superior cervical ganglion (SCG) cells. Primary neuronal cultures were prepared from SCG from porcine fetuses collected at the slaughterhouse (gestation stage between 6?5 and 10 weeks) as adapted from Wang et al (1995).…”

Section: Cellsmentioning

confidence: 99%

Higher resistance of porcine trigeminal ganglion neurons towards pseudorabies virus-induced cell death compared with other porcine cell types in vitro

Geenen

Favoreel

Nauwynck

2005

Journal of General Virology

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Trigeminal ganglion (TG) neurons are important target cells for many alphaherpesviruses, constituting major sites for latency/reactivation events. Here, the in vitro kinetics of productive infection of the swine alphaherpesvirus pseudorabies virus (PRV) and resulting cell death in primary porcine TG neurons were determined, and these were compared with similar kinetics in many other porcine cell types. Confocal microscopy showed that all TG neurons expressed late genes such as viral glycoproteins, and that these glycoproteins were processed through the Golgi and reached the cell surface as in other cell types, albeit with a delay of ±2-6 h. However, TG neurons were much more resistant towards PRV-induced cell death compared with all other porcine cell types tested (non-neuronal TG cells, superior cervical ganglion neurons, epithelial kidney cells, arterial endothelial cells, dermal fibroblasts and cells derived from a porcine swine kidney cell line). About half of the TG neurons survived up to 96 h post-inoculation (end of experiment), whereas all other cell types almost completely succumbed within 2 days post-inoculation. In addition, infection with a strongly pro-apoptotic PRV strain that misses the anti-apoptotic US3 protein did not lead to substantial apoptosis in TG neurons, even at 72 h post-inoculation. Thus, primary porcine TG neurons can be infected with PRV in vitro, and are remarkably more resistant to PRV-induced cell death compared with other porcine cell types, suggesting a cell type-specific resistance to alphaherpesvirus-induced cell death that may have important implications for different aspects of the virus life cycle, including latency/reactivation events.

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“…Bovine liver was used as a positive control. Lymphocytes, isolated as previously described (42), were used as a negative control. PCR products were directly sequenced by an automated system using fluorescence-labeled dideoxynucleotides (Applied Biosystems, Foster City, CA).…”

Section: Methodsmentioning

confidence: 99%

Presence of cellular renin-angiotensin system in chromaffin cells of bovine adrenal medulla

Wang

Slembrouck

Tan

et al. 2002

American Journal of Physiology-Heart and Circulatory Physiology

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The presence of a local renin-angiotensin system has been established in organs that serve as angiotensin targets. In this study, the expression of angiotensinogen mRNA and subcellular localization of renin, angiotensin-converting enzyme, and angiotensin II were investigated in bovine adrenal medullary cells in primary culture. By light microscopy, expression of angiotensinogen mRNA, immunoreactive renin, angiotensin-converting enzyme, and angiotensin II were readily detectable only in the chromaffin cells. The density distribution of renin and angiotensin II in sucrose gradients suggested a concentration in chromaffin granules, a localization directly confirmed by immunoelectron microscopy. Reverse transcriptase-polymerase chain reaction and sequencing confirmed the expression of angiotensinogen in bovine chromaffin cells and the adrenal medulla. In addition, in vitro autoradiography indicated that both angiotensin-converting enzyme and angiotensin type 1 receptors were present in the adrenal medulla. These results provide the first direct evidence that chromaffin cells in the adrenal medulla are not only the target for angiotensin but should also be considered as potential local angiotensin-generating and -storing cells.

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Phenotype plasticity and immunocytochemical evidence for ChAT and DβH co-localization in fetal pig superior cervical ganglion cells

Cited by 10 publications

References 24 publications

Localization of peripheral autonomic neurons innervating the boar urinary bladder trigone and neurochemical features of the sympathetic component

Localization of peripheral autonomic neurons innervating the boar urinary bladder trigone and neurochemical features of the sympathetic component

Higher resistance of porcine trigeminal ganglion neurons towards pseudorabies virus-induced cell death compared with other porcine cell types in vitro

Presence of cellular renin-angiotensin system in chromaffin cells of bovine adrenal medulla

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