Phenotype of porcine monocytic cells: modulation of surface molecule expression upon monocyte differentiation into macrophages

McCullough, Kenneth C.; Schaffner, R.; Natale, Valerie; Kim, Yoon B.; Summerfield, Artur

doi:10.1016/s0165-2427(97)00045-7

Cited by 50 publications

(40 citation statements)

References 16 publications

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“…SWC8 is used to discriminate blood and bone marrow-derived granulocytes (SWC8 + ) from monocytes/macrophages (SWC8 – ) [23]. Swine CD14 is expressed at low levels in neutrophils, whereas monocytes/macrophages express higher levels of CD14 [24]. …”

Section: Resultsmentioning

confidence: 99%

An Activated Immune and Inflammatory Response Targets the Pancreas of Newborn Pigs with Cystic Fibrosis

et al. 2011

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Background/Aims: In cystic fibrosis (CF), pancreatic disease begins in utero and progresses over time to complete destruction of the organ. Although inflammatory cells have been detected in the pancreas of humans and pigs with CF, their subtypes have not been characterized. Methods: Using four-color flow cytometry, we analyzed the surface antigens of leukocytes in pancreas, blood, and mesenteric lymph nodes (MLN) of newborn pigs with CF (CFTR^–/– and CFTR^Δ^F508/^Δ^F508) and in those without CF (CFTR^+/–, CFTR^+/^Δ^F508, CFTR^+/+). Pancreatic histopathology was examined with HE stain. Results: CF pig pancreas had patchy distribution of inflammatory cells with neutrophils/macrophages in dilated acini, and lymphocytes in the interstitium compared to non-CF. B cells, effector (MHC-II⁺) and cytotoxic (CD2⁺CD8⁺) γδ T cells, activated (MHC-II⁺ and/or CD25⁺) and effector (CD4⁺CD8⁺) αβ T helper cells, effector natural killer cells (MHC-II⁺CD3^–CD8⁺), and monocytes/macrophages and neutrophils were increased in the CF pig pancreas compared to pigs without CF. Blood and MLN leukocyte populations were not different between CF and non-CF pigs. Conclusions: We discovered an activated immune response that was specific to the pancreas of newborn CF pigs; inflammation was not systemic. The presence of both innate and adaptive immune cells suggests that the disease process is complex and extensive.

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Section: Resultsmentioning

confidence: 99%

An Activated Immune and Inflammatory Response Targets the Pancreas of Newborn Pigs with Cystic Fibrosis

et al. 2011

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“…Compared to monocytes, most DC expressed higher levels of SLA I and SLA II antigens (DQ and DR), and co-stimulatory molecules CD80/CD86. A large number of these cells expressed SWC9, a marker that has been previously associated with mature macrophages (Dominguez et al, 1998;McCullough et al, 1997). CD1a expression also increased during the differentiation of monocytes into DC, from 2876% up to 62711% on day 3, declining thereafter.…”

Section: Characterisation Of DC Derived From Swine Monocytesmentioning

confidence: 86%

In vitro differentiation of porcine blood CD163− and CD163+ monocytes into functional dendritic cells

et al. 2004

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“…The intensity of CD14 labelling depends on the cell type: PMN (polymorph nuclear leukocytes) neutrophils express low levels of CD14, whereas monocytes/macrophages (macrophages less than monocytes [128]) express higher levels of CD14. CD14 can also be found on monocyteand bone marrow-derived GM-CSF-driven DC, although functional differences are found when compared to monocyte CD14.…”

Section: Cd14mentioning

confidence: 99%

Membrane markers of the immune cells in swine: an update

Piriou-Guzylack¹,

2008

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-Besides their breeding value, swine are increasingly used as biomedical models. As reported in three international swine clusters of differentiation (CD) workshops and in the animal homologue section of the last workshop for the determination of human leukocyte differentiation antigens (HLDA 8), characterisation of leukocyte surface antigens by monoclonal antibodies and other molecular studies have determined the cell lineages and blood leukocyte subsets implicated in the immune response, including cell adhesion molecules involved in cell trafficking. This review focusses on the current state of knowledge of porcine leukocyte differentiation and major histocompatibility complex (SLA) molecules. Examples of porcine particularities such as the double-positive T lymphocytes with the phenotype CD4 + CD8 low and CD4 − CD8 low T cell subsets and the persistence of SLA class II after T-lymphocyte activation are illustrated, as well as the shared characteristics of the Artiodactyla group, such as the high proportion of TcR (T cell receptor) T cells in blood and other lymphoid tissues. Furthermore, discrepancies between swine and humans, such as CD16 expression on dendritic cells and CD11b (wCD11R1) tissue distribution are outlined. The rapidly growing information should facilitate manipulation of the swine immune system towards improving disease control, and open new avenues for biomedical research using the pig as a model. cluster of differentiation (CD) / monoclonal antibody / immune system / histocompatibility system / pig

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Phenotype of porcine monocytic cells: modulation of surface molecule expression upon monocyte differentiation into macrophages

Cited by 50 publications

References 16 publications

An Activated Immune and Inflammatory Response Targets the Pancreas of Newborn Pigs with Cystic Fibrosis

An Activated Immune and Inflammatory Response Targets the Pancreas of Newborn Pigs with Cystic Fibrosis

In vitro differentiation of porcine blood CD163− and CD163+ monocytes into functional dendritic cells

Membrane markers of the immune cells in swine: an update

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