Phenotype of NK Cells Determined on the Basis of Selected Immunological Parameters in Children Treated due to Acute Lymphoblastic Leukemia

Kołtan, Sylwia; Dębski, Robert; Kołtan, Andrzej; Grześk, Elżbieta; Tejza, Barbara; Eljaszewicz, Andrzej; Gackowska, Lidia; Kubicka, Małgorzata; Kołodziej, Beata; Kuryło-Rafińska, Beata; Kubiszewska, Izabela; Wiese, Małgorzata; Januszewska, Milena; Michałkiewicz, Jacek; Wysocki, Mariusz; Styczyński, Jan; Grześk, Grzegorz

doi:10.1097/md.0000000000002369

Cited by 6 publications

(17 citation statements)

References 56 publications

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“…The anti‐leukemia cytotoxicity of NK cells has been demonstrated in vitro in coculture assays, and by analysis of the effects of NK cell grafts on leukemia development in stem cell transplantation model . The correlation between the NK activity and leukemia progression in patients also supports the concept that leukemia development can be inhibited by NK cells .…”

Section: Introductionmentioning

confidence: 83%

NK cell‐mediated anti‐leukemia cytotoxicity is enhanced using a NKG2D ligand MICA and anti‐CD20 scfv chimeric protein

et al. 2018

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NK cells are important innate cytotoxic lymphocytes that have potential in treatment of leukemia. Engagement of NKG2D receptor on NK cells enhances the target cytotoxicity. Here, we produced a fusion protein consisting of the extracellular domain of the NKG2D ligand MICA and the anti-CD20 single-chain variable fragment (scfv). This recombinant protein is capable of binding both NK cells and CD20 tumor cells. Using a human NKG2D reporter cell system we developed, we showed that this fusion protein could decorate CD20 tumor cells with MICA extracellular domain and activate NK through NKG2D. We further demonstrated that this protein could specifically induce the ability of a NK cell line (NKL) and primary NK cells to lyse CD20 leukemia cells. Moreover, we found that downregulation of surface HLA class I expression in the target cells improved NKL-mediated killing. Our results demonstrated that this recombinant protein specifically lyses leukemia cells by NK cells, which may lead to development of a novel strategy for treating leukemia and other tumors.

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Section: Introductionmentioning

confidence: 83%

NK cell‐mediated anti‐leukemia cytotoxicity is enhanced using a NKG2D ligand MICA and anti‐CD20 scfv chimeric protein

et al. 2018

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“…*CD158a, CD158b are markers showing recovery and strengthening of NK-population that produces perforin and granzymes [23].…”

Section: Variety Of Nk-populations Typed By Receptor Lectinsmentioning

confidence: 99%

“…Cytokines and NK-populations Anti-tumor effects produced by inborn immunity become apparent via modulating production of cytokines sets and, as a result, factors that are cytotoxic in relation to tumor cells are produced (or delivered) there where a tumor is localized in a body. Anti-tumor NK-production of interferon (IFN-gamma) [6,15,17,20,22,23,24,29] and tumor necrosis factor (TNF) is induced [15, 24,29]. NK-cells are controlled with interleukins (IL-2, 5, 12, 13, 15, 18) [15, 24,30] and with participation of colony stimulating factor (CSF1) [37].…”

Section: Variety Of Nk-populations Typed By Receptor Lectinsmentioning

confidence: 99%

“…Cytotoxic factors of NK-populations As phenotypes are transformed into relevant cytotoxic cells populations, granzymes A, B, and K, perforin, and other anti-tumor agents are released in the process [6,19,23]. These factors are in systemic contrast with scattered tumor factors (lactate dehydrogenase, TGF-beta, CSF1, some others) [8, 37,40].…”

Section: Variety Of Nk-populations Typed By Receptor Lectinsmentioning

confidence: 99%

“…*CD56brightNKG2A+-population applied in adjusting EBV-associated lymphomas [22]. *Treatment provided for patients with acute lymphoblastic leukemia via recovery and growth of NK-populations with expression of CD158a, CD158b, perforin, and granzyme K among NK-populations with different granzymes types expression [23].…”

Section: Variety Of Nk-populations Typed By Receptor Lectinsmentioning

confidence: 99%

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NK-cells that identify glycopatterns and their anti-tumor potential against a background of epidemically significant viral infections

Lakhtin¹,

Lakhtin²,

Алешкин³

et al. 2019

Health Risk Analysis

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Risks related to tumors development against a background of viral infections as well as factors that determine such risks or reduce them have not been examined profoundly so far. Our research goal was to accomplish a scientific review of research on a potential possessed by populations of lectin NK-cells (natural killers) in a body; such populations can have variable sets of lectin and other functionally significant cell surface receptors against tumors in a situation when viruses, including epidemically significant ones, penetrate a body. It is shown that cofunctioning of various receptors and their ligands that redistribute cytokines (glycopattern-identifying lectin (basis) receptors, Ig-similar receptors, cytotoxic receptors, and other effector (adjusting) receptors) plays a significant role in intercellular communications and effects produced by NK-populations. NK-populations network is a promising resource for body protection and it should be taken into account when developing new anti-tumor and anti-viralpreventive and medical strategies. When certain NK-populations with protective functions are absent in a body, it can be considered a new multi-factor risk of viral and oncologic diseases in an individual or a contingent living in a specific region. The reviewed data can be applied to develop new anti-tumor and anti-viral medications and vaccines as well as medical strategies. Probiotic lectins are promising ligands of intercellular communications associated with immune surveillance. Read online __________________________  Lakhtin M.V.

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NK Cells as Possible Prognostic Factor in Childhood Acute Lymphoblastic Leukemia

Mizia‐Malarz

Sobol‐Milejska

2019

Disease Markers

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Deficiency or impaired function natural killer (NK) cells might result in the development of serious infections and promote the development of malignancies. The aim of our study was to assess the prognostic role of NK cell percentage in bone marrow on the day of acute lymphoblastic leukemia (ALL) diagnosis. 84 children (49 males = 58%; median age 5 yrs) with ALL were enrolled. The NK cell percentage was assessed using flow cytometry with antibodies against the cluster of differentiation (CD): CD3, CD56, and CD16. We evaluated two groups: group I (NK+), patients with NK cells in the bone marrow (n = 74), and group II (NK-), patients without NK cells in the bone marrow (n = 10) (cut-off value of negative <1%). In the patients from group I, the prednisone good response on day 8 and the remission on day 15 of treatment were observed significantly more often (p = .01, p = .03). The children from group I had significantly better survival as compared to those from group II (p = .02) (HR 2.59; 95% CI: 1.38-4.85). The presence of NK cells in the bone marrow at diagnosis can be a prognostic factor in children with ALL. The presented results should be the basis for further research.

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Phenotype of NK Cells Determined on the Basis of Selected Immunological Parameters in Children Treated due to Acute Lymphoblastic Leukemia

Cited by 6 publications

References 56 publications

NK cell‐mediated anti‐leukemia cytotoxicity is enhanced using a NKG2D ligand MICA and anti‐CD20 scfv chimeric protein

NK cell‐mediated anti‐leukemia cytotoxicity is enhanced using a NKG2D ligand MICA and anti‐CD20 scfv chimeric protein

NK-cells that identify glycopatterns and their anti-tumor potential against a background of epidemically significant viral infections

NK Cells as Possible Prognostic Factor in Childhood Acute Lymphoblastic Leukemia

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