Phenotype-Directed DNA Nanomachine for in Situ Analysis of Stem Cell-like Subpopulation in Breast Cancer

Force-Encoding DNA Nanomachines for Simultaneous and Direct Detection of Multiple Pathogenic Bacteria in Blood

“…The advancement of DNA nanotechnology has paved the way for the design and integration of various DNA nanomaterials into biosensors, resulting in the development of innovative biosensors with enhanced performance. − The unique characteristics of DNA nanomaterials, such as their small size and surface effects, perfectly align with the demands for multifunctionality and miniaturization in biosensors . Aptamer-based DNA nanodevices have emerged as crucial components in the domain of biological detection, owing to their exceptional capacity to specifically recognize target molecules. , Aptamers directly engage with the outer surface of bacteria instead of targeting specific nucleic acids, leading to streamlined detection of intact bacteria without the need for laborious sample preparations. ,− In addition, DNA can be utilized to construct nanoscale molecular machines (e.g., walkers, tweezers, and amplifiers) that can respond to targets on membrane surfaces or even inside vesicles, demonstrating its charm in the field of disease diagnosis. − In these ingenious molecular machines, DNA walkers have found extensive applicability in signal amplification strategies, employing toehold-mediated strand displacement or enzyme-mediated DNA hydrolysis. − By means of self-assembly or integration with other nanomaterials, DNA nanodevices hold tremendous promise in addressing the challenges encountered in clinical applications.…”