Phenotype and RNA-seq-Based transcriptome profiling of Staphylococcus aureus biofilms in response to tea tree oil

Zhao, Xingchen; Liu, Zonghui; Liu, Zuojia; Meng, Rizeng; Shi, Ce; Chen, Xiangrong; Bu, Xiujuan; Guo, Na

doi:10.1016/j.micpath.2018.07.027

Cited by 38 publications

(26 citation statements)

References 56 publications

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“…SarA is a cytoplasmic transcriptional regulatory protein that activates the agr operon and independent of the QS system, controls the expression of many virulence genes in SA cells that are involved in biofilm formation, such as some matrix adhesion genes (e.g., fnbA, encoding fibronectin-binding protein A). In the present work, sarA was downregulated [69]. In a previous study, EO distilled from Chamaecyparis obtusa leaves (with 19 % sabinene) reduced the expression of several regulator genes such as argA (at 0.2 mg/mL) and sarA (at 0.3 mg/mL), which resulted in a decreased production of virulence factors in SA [70].…”

Section: Inhibition Of Quorum Sensing-regulated Formation Of Biofilmssupporting

confidence: 59%

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Anti-biofilm and Virulence Factor-Reducing Activities of Essential Oils and Oil Components as a Possible Option for Bacterial Infection Control

Reichling

2020

Planta Med

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Pathogenic biofilm-associated bacteria that adhere to biological or nonbiological surfaces are a big challenge to the healthcare and food industries. Antibiotics or disinfectants often fail in an attempt to eliminate biofilms from those surfaces. Based on selected experimental research, this review deals with the potential biofilm-inhibiting, virulence factor-reducing, and biofilm-eradicating activities of essential oils and single essential oil compounds using Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Chromobacterium violaceum as model organisms. In addition, for the bacteria reviewed in this overview, different essential oils and essential oil compounds were reported to be able to modulate the expression of genes that are involved in the formation of autoinducer molecules, biofilms, and virulence factors. The anti-quorum sensing activity of some essential oils and single essential oil compounds was demonstrated using the gram-negative bacterium C. violaceum. Reporter strains of this bacterium produce the violet-colored compound violacein whose synthesis is regulated by quorum sensing autoinducer molecules called acylhomeserinlactones. Of great interest was the discovery that enantiomeric monoterpenes affected the quorum sensing regulation system in different ways. While the (+)-enantiomers of carvone, limonene, and borneol increased violacein formation, their (−)-analogues inhibited violacein production.For the successful eradication of biofilms and the bacteria living inside them, it is absolutely necessary that the lipophilic volatile substances can penetrate into the aqueous channels of biofilms. As shown in recent work, hydrophilic nano-delivery systems encapsulating essential oils/essential oil compounds with antibacterial effects may contribute to overcome this problem.

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Section: Inhibition Of Quorum Sensing-regulated Formation Of Biofilmssupporting

confidence: 59%

“…In a recent experimental study, TTO was investigated for its anti-biofilm activity in SA ATCC 29213 [69]. The most interesting focus of the work was the question of whether TTO influences the gene expression profile (transcriptome analysis) of biofilm-borne bacterial cells.…”

Section: Inhibition Of Quorum Sensing-regulated Formation Of Biofilmsmentioning

confidence: 99%

Anti-biofilm and Virulence Factor-Reducing Activities of Essential Oils and Oil Components as a Possible Option for Bacterial Infection Control

Reichling

2020

Planta Med

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“…In the biofilm formation of Bacillus amylobacillus , the antimicrobial peptide gene lci and the drug resistance gene yvqHI were among the most up-regulated genes in induction of systemic resistance ( Kröber et al, 2016 ). RNA-seq results showed that added tea tree oil caused differential expression of 304 genes in Staphylococcus aureus biofilms compared to planktonic cells ( Zhao et al, 2018 ). Although biofilms made by pathogens have been studied extensively, analyses of L. plantarum biofilm formation are still scarce.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic analysis of Lactiplantibacillus plantarum RS66CD biofilm in high-salt conditions and planktonic cells

Yan

Liu

et al. 2020

PeerJ

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Background Lactiplantibacillus plantarum (L. plantarum), a dominant strain in traditional fermented foods, is widely used in fermentation industry because of its fast acid production. However, L. plantarum is easily inactivated due to acidity, high temperature and other factors. The formation of biofilm by bacteria can effectively increase environmental tolerance. Therefore, it is important to improve the environmental tolerance of L. plantarum by studying its biofilm formation conditions and regulatory mechanisms. Methods After determining a suitable NaCl concentration for promoting biofilm formation, L. plantarum was grown with 48 g L−1 NaCl. Differential gene expressions in L. plantarum biofilm vs. planktonic cells were analyzed using RNA sequencing and validated using qPCR. Result L. plantarum RS66CD biofilm formation formed highest amount of when grown at 48 g L−1 NaCl. Altogether 447 genes were up-regulated and 426 genes were down-regulated in the biofilm. KEGG pathway analysis showed that genes coding for D-Alanine metabolism, peptidoglycan biosynthesis, two-component system, carbon metabolism, bacterial secretion system, lysine biosynthesis and fatty acid metabolism were crucial for biofilm formation. In addition, eight other genes related to biofilm formation were differentially expressed. Our results provide insights into the differential gene expression involved in biofilm formation, which can help to reveal gene regulation during L. plantarum biofilm formation.

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“…Considering the importance of a better understanding of the bacterial behavior in carious sites 2 , the current research established a methodology for RNA extraction and isolation to enable bacterial gene expression studies from human carious dentine samples. The majority of transcriptional studies of cariogenic bacteria was performed in planktonic cells in vitro and in vivo biofilms 7,9,11 , substrates where higher bacterial concentration is found when compared to dentine caries lesions 19 .…”

Section: Discussionmentioning

confidence: 99%

“…Among them, RNA-based analysis focus in detection of metabolically active microbial members and identification of 145 genes expressed under different circumstances 2,3 . The majority of RNA-based investigations of oral microbiota analyzed only planktonic cells 4 , in vitro biofilms 5,6,7 or in vivo biofilms from rodent animals 8,9 . Although the first transcriptomic studies of human dental biofilm 10,11 and dentine 12,13 were carried out, just a few articles showed is given about technical procedures, RNA yield, purity and integrity from these human samples studies 13,14 .…”

Section: Introductionmentioning

confidence: 99%

Extraction and purification of RNA from human carious dentine: an approach to enable bacterial gene expression studies

et al. 2019

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Background: RNA isolation from bacteria within dentine caries lesions could be difficult due to reduced amount of collectable biomass and high mRNA instability. Attempting to overcome this challenge we describe one protocol developed to extract and purify total RNA from dentine lesions. Objective: customize a bacterial RNA extraction and purification method from human carious dentine. Methods: quantity and purity of extracted RNA were measured with a microvolume UV-VIS spectrophotometer, RNA integrity was assessed by standard denaturing agarose gel electrophoresis and images were captured under ultraviolet light with camera and analyzed. DNase treatment removed genomic DNA and an additional step of purification was carried out in silica spin column. Results: final yield (ng/μl) was 67.01 ± 22.33, absorbance ratio A260/A280 = 2.0 ± 0.07 and RNA integrity were obtained. The purified samples were reversely transcribed and the expression of atpD and fabM gene from Streptococcus mutans analyzed by quantitative real-time PCR. Conclusion: the extraction methodology developed produced high-quality RNA from dentine microbiota for transcriptional analysis.

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Phenotype and RNA-seq-Based transcriptome profiling of Staphylococcus aureus biofilms in response to tea tree oil

Cited by 38 publications

References 56 publications

Anti-biofilm and Virulence Factor-Reducing Activities of Essential Oils and Oil Components as a Possible Option for Bacterial Infection Control

Anti-biofilm and Virulence Factor-Reducing Activities of Essential Oils and Oil Components as a Possible Option for Bacterial Infection Control

Comparative transcriptomic analysis of Lactiplantibacillus plantarum RS66CD biofilm in high-salt conditions and planktonic cells

Extraction and purification of RNA from human carious dentine: an approach to enable bacterial gene expression studies

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