Diabetes is an important public health problem, and it is well known that healing processes are impaired in diabetic wounds, one of its complications. Keratins are structural proteins found in skin cells and play a vital role in wound healing and skin integrity. While there is increasing interest in the anti-inflammatory properties of metformin, a drug commonly used for diabetes, its potential effect on wound healing and keratins is not yet fully understood. In this context, we aimed to evaluate how metformin administration affects keratin 6 and keratin 16 expression at both mRNA and protein levels. In our study on diabetic rats, we investigated the effects of topically administered metformin on keratins in wound healing. We then compared the protein and mRNA expression levels of keratin 6 and keratin 16 in treated wounds with untreated wounds using reverse transcription polymerase chain reaction and immunohistochemistry methods. The results of the study likely detail changes in the expression levels of keratin 6 and keratin 16 after metformin administration. This information will shed light on how metformin affects the molecular mechanisms involved in wound healing, particularly with respect to these important structural proteins. Understanding these changes may provide insight into potential therapeutic approaches to improve diabetic wound healing. By elucidating the effect of metformin on keratin expression, the study may contribute to the development of targeted therapies aimed at improving the healing process in diabetic wounds.