Phenotype and genomic background of Arcobacter butzleri strains and taxogenomic assessment of the species

Fanelli, Francesca; Chieffi, Daniele; Pinto, Angela Di; Mottola, Anna; Baruzzi, Federico; Fusco, Vincenzina

doi:10.1016/j.fm.2020.103416

Cited by 21 publications

(66 citation statements)

References 127 publications

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“…The AMR database created here (ARCO_IBIZ_AMR) contains both specific AMR genes and heavy metal resistance genes, as described before for A. butzleri [ 18 , 26 , 27 ]. In addition, genes were added which, due to their Prokka annotation, were presumed to belong to one of these groups (see Materials and Methods).…”

Section: Resultsmentioning

confidence: 99%

“…coli [ 64 ]. EP9 confers resistance to fosmidomycin because it contains the fsr gene [ 27 ]. Of the 19 EP systems described before by Isidro et al, only four (including the Type I secretion system) were not detected [ 26 ].…”

Section: Resultsmentioning

confidence: 99%

“…For the construction of the databases, previously described AMR, virulence-associated and heavy metal resistance genes were used [ 18 , 26 , 27 ]. Additionally, more resistance genes were added after screening the Prokka annotated assemblies of the genomes RM4018p, Ab_2211p, Ab_4511p, BMH_AB_233Bp, BMH_AB_246Bp, D4963p and L353p provided by Isidro et al [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies mostly examined the antibiotic susceptibility of A. butzleri phenotypically, but in the last few years the underlying antimicrobial resistance (AMR) genes have also been studied more closely [ 18 , 26 , 27 , 28 , 29 , 30 ]. Recently, more virulence-associated genes have been identified in A. butzleri in addition to the previously known homologous genes found in Campylobacter jejuni ( pldA , mviN , irgA , iroE , ciaB , hecA , hecB , cj1345 , cadF , tlyA ) [ 18 , 26 , 27 , 31 ]. The resistance of A. butzleri to heavy metals has hardly been investigated to date [ 18 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, more virulence-associated genes have been identified in A. butzleri in addition to the previously known homologous genes found in Campylobacter jejuni ( pldA , mviN , irgA , iroE , ciaB , hecA , hecB , cj1345 , cadF , tlyA ) [ 18 , 26 , 27 , 31 ]. The resistance of A. butzleri to heavy metals has hardly been investigated to date [ 18 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

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Aliarcobacter butzleri from Water Poultry: Insights into Antimicrobial Resistance, Virulence and Heavy Metal Resistance

Müller

Abdel-Glil

Hotzel

et al. 2020

Genes

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Aliarcobacter butzleri is the most prevalent Aliarcobacter species and has been isolated from a wide variety of sources. This species is an emerging foodborne and zoonotic pathogen because the bacteria can be transmitted by contaminated food or water and can cause acute enteritis in humans. Currently, there is no database to identify antimicrobial/heavy metal resistance and virulence-associated genes specific for A. butzleri. The aim of this study was to investigate the antimicrobial susceptibility and resistance profile of two A. butzleri isolates from Muscovy ducks (Cairina moschata) reared on a water poultry farm in Thuringia, Germany, and to create a database to fill this capability gap. The taxonomic classification revealed that the isolates belong to the Aliarcobacter gen. nov. as A. butzleri comb. nov. The antibiotic susceptibility was determined using the gradient strip method. While one of the isolates was resistant to five antibiotics, the other isolate was resistant to only two antibiotics. The presence of antimicrobial/heavy metal resistance genes and virulence determinants was determined using two custom-made databases. The custom-made databases identified a large repertoire of potential resistance and virulence-associated genes. This study provides the first resistance and virulence determinants database for A. butzleri.

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Section: Resultsmentioning

confidence: 99%