Phenotype and Function of Monocyte-Derived Dendritic Cells from Chinese Rhesus Macaques

Xia, Houjun; Liu, Hongliang; Zhang, Gao-Hong; Zheng, Yong‐Tang

doi:10.1038/cmi.2009.22

Cited by 10 publications

(7 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is now a large body of data relating to the description of in vivo DC and in vitro-propagated macaque DC [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27]. Other OWP species studied include Chinese rhesus macaque [28], African green monkeys [29], and baboon [30,31]. Owl monkeys [32] and the common marmoset [33,34] are the only NWPs, in which DC have been studied.…”

Section: Nhp Models Of DC Biologymentioning

confidence: 99%

“…In African green monkeys, DC-SIGN is strongly expressed in LNs and on the cell surface of MoDC in conjunction with CD11c [29,61]. DC-SIGN is also strongly expressed on MoDC of Chinese macaques [28]. DC-SIGN ϩ cells have successfully been targeted in vivo with anti-human antibodies in cynomolgus monkeys [62].…”

Section: Surface Marker Phenotype Of In Vitro-propagated Nhp DCmentioning

confidence: 99%

See 1 more Smart Citation

Non-human primate dendritic cells

Jesudason

Collins

Rogers

et al. 2011

Journal of Leukocyte Biology

View full text Add to dashboard Cite

Non-human primates (NHP) are essential translational models for biomedical research. Dendritic cells (DC) are a group of antigen presenting cells (APC) that play pivotal roles in the immunobiology of health and disease and are attractive cells for adoptive immunotherapy to stimulate and suppress immunity. DC have been studied extensively in humans and mice but until recently, have not been well characterized in NHP. This review considers the available data about DC across a range of NHP species and summarizes the understanding of in vitro-propagated DC and in vivo-isolated DC, which is now established. It is clear that although NHP DC exist within the paradigm of human DC, there are important functional and phenotypic differences when compared with human DC subsets. These differences need to be taken into account when designing preclinical, translational studies of DC therapy using NHP models.

show abstract

Section: Nhp Models Of DC Biologymentioning

confidence: 99%

Section: Surface Marker Phenotype Of In Vitro-propagated Nhp DCmentioning

confidence: 99%

Non-human primate dendritic cells

Jesudason

Collins

Rogers

et al. 2011

Journal of Leukocyte Biology

View full text Add to dashboard Cite

show abstract

“…For this, we assessed macDC-SIGN expression and clearly observed positive cells, which were lacking in the control animals (Figure 2A). Mac-DCSIGN is the macaque homolog of DC-SIGN in humans and has been characteristic of DC-specific studies in SIV infection (Changyong et al , 2010; de Witte et al , 2008; Xia et al , 2009; Yu Kimata et al , 2002). In general, DC-SIGN is considered a specific marker primarily for the tissue specific DCs (Soilleux et al , 2002), a subset of BDCA-2 + plasmacytoid DC precursors, and monocyte-derived DCs (MDDCs).…”

Section: Discussionmentioning

confidence: 99%

Effect of morphine and SIV on dendritic cell trafficking into the central nervous system of rhesus macaques

et al. 2013

View full text Add to dashboard Cite

Recruitment of immune cells such as monocytes/macrophages and dendritic cells (DCs) across the blood-brain barrier (BBB) has been documented in diseases involving neuroinflammation. Neuroinvasion by HIV leads to neurocognitive diseases and alters the permeability of the BBB. Likewise, many HIV patients use drugs of abuse such as morphine, which can further compromise the BBB. While the role of monocytes and macrophages in neuroAIDS is well established, research demonstrating the presence and role of DCs in the CNS during HIV infection has not been developed yet. In this respect, this study explored the presence of DCs in the brain parenchyma of rhesus macaques infected with a neurovirulent form of SIV (SIV mac239 R71/17E) and administered with morphine. Cells positive for DC markers including CD11c (integrin), macDC-SIGN (dendritic cell-specific ICAM-3 grabbing nonintegrin), CD83 (a maturation factor) and HLA-DR (MHC class II) were consistently found in the brain parenchyma of SIV-infected macaques as well as infected macaques on morphine. Control animals did not exhibit any DC presence in their brains. These results provide first evidence of DCs’ relevance in NeuroAIDS vis-à-vis drugs of abuse and open new avenues of understanding and investigative HIV-CNS inflictions.

show abstract

“…Mature DCs with strong stimulatory properties express CD25 on their cell surface for binding T cell, which is the early mechanism before T cells start to express CD25 [ 27 , 28 ]. The mature DCs exhibit high expressions of activation marker molecules such as CD25 as well as co-stimulatory molecules such as CD40, CD80 and CD86 [ 29 ]. We here observed that those FTH-DCs migrated into popliteal LNs exhibited T cell activation molecule CD25.…”

Section: Discussionmentioning

confidence: 99%

In vivo Tracking of Dendritic Cell using MRI Reporter Gene, Ferritin

et al. 2015

View full text Add to dashboard Cite

The noninvasive imaging of dendritic cells (DCs) migrated into lymph nodes (LNs) can provide helpful information on designing DCs-based immunotherapeutic strategies. This study is to investigate the influence of transduction of human ferritin heavy chain (FTH) and green fluorescence protein (GFP) genes on inherent properties of DCs, and the feasibility of FTH as a magnetic resonance imaging (MRI) reporter gene to track DCs migration into LNs. FTH-DCs were established by the introduction of FTH and GFP genes into the DC cell line (DC2.4) using lentivirus. The changes in the rate of MRI signal decay (R2*) resulting from FTH transduction were analyzed in cell phantoms as well as popliteal LN of mice after subcutaneous injection of those cells into hind limb foot pad by using a multiple gradient echo sequence on a 9.4 T MR scanner. The transduction of FTH and GFP did not influence the proliferation and migration abilities of DCs. The expression of co-stimulatory molecules (CD40, CD80 and CD86) in FTH-DCs was similar to that of DCs. FTH-DCs exhibited increased iron storage capacity, and displayed a significantly higher transverse relaxation rate (R2*) as compared to DCs in phantom. LNs with FTH-DCs exhibited negative contrast, leading to a high R2* in both in vivo and ex vivo T2*-weighted images compared to DCs. On histological analysis FTH-DCs migrated to the subcapsular sinus and the T cell zone of LN, where they highly expressed CD25 to bind and stimulate T cells. Our study addresses the feasibility of FTH as an MRI reporter gene to track DCs migration into LNs without alteration of their inherent properties. This study suggests that FTH-based MRI could be a useful technique to longitudinally monitor DCs and evaluate the therapeutic efficacy of DC-based vaccines.

show abstract

Phenotype and Function of Monocyte-Derived Dendritic Cells from Chinese Rhesus Macaques

Cited by 10 publications

References 20 publications

Non-human primate dendritic cells

Non-human primate dendritic cells

Effect of morphine and SIV on dendritic cell trafficking into the central nervous system of rhesus macaques

In vivo Tracking of Dendritic Cell using MRI Reporter Gene, Ferritin

Contact Info

Product

Resources

About