Phenomenon of Multiple Mutations in the 35S rRNA Genes of the C Subgenome of Polyploid Avena L.

Rodionov, A. V.; Amosova, Аlexandra V.; Krainova, L. M.; Мачс, Э. М.; Mikhaǐlova, Yu. V.; Gnutikov, A. A.; Муравенко, О. В.; Лоскутов, И. Г.

doi:10.1134/s1022795420060095

Cited by 8 publications

(10 citation statements)

References 57 publications

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“…Generally, the phenomenon of nucleolar dominance is observed in hybrids and allopolyploids 47 , 60 . Here, the rDNA sequences of the repressed genome rapidly accumulate SNPs and deletions and are gradually lost 34 , 36 , 38 , 40 , 61 . However, a great many hybrids and species of hybrid origin show greater or lesser intragenomic heterogeneity of ITS 21 , 22 , 31 , 32 .…”

Section: Discussionmentioning

confidence: 99%

“…The first is the retention of part of the rDNA ancestral species in hybrids [29][30][31][32] . The second reason could be pseudogenization of transcriptionally inert rDNA loci with their slow homogenization; it has been shown that in transcriptionally inert rDNA copies the accumulation rate of SNPs and deletions is 10 times higher than in rDNA of transcribed loci 38,40,61,62 . Homogenization of sequences within a single NOR proceeds faster than in different NORs, where it also occurs at varying rates 63,64 .…”

Section: Discussionmentioning

confidence: 99%

“…First-generation interspecific hybrids always have both parents' rDNA in the genome [27][28][29][30] . In the next generations, some species retain rDNA [31][32][33] , while others undergo rDNA homogenization, resulting in the loss of most of the rDNA of one of the parents and only a minor number of 35S rDNA loci remaining in the genome, which can be detected via sequencing cloned PCR products 22,28,[33][34][35] or by using next-generation-sequencing (NGS) 16,21,[36][37][38][39][40][41] . Analysis of the intraindividual polymorphism of 35S rDNA fragments has been successfully used to study hybrids in various groups of plants [29][30][31][32][33] .…”

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confidence: 99%

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Hybridization and diversity of aquatic macrophyte Sparganium L. (Typhaceae) as revealed by high-throughput nrDNA sequencing

Belyakov,

Mikhaylova,

Machs

et al. 2022

Sci Rep

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Sparganium is an emergent aquatic macrophyte widely spread in temperate and subtropical zones. Taxa of this genus feature high phenotypic plasticity and can produce interspecific hybrids. By means of high-throughput sequencing of the internal transcribed spacer (ITS1) of 35S rDNA, the status of 15 Eurasian Sparganium species and subspecies was clarified and the role of hybridization events in the recent evolution of the genus was investigated. It has been shown that a number of species such as S. angustifolium, S. fallax and S. subglobosum have homogenized rDNA represented by one major ribotype. The rDNA of other taxa is represented by two or more major ribotypes. Species with high rDNA heterogeneity are apparently of hybrid origin. Based on the differences in rDNA patterns, intraspecific diversity was identified in S. probatovae and S. emersum. Thus, we have concluded that Sparganium has extensive interspecific hybridization at the subgenus level, and there may also be occasional hybridization between species from different subgenera.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Hybridization and diversity of aquatic macrophyte Sparganium L. (Typhaceae) as revealed by high-throughput nrDNA sequencing

Belyakov,

Mikhaylova,

Machs

et al. 2022

Sci Rep

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“…Using sequencetagged DNA sequencing on the Ro che 454 platform, intragenomic polymorphism of one of the 35S rRNA regions (18S rDNA fragment-ITS1-frag ment of 5.8S rDNA) in three hexaploid Avena species with karyotypes AACCDD and the tetraploid species A. insularis (AAСС or CCDD) has been studied (Rodionov et al, 2020). Instead of the expected 50 % of Cvariant ITS1 in A. insularis and 33 % of Cvariant ITS1 in hexaploids A. fatua, A. ludoviciana, and A. sterilis, the actual rate of C subgenome specific ITSs comprised around 3.3 % of rDNA in A. insularis and 1.4-2.4 % of rDNA in hexaploid genomes.…”

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confidence: 99%

“…In the "repressed" 35S rRNA loci of C subgenomes, multiple mutations were apparently not accompanied by homogeni zation of rDNA. Hence, there is a reason to believe that the processes of rDNA isogenization and the process of transcription/silencing are related phenomena (Rodionov et al, 2020).…”

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confidence: 99%

The application of Vavilov’s approaches to the phylogeny and evolution of cultivated species of the genus <i>Avena</i> L.

Loskutov,

Gnutikov,

Blinova

et al. 2023

Vestn. VOGiS

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The central problem that Vavilov was investigating was the overall concept of global plant genetic resources. The theoretical basis of this concept consisted of the law of homologous series in variation, research on the problem of species as a system, botanical and geographical bases of plant breeding, and the key theory of the centers of origin of cultivated plants. The VIR global collection of plant genetic resources collected by Vavilov and his associates from all over the world reflects the fullness of botanical, morphological and genetic diversity, and can be used for historical, evolutionary, phylogenetic and applied breeding research aimed at unlocking the potential of all the collection material. The whole diversity of cultivated oats, as was proved by Vavilov, had originated from segetal weeds. This process can be clearly traced in Spain on the example of the cultivated diploid species A. strigosa, A. abyssinica in Ethiopia, A. byzantina in Turkey and Iran, and on segetal forms of A. sativa. The studies of the morphological features as a whole do not yield a complete picture of the evolutionary and systematic status of some oat species and forms. The methods and approaches that use DNA markers and genomic technologies, and are promising for the study of oat polymorphism and phylogeny have been actively researched recently. A number of works devoted to the molecular aspects of the evolution and phylogeny of the genus Avena have recently appeared. The research uses various markers of genes, gene regions, intergenic spacers (internal and external), both nuclear and chloroplast and mitochondrial, genomic approaches and other modern methods. On the basis of a comprehensive study of the complete intraspecific diversity from different zones of the distribution range of cultivated oat species as well as on the basis of an analysis of data on the geography of forms and species distribution ranges, it was established that the process of hexaploid species formation also took place in the western part of the Mediterranean, and subsequently, when moving eastward, these forms started occupying all the vast spaces in the region of the Southwest Asian center, forming a large intraspecific diversity of wild forms and weedy ones in transit to cultivated hexaploid oat species. An analysis of the intraspecific diversity of landraces has specified the centers of morphogenesis of all cultivated oat species. The phylogenetic analysis of the representative intraspecific diversity of cultivated and wild Avena species carried out using next generation sequencing (NGS) showed that diploid species with A-genome variants are in fact not primary diploids, but a peculiar Mediterranean introgressive hybridization complex of species that sporadically enter into interspecific hybridization. It was established that the tetraploid cultivated species A. abyssinica had most likely originated from the wild A. vaviloviana. An analysis of the ways of A. sativa and A. byzantina domestication showed that the most widespread ribotype of the A. sativa hexaploid was inherited from A. ludoviciana, and the second most widespread one, from A. magna, while A. byzantina has two unique ribotype families, most likely inherited from an extinct oat species or a still undiscovered cryptospecies.

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The Origin and Resource Potential of Wild and Cultivated Species of the Genus of Oats (Avena L.)

et al. 2021

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Phenomenon of Multiple Mutations in the 35S rRNA Genes of the C Subgenome of Polyploid Avena L.

Cited by 8 publications

References 57 publications

Hybridization and diversity of aquatic macrophyte Sparganium L. (Typhaceae) as revealed by high-throughput nrDNA sequencing

Hybridization and diversity of aquatic macrophyte Sparganium L. (Typhaceae) as revealed by high-throughput nrDNA sequencing

The application of Vavilov’s approaches to the phylogeny and evolution of cultivated species of the genus <i>Avena</i> L.

The Origin and Resource Potential of Wild and Cultivated Species of the Genus of Oats (Avena L.)

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