We discuss R-parity violation (RPV) in semi-local and local F-theory constructions. We first present a detailed analysis of all possible combinations of RPV operators arising from semi-local F-theory spectral cover constructions, assuming an SU(5) GUT. We provide a classification of all possible allowed combinations of RPV operators originating from operators of the form 10 ·5 ·5, including the effect of U(1) fluxes with global restrictions. We then relax the global constraints and perform explicit computations of the bottom/tau and RPV Yukawa couplings, at an SO(12) local point of enhancement in the presence of general fluxes subject only to local flux restrictions. We compare our results to the experimental limits on each allowed RPV operator, and show that operators such as LLe c , LQd c and u c d c d c may be present separately within current bounds, possibly on the edge of observability, suggesting lepton number violation or neutron-antineutron oscillations could constrain F-theory models.