Phenols Removal from Hemicelluloses Pre-Hydrolysate by Laccase to Improve Butanol Production

Allard-Massicotte, Rosalie; Chadjaa, Hassan; Marinova, Mariya

doi:10.3390/fermentation3030031

Cited by 9 publications

(4 citation statements)

References 18 publications

(20 reference statements)

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“…The laccase from T. versicolor was reported to detoxify furanic and phenolic aldehyde derivatives, and in the presence of a redox mediator, this enzyme broadened its activity to phenolic ketone derivatives (Saravanakumar et al 2016). The butanol production by Clostridium acetobutylicum was 2.7-fold higher than the untreated wood hydrolysate (Allard-Massicotte et al 2017). According to the previous study, a combination of hydrolysate flocculation and laccase treatment reduced phenolic concentration from 1.20 to 0.28 g/L.…”

Section: Laccases (Aa1)mentioning

confidence: 79%

Enzymatic removal of inhibitory compounds from lignocellulosic hydrolysates for biomass to bioproducts applications

Tramontina

Brenelli

Sodré

et al. 2020

World J Microbiol Biotechnol

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The physicochemical pretreatment is an important step to reduce biomass recalcitrance and facilitate further processing of plant lignocellulose into bioproducts. This process results in soluble and insoluble biomass fractions, and both may contain by-products that inhibit enzymatic biocatalysts and microbial fermentation. These fermentation inhibitory compounds (ICs) are produced during the degradation of lignin and sugars, resulting in phenolic and furanic compounds, and carboxylic acids. Therefore, detoxification steps may be required to improve lignocellulose conversion by microoganisms. Several physical and chemical methods, such as neutralization, use of activated charcoal and organic solvents, have been developed and recommended for removal of ICs. However, biological processes, especially enzyme-based, have been shown to efficiently remove ICs with the advantage of minimizing environmental issues since they are biogenic catalysts and used in low quantities. This review focuses on describing several enzymatic approaches to promote detoxification of lignocellulosic hydrolysates and improve the performance of microbial fermentation for the generation of bioproducts. Novel strategies using classical carbohydrate active enzymes (CAZymes), such as laccases (AA1) and peroxidases (AA2), as well as more advanced strategies using prooxidant, antioxidant and detoxification enzymes (dubbed as PADs), i.e. superoxide dismutases, are discussed as perspectives in the field.

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Section: Laccases (Aa1)mentioning

confidence: 79%

Enzymatic removal of inhibitory compounds from lignocellulosic hydrolysates for biomass to bioproducts applications

Tramontina

Brenelli

Sodré

et al. 2020

World J Microbiol Biotechnol

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“…Instead, they found total inhibition of bioethanol production when working with a vanillin concentration of 4000 mg/L. In addition, Allard-Massicotte et al [ 91 ], in their study about phenols removal from pre-hydrolysate by laccase, reported that a phenolic compounds concentration of 280 mg/L was far below the limit of inhibition in the acetone-butanol-ethanol fermentation.…”

Section: Resultsmentioning

confidence: 99%

Liquid Hot Water Pretreatment and Enzymatic Hydrolysis as a Valorization Route of Italian Green Pepper Waste to Delivery Free Sugars

Martín-Lara

Chica-Redecillas

Pérez

et al. 2020

Foods

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In this work, liquid hot water pretreatment (autohydrolysis) was used to improve enzymatic hydrolysis of a commonly consumed vegetable waste in Spain, Italian green pepper, to finally produce fermentable sugars. Firstly, the effect of temperature and contact time on sugar recovery during pretreatment (in insoluble solid and liquid fraction) was studied in detail. Then, enzymatic hydrolysis using commercial cellulase was performed with the insoluble solid resulting from pretreatment. The objective was to compare results with and without pretreatment. The results showed that the pretreatment step was effective to facilitate the sugars release in enzymatic hydrolysis, increasing the global sugar yield. This was especially notable when pretreatment was carried out at 180 °C for 40 min for glucose yields. In these conditions a global glucose yield of 61.02% was obtained. In addition, very low concentrations of phenolic compounds (ranging from 69.12 to 82.24 mg/L) were found in the liquid fraction from enzymatic hydrolysis, decreasing the possibility of fermentation inhibition produced by these components. Results showed that Italian green pepper is an interesting feedstock to obtain free sugars and prevent the enormous quantity of this food waste discarded annually.

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“…In butanol production, the acetone‐ n ‐butanol‐ethanol fermentation process, phenolic compounds inhibit microorganisms such as Clostridium sp. A study by Allard‐Massicotte et al 75 used laccase, nanofiltration, and flocculation to reduce phenolic content from 1.20 to 0.28 g/L in wood prehydrolysate, allowing 4.17 g/L butanol production. In another study, Yuejiao et al 76 used the bacterial strain Kurthia huakuii to detoxify pretreated corn stover from phenolics with laccases produced by this strain and used this material to produce lactic acid with Bacillus coagulans in an SSF process, achieving titers up to 33.5 g/L compared to 18 g/L without the detoxification step.…”

Section: Applications Of Laccases In the Production Of Biofuels And B...mentioning

confidence: 99%

Removal of phenolic inhibitors from lignocellulose hydrolysates using laccases for the production of fuels and chemicals

Fernández‐Sandoval,

García,

Teymennet‐Ramírez

et al. 2023

Biotechnology Progress

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Lignocellulose is the most abundant biopolymer in the biosphere. It is inexpensive and therefore considered an attractive feedstock to produce biofuels and other biochemicals. Thermochemical and/or enzymatic pretreatment is used to release fermentable monomeric sugars. However, a variety of inhibitory by‐products such as weak acids, furans, and phenolics that inhibit cell growth and fermentation are also released. Phenolic compounds are among the most toxic components in lignocellulosic hydrolysates and slurries derived from lignin decomposition, affecting overall fermentation processes and production yields and productivity. Ligninolytic enzymes have been shown to lower inhibitor concentrations in these hydrolysates, thereby enhancing their fermentability into valuable products. Among them, laccases, which are capable of oxidizing lignin and a variety of phenolic compounds in an environmentally benign manner, have been used for biomass delignification and detoxification of lignocellulose hydrolysates with promising results. This review discusses the state of the art of different enzymatic approaches to hydrolysate detoxification. In particular, laccases are used in separate or in situ detoxification steps, namely in free enzyme processes or immobilized by cell surface display technology to improve the efficiency of the fermentative process and consequently the production of second‐generation biofuels and bio‐based chemicals.

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Phenols Removal from Hemicelluloses Pre-Hydrolysate by Laccase to Improve Butanol Production

Cited by 9 publications

References 18 publications

Enzymatic removal of inhibitory compounds from lignocellulosic hydrolysates for biomass to bioproducts applications

Enzymatic removal of inhibitory compounds from lignocellulosic hydrolysates for biomass to bioproducts applications

Liquid Hot Water Pretreatment and Enzymatic Hydrolysis as a Valorization Route of Italian Green Pepper Waste to Delivery Free Sugars

Removal of phenolic inhibitors from lignocellulose hydrolysates using laccases for the production of fuels and chemicals

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