Phenolics of suberized envelopes generated by isolated tomato locule protoplasts

Rao, Gonella S. R. L.; Willison, J. H. M.; Ratnayake, W. M. N.; Ackman, R. G.

doi:10.1016/s0031-9422(00)83140-4

Cited by 15 publications

(2 citation statements)

References 5 publications

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“…The fact that the anionic peroxidase is localized in the same region in which the suberin polymer is deposited argues strongly that the peroxidase is involved in the biosynthesis of this polymer and that this peroxidase is most probably involved in the polymerization of the phenolic components, to form the aromatic matrix of suberin. In addition to the earlier suggestions (22,26) many recent reports have demonstrated the presence of aromatic components in suberin enriched preparations from the periderm of wound-healed potato (7), Agave americana crystal idioblasts (12), green cotton fiber (31), the endodermis and hypodermis of corn roots (24), the roots of bean (28), and-the envelopes generated by tomato locule protoplasts (25). Furthermore, histochemical tests showed both lipid and phenolic components in hypodermal and endodermal cell walls of roots (23,27,29) which have been shown chemically and ultrastructurally to be suberized (20).…”

Section: Methodsmentioning

confidence: 79%

Immunocytochemical Localization and Time Course of Appearance of an Anionic Peroxidase Associated with Suberization in Wound-Healing Potato Tuber Tissue

Espelie¹,

Franceschi²,

Kolattukudy³

1986

Plant Physiol.

265

109

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Thin sections of wound-healing potato tuber tissue were stained with rabbit antibody prepared against a suberization-associated anionic peroxidase and then stained with a goat anti-rabbit antibody-fluorescein conjugate. When these sections were examined with an epiilluminating fluorescence microscope, bright green fluorescent linear deposits were observed on the inner side of cell walls in the periderm layer. Initial deposits which were often not contiguous throughout the wall were first observed in some cells after 3 days of wound-healing and subsequently these layers became more pronounced so that all 6 day old periderm cells had green fluorescent layers on their inner walls. This fluorescence was not present in the walls of parenchyma cells or in the walls of periderm cells treated with preimmune serum and anti-rabbit IgG-FITC conjugate.Thin sections of wound-healing potato tissue which were stained with anti-peroxidase antibody and a goat anti-rabbit antibody-rhodamine conjugate exhibited a similar time course of development with a bright reddish-orange fluorescent layer observed on the inside wall of periderm cells. The production of this suberization-associated anionic peroxidase in wound-healing tissue was also demonstrated by an immunobinding dot blot assay which showed that the largest increase in the enzyme level occurred between 4 and 6 days of wound-healing. The present results support the hypothesis that this anionic peroxidase is involved in the deposition of the aromatic polymeric domain of suberin.

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Section: Methodsmentioning

confidence: 79%

Immunocytochemical Localization and Time Course of Appearance of an Anionic Peroxidase Associated with Suberization in Wound-Healing Potato Tuber Tissue

Espelie¹,

Franceschi²,

Kolattukudy³

1986

Plant Physiol.

265

109

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“…Preparation of phenolic acetates. Phenolic acetates were made by treating the phenols (10 mgl with a mixture of acetic anhydride (1.5 ml), 3 N NaOH t0.5 ml) and crushed ice (5 g) for 5 min (5). The phenolic acetates were extracted with diethyl ether after acidifying with 3 N HC1.…”

Section: Gas Liquid Chromatography-mass Spectrometry Igc-ms)mentioning

confidence: 99%

Mass spectra of fatty acid derivatives, of isopropylidenes of novel glyceryl ethers of cod muscle and of phenolic acetates obtained with the finnigan mat ion trap detector

Ratnayake

Timmins

Ohshima

et al. 1986

Lipids

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The applicability of the Finnigan MAT Ion Trap Detector (ITD) mass spectrometer for structure determination in some selected fatty acids and their derivatives has been investigated. Isopropylidene derivatives of novel glyceryl ethers isolated from cod flesh and of phenolic acetates are included to indicate the potential for diverse structures and to clarify the protonation of ions. The ITD is a simple and unsophisticated gas liquid chromatograph-mass spectrometer, but the spectra obtained are in most respects comparable to those from more conventional electron impact mass spectrometers. However, due to the comparatively high background pressure (approximately 10(-3) torr) in the ionization chamber of the ITD, there is a tendency for both neutral and ionized molecules to acquire protons from other molecules or fragments through collision. In many cases, the molecular ion was observed as the protonated molecular ion (M + 1), as in chemical ionization mass spectrometry. These interactions can be minimized if the sample load is decreased. Phenolic acetates exhibit not only protonation of the molecular ion, but also protonation of stable fragmented neutral molecules or ions.

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Isoflavonoids

Dewick¹

1988

The Flavonoids

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Phenolics of suberized envelopes generated by isolated tomato locule protoplasts

Cited by 15 publications

References 5 publications

Immunocytochemical Localization and Time Course of Appearance of an Anionic Peroxidase Associated with Suberization in Wound-Healing Potato Tuber Tissue

Immunocytochemical Localization and Time Course of Appearance of an Anionic Peroxidase Associated with Suberization in Wound-Healing Potato Tuber Tissue

Mass spectra of fatty acid derivatives, of isopropylidenes of novel glyceryl ethers of cod muscle and of phenolic acetates obtained with the finnigan mat ion trap detector

Isoflavonoids

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