Phenolics of mycorrhizas and non-mycorrhizal roots of Norway spruce

Abstract

The occurrence and amount of soluble and insoluble phenolics in mycorrhizal and non-mycorrhizal roots of Picea abies (L.) Karst, were investigated, p-Hydroxybenzoic acid glucoside, picein, piceatannol and its glucoside, isorhapontin, catechin and ferulic acid could be identified by high-performance liquid chromatography in mycorrhizas of Picea abies-Lactarius deterrimus and Picea abies-Laccaria amethystea. Both types were collected from axenic cultures and the latter also from a spruce stand. The same phenolics occurred in non-mycorrhizal short roots from sterile cultures. However, the amounts of p-hydroxybenzoic acid glucoside, picein, catechin and cell wall-bound ferulic acid were considerably reduced in mycorrhizas from axenic culture, whereas the hydroxystilbenes piceatannol, its glucoside and worhapontin were not significantly reduced. Pure mycelia of Laccaria amethystea (Bull.) Murr, and Lactarius deterrimus Gröger were also analysed for phenolic compounds. Both fungal species contained none of the identified phenolics. The results are discussed with respect to mycorrhization in different mycorrhizal types.

In vitro Bioactivity and Antimicrobial Activity of Picea abies and Larix decidua Wood and Bark Extracts

“…These types of stilbenes aglycones and glucosides have previously been discovered in bark extracts (Pietarinen et al 2006). Moreover, piceoside, piceatannol and its glucoside, and isorhapontin have been detected in P. abies roots (Münzenberger et al 1990;Latva-Mäenpää et al 2014). However, various reports did not find stilbenes in the hydrophilic knotwood extractives of P. abies (Willför et al 2003).…”

“…Previously, five benzoic acid derivatives (syringic acids, p-hydroxybenzoic, vanillic, and anisic), two cinnamic acid derivatives, p-coumaric, and ferulic acids were identified in P. abies cells (Metsämuuronen and Siren 2014). Native ferulic acid and phydroxybenzoic acid glucoside were found in root extracts (Münzenberger et al 1990). Various stilbenes and stilbenes glucosides have been previously observed in different parts of P. abies (Metsämuuronen and Siren 2014).…”

“…"Extracts bioactivity," BioResources 11(4), 9421-9437. 9428in P. abies roots (Münzenberger et al 1990;Latva-Mäenpää et al 2014). However, various reports did not find stilbenes in the hydrophilic knotwood extractives of P. abies (Willför et al 2003).…”

Evaluation of Physical, Thermal and Spectroscopic Properties of Biofield Treated p-Hydroxyacetophenone

“…P-Hydroxyacetophenone (pHAP) is an aromatic ketone ( Figure 1) having wide commercial applications. It is also known as piceol that is a phenolic compound and can be obtained naturally from mycorrhizal roots of Norway spruces (Picea abies) [5,6]. It is used in the production of rubbers, plastics, agricultural chemicals, and pharmaceuticals.…”

Mycorrhizal colonization and phenolic compounds accumulation on roots of Eucalyptus dunnii maiden inoculated with ectomycorrhizal fungi

“…In incompatible pairings, phenolics accumulated in roots as a result of a hypersensitive reaction. In mycorrhizas of Picea abies, Larix decidua and Pinus sylvestris, a lower concentration of soluble and cell wall bound phenolics than in uninoculated roots was observed (Münzenberger et al, 1990(Münzenberger et al, , 1995(Münzenberger et al, , 1996. Later, they observed that laccase and peroxidase activities differed between mycorrhizas and uninoculated roots of P. abies and L. decidua (Münzenberger et al, 1997).…”