Phenolics and terpenoids change in response to yeast extract and chitosan elicitation in Zataria multiflora cell suspension culture

Bavi, Khadijeh; Khavari-Nejad, Ramazan Ali; Najafi, Farzaneh; Ghanati, Faezeh

doi:10.1007/s13205-022-03235-x

Cited by 11 publications

(5 citation statements)

References 34 publications

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“…When compared to catechin-producing tissue cultures of other species, a surprising conclusion can be drawn: Tilia calli are among the most efficient in vitro producers of catechin reported to date. In the calli of the current study, 1667-5937 µg g DW −1 catechin was detected, which is significantly higher than the catechin content of most cell suspension cultures and calli reported for a variety of genera [8,[44][45][46][47][48]. It must also be noted that the above studies mostly reported optimized production conditions.…”

Section: Catechin Derivatives From Organs and Tissue Cultures Of Tili...contrasting

confidence: 68%

Production of Polyphenolic Natural Products by Bract-Derived Tissue Cultures of Three Medicinal Tilia spp.: A Comparative Untargeted Metabolomics Study

Szűcs,

Cziáky,

Volánszki

et al. 2024

Plants

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Medicinal plant tissue cultures are potential sources of bioactive compounds. In this study, we report the chemical characterization of the callus cultures of three medicinal Tilia spp. (Tilia cordata, Tilia vulgaris and Tilia tomentosa), along with the comparison to bracts and flowers of the same species. Our aim was to show that calli of Tilia spp. are good alternatives to the calli of T. americana for the production of polyphenols and are better sources of a subset of polyphenolic metabolites, compared to the original organs. Calli were initiated from young bracts and grown on woody plant medium containing 1 mg L−1 2,4-D and 0.1 mg L−1 BAP. For chemical characterization, a quality-controlled untargeted metabolomics approach and the quantification of several bioactive compounds was performed with the use of LC-ESI-MS/MS. While bracts and flowers contained flavonoid glycosides (astragalin, isoquercitrin) as major polyphenols, calli of all species contained catechins, coumarins (fraxin, esculin and scopoletin) and flavane aglyca. T. tomentosa calli contained 5397 µg g DW−1 catechin, 201 µg g DW−1 esculin, 218 µg g DW−1 taxifolin and 273 µg g DW−1 eriodictyol, while calli from other species contained lower amounts. T. cordata and T. tomentosa flowers were rich in isoquercitrin, containing 8134 and 6385 µg g DW−1, respectively. The currently tested species contained many of the bioactive metabolites described from T. americana. The production of catechin was shown to be comparable to the most efficient tissue cultures reported. Flowers and bracts contained flavonoid glycosides, including tiliroside, resembling bioactive fractions of T. americana. In addition, untargeted metabolomics has shown fingerprint-like differences among species, highlighting possible chemotaxonomic and quality control applications, especially for bracts.

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Section: Catechin Derivatives From Organs and Tissue Cultures Of Tili...contrasting

confidence: 68%

Production of Polyphenolic Natural Products by Bract-Derived Tissue Cultures of Three Medicinal Tilia spp.: A Comparative Untargeted Metabolomics Study

Szűcs,

Cziáky,

Volánszki

et al. 2024

Plants

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“…Application of yeast extract to cell cultures of Trigonella foenum-graecum has also led to 1.66-fold increased content of diosgenin (İlgar et al 2021). In a recent report of Bavi et al (2022) where yeast extract at very high concentration (1200 mg/L) has been increased the contents of phenylpropanoids gallic acid, vanillin, salicylic acid, catechin, rutin and terpenoids carvacrol and thymol in cell suspension culture of Zataria multifora.…”

Section: Chitosanmentioning

confidence: 99%

Optimization of micropropagation protocol and elicitation of luteolin and rutin biomolecules using phenylalanine and chitosan in root callus of Rumex hastatus D. Don

Singh

Agrawal

2022

Plant Cell Tiss Organ Cult

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Rumex hastatus D. Don, a traditional medicinal plant known for its, antitumorous, antiangiogenic and antibacterial potential is continuously under threat of extinction due to over exploitation and climatic changes. Phytochemical analysis of different plant parts showed that roots had the maximum quantity of two bioactive molecules, luteolin and rutin and thus was selected for establishment of aseptic culture.In vitro technology has been employed for developing an e cient micropropagation protocols for its conservation, large scale production as well elicitation of the valuable bioactive compounds. Murashige and Skoog's medium supplemented with 5µM TDZ proved best for developing greenish-brown and compact calluses in 98% root cultures within 30 days. Such callus pieces on subculture to same but fresh medium differentiated an average of 10.50 ± 1.29 shoots which were elongated on MS basal medium. Such excised shoots, rooted in MS + 0.1 µM IBA medium with an average of 12.03 ± 2.49 roots per shoot.These plantlets were successfully acclimatized under greenhouse conditions and elds and their genetic delity was evaluated employing SRAP and SCoT markers which revealed their high similarity with mother plant proving their true-to-type nature. Interestingly, the in vitro regenerants showed elevated levels of phenols, avonoids, luteolin and rutin content and higher antioxidant activities over the mother plant as done by phosphomolybdenum and DPPH assays. For elicitation of bioactive compounds, 100 mg/L phenylalanine was best for enhancing optimum luteolin content (407.18 ± 10.58 µg/g d.w.) over control (22.10 ± 0.57 µg/g d.w.) almost, 17.42-fold, whereas for rutin, 25 mg/L chitosan proved most effective enhancing its synthesis up to 1169.07 ± 46.49 µg/g d.w. over control (312.41 ± 12.42 µg/g d.w.), i.e., 2.74-fold. To the best of our knowledge, this is our rst report on micropropagation and elicitation of luteolin and rutin in Rumex hastatus through root culture.

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“…Radjabian et al (2015) found that the exogenous application of salicylic acid (250 and 500 µM) to Salvia o cinalis and Salvia virgata shoots led to the upregulation of PAL genes and an increase in rosmarinic acid synthesis. In cell suspensions of Zataria multi ora exposed to 40 mg L − 1 , there was an increase in PAL activity and phenolic content (Bavi et al 2022). Meanwhile, Kamalipourazad et al (2016) reported a reduction in Scrophularia striata Boiss.…”

Section: Elicitor Effects On Volatile Compounds and Chemical Classesmentioning

confidence: 99%

Biochemical responses and volatile compounds in a peppermint chemotype grown in a controlled environment

Calgaro

Melisinas

Gonçalves

et al. 2023

Preprint

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Peppermint is a medicinal plant with great economic importance for its protective effects against biotic and abiotic factors. This study aimed to assess the vegetative growth, biochemistry and volatile production of peppermint plants under elicitation. Nodal segments were inoculated in flasks containing Murashige and Skoog medium with one of four treatments (50 µM salicylic acid, 200 mg L− 1 chitosan, 25 µM copper sulphate, and control) and maintained for 90 days in a controlled environment. Copper treatment increased shoot growth by 43% and 68% compared with salicylic acid and chitosan, respectively. Furthermore, copper elicitation reduced the oxidation rate to only 13% and produced plants with better architecture. Salicylic acid and chitosan treatments increased the total phenolic content by 38% and 40%, respectively, compared with the control. The ferric reducing assay showed that salicylic acid and chitosan treatments increased the plant’s antioxidant activity by 82% and 96%, respectively, compared with the control. However, β-carotene, flavonoids and anthocyanins decreased with these treatments. Phenylalanine ammonia-lyase activity increased by 63% and 54% in shoots elicited with salicylic acid and chitosan, respectively. Elicitor treatment increased the number of volatile compounds detected (control = 29, salicylic acid = 32, copper = 37, chitosan = 38). Elicitation promoted significant changes in plant metabolism and chemical composition, evidenced mainly by differences in the levels of 2-hydroxy-3-(3-methyl-2-butenyl)-3-cyclopenten-1-one, d-limonene, eucalyptol, caryophyllene and l-alanine ethylamide. Monoterpene hydrocarbons were the major class in control shoots, whereas oxygenated monoterpenes were the major compounds in elicited shoots.

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Phenolics and terpenoids change in response to yeast extract and chitosan elicitation in Zataria multiflora cell suspension culture

Cited by 11 publications

References 34 publications

Production of Polyphenolic Natural Products by Bract-Derived Tissue Cultures of Three Medicinal Tilia spp.: A Comparative Untargeted Metabolomics Study

Production of Polyphenolic Natural Products by Bract-Derived Tissue Cultures of Three Medicinal Tilia spp.: A Comparative Untargeted Metabolomics Study

Optimization of micropropagation protocol and elicitation of luteolin and rutin biomolecules using phenylalanine and chitosan in root callus of Rumex hastatus D. Don

Biochemical responses and volatile compounds in a peppermint chemotype grown in a controlled environment

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