Phenolic removal of olive-mill dry residues by laccase activity of white-rot fungi and its impact on tomato plant growth

Aranda, Elisabet; Sampedro, Inmaculada; Ocampo, J. A.; García-Romera, Inmaculada

doi:10.1016/j.ibiod.2006.06.006

Cited by 59 publications

(39 citation statements)

References 14 publications

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“…However, olive mill dry residue (DOR) contains phytotoxic components, mainly phenols, which are capable of inhibiting plant growth (Martín et al, 2002). Considerable efforts to decrease the phytotoxicity of DOR using biological, chemical and physical treatment methods have been made (Ginos et al, 2006;Aranda et al, 2006;Sampedro et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Reduced dry olive residue phytotoxicity in the field by the combination of physical and biological treatments

Aranda

Sampedro

García‐Sánchez

et al. 2012

J. Soil Sci. Plant Nutr.

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Olive oil extraction generates large amounts of olive mill residues (DOR) which may be used as organic fertilizer. The influence of a combination of physical fractionation and saprobe fungal incubation on the phytotoxicity of DOR was studied. The physical fractions of DOR, obtained following extraction using ethyl acetate (EDOR) and water (ADOR) were less phytotoxic than DOR with respect to the shoot dry weight of tomato. There was no relationship between the total phenol content of the different DOR physical fractions and their phytotoxicity. The saprophytic fungus Coriolopsis rigida reduced DOR and ADOR phytotoxicity and eliminated the phytotoxicity of EDOR. However, unlike the physical treatments of DOR, the decrease in the phenol content of EDOR and ADOR caused by C. rigida was closely paralleled to the decrease in their phytotoxicity. After 30 days of incubation, C. rigida was able to eliminate the phytotoxicity of EDOR on tomato plants grown in the field.

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Section: Introductionmentioning

confidence: 99%

Reduced dry olive residue phytotoxicity in the field by the combination of physical and biological treatments

Aranda

Sampedro

García‐Sánchez

et al. 2012

J. Soil Sci. Plant Nutr.

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“…DOR is phytotoxic when added in agronomic doses, although once proper fungal treatment is provided, phytotoxicity is eliminated (Aranda et al, 2006). The incubation of DOR with T. versicolor in a soy-support medium not only reduced its phytotoxicity but also appears to stimulate plant growth.…”

Section: Discussionmentioning

confidence: 99%

Integrated biovalorization of wine and olive mill by-products to produce enzymes of industrial interest and soil amendments

Reina¹,

Ullrich

García-Romera³

et al. 2016

Span. j. agric. res.

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An integral and affordable strategy for the simultaneous production of lignin-modifying and carbohydrate active enzymes and organic amendment, with the aid of a saprobe fungus was developed by using olive oil and wine extraction by-products. The polyporal fungus Trametes versicolor was cultivated in soy or barley media supplemented with dry olive mill residue (DOR) as well as with grape pomace and stalks (GPS) in solid state fermentation (SSF). This strategy led to a 4-fold increase in the activity of laccase, the principal enzyme produced by SFF, in DOR-soy media as compared to controls. T. versicolor managed to secrete lignin-modifying enzymes in GPS, although no stimulative effect was observed. GPS-barley media turned out to be the appropriate medium to elicit most of the carbohydrate active enzymes. The reuse of exhausted solid by-products as amendments after fermentation was also investigated. The water soluble compound polymerization profile of fermented residues was found to correlate with the effect of phytotoxic depletion. The incubation of DOR and GPS with T. versicolor not only reduced its phytotoxicity but also stimulated the plant growth. This study provides a basis for understanding the stimulation and repression of two groups of enzymes of industrial interest in the presence of different carbon and nitrogen sources from by-products, possible enzyme recovery and the final reuse as soil amendments.

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“…In fact T. versicolor and P. cinnabarinus reduced the phytotoxic effect of SDOR on shoot dry weight of tomato grown in non sterilized soil, whereas any of the saprophytic fungi were able to eliminate the phytotoxicity of ADOR. The phytotoxicity of dry olive mill residues has been attributed mainly to phenols and the decrease of phenols in olive residues by saprobe fungi has been observed Aranda et al, 2006). However, they differed in their ability to decrease the toxicity of both residues on tomato grown in soil.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that phytotoxicity of DOR can be reduced by incubating it with some inmobilized saprophytic fungi for 4 weeks . Also short period of incubation can reduce the toxic effect of water soluble substances in DOR on plants growth under hydroponic conditions (Aranda et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Dry matter and root colonization of plants by indigenous arbuscular mycorrhizal fungi with physical fractions of dry olive mill residue inoculated with saprophytic fungi

Aranda

Sampedro

Díaz

et al. 2010

Span. j. agric. res.

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We studied the influence of indigenous arbuscular mycorrhizal (AM) and saprobe fungi on the phytotoxicity of the physical fractions of dry olive mill residue (DOR). The physical extractions of DOR gave an aqueous (ADOR) and an exhausted (SDOR) fraction with less phytotoxicity for tomato than the original samples. The indigenous AM were able to decrease the phytotoxicity of SDOR inoculated with Trametes versicolor and Pycnoporus cinnabarinus on tomato. However, incubation of ADOR with both saprophytic fungi did not decrease its phytotoxicity in presence of the indigenous AM fungi. The percentage of root length colonized by indigenous AM strongly decreased in presence of DOR, around 80% of decrease at dose of 25 g kg -1 of DOR, but the level of mycorrhization was higher in presence of ADOR or SDOR (38% and 44% of decrease respectively at the same dose). There were no relationships between the effects of the physical fractions of DOR incubated with the saprobe fungi on AM colonization and on plant dry weight of tomato. Our results suggest that the phytotoxicity of the olive residues can be eliminated by the combination of physical extraction and by saprobe fungal inoculation and the use of this agrowaste as organic amendment in agricultural soil may be possible.Additional key words: biofertilizer, bioremediation, olive waste, phytotoxicity, saprobe fungi, Solanum lycopersicum L. ResumenMateria seca y colonización de raíces de plantas por hongos micorrícico arbusculares autóctonos en presencia de fracciones físicas de alpeorujo seco y extractado inoculado con hongos saprobios En este trabajo se ha estudiado la influencia de los hongos micorrízicos arbusculares autóctonos y hongos saprobios en la fitotoxicidad de las fracciones físicas del alpeorujo. La extracción física del alpeorujo con agua produjo un extracto acuoso (ADOR) y un sólido extractado (SDOR) con menor fitotoxicidad para las plantas de tomate que los controles con alpeorujo. Los hongos arbusculares autóctonos son capaces de disminuir la fitotoxicidad de SDOR inoculado con Trametes versicolor y Pycnoporus cinnabarinus en plantas de tomate. Sin embargo, la incubación del ADOR con dichos hongos no modificó la fitotoxicidad del residuo en plantas de tomate cultivadas en suelo no estéril. El porcentaje de longitud de raíz colonizada por los endófitos arbusculares autóctonos disminuyó drásticamente en presencia de alpeorujo (alrededor de un 80% de disminución a la dosis de 25 g kg -1 de DOR) aunque el nivel de micorrización fue superior en presencia de ADOR o SDOR (38 y 44% de disminución a la misma dosis). No se ha encontrado relación entre los efectos de las fracciones físicas del alpeorujo incubado con hongos saprobios en la colonización arbuscular y el peso seco de las plantas de tomate. Nuestros resultados indican que la fitotoxicidad de los residuos del olivar puede ser eliminada por el uso combinado de una extracción física y una incubación biológica con hongos saprobios; por tanto podemos considerar el uso de estos residuos como enmendantes or...

show abstract

Phenolic removal of olive-mill dry residues by laccase activity of white-rot fungi and its impact on tomato plant growth

Cited by 59 publications

References 14 publications

Reduced dry olive residue phytotoxicity in the field by the combination of physical and biological treatments

Reduced dry olive residue phytotoxicity in the field by the combination of physical and biological treatments

Integrated biovalorization of wine and olive mill by-products to produce enzymes of industrial interest and soil amendments

Dry matter and root colonization of plants by indigenous arbuscular mycorrhizal fungi with physical fractions of dry olive mill residue inoculated with saprophytic fungi

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