Phenolic profile of Douro wines and evaluation of their NO scavenging capacity in LPS-stimulated RAW 264.7 macrophages

Rebelo, Manuel J.; Sousa, Carla; Valentão, Patrı́cia; Rego, Rosa; Andrade, Paula B.

doi:10.1016/j.foodchem.2014.04.070

Cited by 18 publications

(8 citation statements)

References 29 publications

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“…In order to further confirm the increase of NO production, the content of iNOS in macrophages was measured by using ELISA. iNOS is the most important enzyme to catalyse NO after the activation of macrophages [24]. Consistent with the data of NO, the results collectively showed that OPL improved the level of iNOS, and the effect was significantly better than OP.…”

Section: Discussionsupporting

confidence: 82%

Activation of macrophages by the ophiopogon polysaccharide liposome from the root tuber of Ophiopogon japonicus

Sun

Meng

et al. 2016

International Journal of Biological Macromolecules

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Section: Discussionsupporting

confidence: 82%

Activation of macrophages by the ophiopogon polysaccharide liposome from the root tuber of Ophiopogon japonicus

Sun

Meng

et al. 2016

International Journal of Biological Macromolecules

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“…Cyanidin chloride exerted a bigger anti-inflammatory effect when tested in the prevention with co-administration assay (2.1 ± 0.3 µg/mL NO) reaching basal levels of NO production. As for red wines, Douro wines caftaric acid and malvidin-3-O-glucoside (59% of the determined compounds), were found to decrease NO production in LPS-induced RAW264.7 macrophages [24]. Anthocyanins, polyphenols composing Tannat grape skin, have already been reported for their anti-inflammatory effect by downregulating the expression of iNOS, COX-2, TNF-α, and IL-1β in murine BV2 microglial cells stimulated with LPS, as well as to inhibit LPS-induced pro-inflammatory context in the same type cells [25].…”

Section: Bioaccessibility Of Anti-inflammatory Compoundsmentioning

confidence: 94%

In Vitro Bioaccessibility of Extractable Compounds from Tannat Grape Skin Possessing Health Promoting Properties with Potential to Reduce the Risk of Diabetes

Fernández-Fernández

Iriondo‐DeHond

Nardin

et al. 2020

Foods

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Diabetes pathogenesis encompasses oxidative stress, inflammation, insulin malfunctioning and partial or total insulin secretion impairment, which leads to a constant hyperglycemia. Polyphenols are known to possess bioactive properties, being Tannat grape skin a natural and sustainable source of these compounds. The present study aimed to find out the bioaccessibility of health-promoting molecules composing a multifunctional extract from Tannat grape skin obtained under hydro-alcoholic-acid conditions. The identification of phenolic compounds in the samples was performed by ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Subsequently, the samples were in vitro digested mimicking the human oral gastrointestinal conditions and the bioactivity of the digest (antioxidant, anti-inflammatory and modulation of glucose metabolism) was assessed. Effect on glucose metabolism was estimated by measuring carbohydrases activity and the functionality of glucose transporters of small intestine cells in presence and absence of the digested extract. Flavonoids, phenolic acids and phenolic alcohols were the major phenol compounds detected in the extract. The bioaccessible compounds protected the intestinal cells and macrophages against the induced formation of reactive oxygen species (ROS) and nitric oxide (NO). In addition, glucose transporters were inhibited by the digested extract. In conclusion, the bioaccessible compounds of the extract, including phenols, modulated key biochemical events involved in the pathogenesis of diabetes such as oxidative stress, inflammation and glucose absorption. The extract was effective under prevention with co-administration conditions supporting its potential for either reducing the risk or treating this disease.

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“…Thus, the scavenging of NO or suppression of NO production by iNOS are clearly promising indices in the screening of new functional foods. 39,40 The studied bioactive formulations exhibited antibacterial activity against all bacteria tested ( Table 4). The P. ostreatoroseus basidioma ethanolic extract presented higher antibacterial activity than the correspondent mycelium extract against Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhimurium and Enterobacter cloacae.…”

mentioning

confidence: 99%

Bioactive formulations prepared from fruiting bodies and submerged culture mycelia of the Brazilian edible mushroom Pleurotus ostreatoroseus Singer

et al. 2015

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a Pleurotus ostreatoroseus is a Brazilian edible mushroom whose chemical characterization and bioactivity still remain underexplored. In this study, the hydrophilic and lipophilic compounds as well as the antioxidant, anti-inflammatory and antimicrobial activities of formulations (ethanol extracts) prepared with its fruiting bodies and submerged culture mycelia were compared. The bioactive formulations contain at least five free sugars, four organic acids, four phenolic compounds and two tocopherols. The fruiting body-based formulation revealed higher reducing power, DPPH scavenging activity, β-carotene bleaching inhibition and lipid peroxidation inhibition in brain homogenates than the mycelium-based preparation, as well as higher anti-inflammatory and antimicrobial activities. The absence of hepatotoxicity was confirmed in porcine liver primary cells. These functional responses can be related to the levels of bioactive components including phenolic acids, organic acids and tocopherols.

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Phenolic profile of Douro wines and evaluation of their NO scavenging capacity in LPS-stimulated RAW 264.7 macrophages

Cited by 18 publications

References 29 publications

Activation of macrophages by the ophiopogon polysaccharide liposome from the root tuber of Ophiopogon japonicus

Activation of macrophages by the ophiopogon polysaccharide liposome from the root tuber of Ophiopogon japonicus

In Vitro Bioaccessibility of Extractable Compounds from Tannat Grape Skin Possessing Health Promoting Properties with Potential to Reduce the Risk of Diabetes

Bioactive formulations prepared from fruiting bodies and submerged culture mycelia of the Brazilian edible mushroom Pleurotus ostreatoroseus Singer

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