Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae)

Simin, Nataša; Orčić, Dejan; Četojević-Simin, Dragana; Mimica–Dukić, Neda; Anačkov, Goran; Beara, Ivana; Mitić-Ćulafić, Dragana; Božin, Biljana

doi:10.1016/j.lwt.2013.05.023

Cited by 68 publications

(42 citation statements)

References 25 publications

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“…The analysis of hydroxyl radical-scavenging activity was carried out according to the method of Yanhong et al [5]. 0.1 ml of extract solution in methanol (varying concentration from 20 to 100 μg/ml) was mixed with 0.8 ml of reaction buffer (0.2 M KH2PO4/KOH buffer, pH 7.2, 1.6 μmol deoxyribose, 0.1 μmol iron ammonium sulphate and 0.1 μmol ethylenediaminetetraacetic acid (EDTA)).…”

Section: Hydroxyl Radical-scavenging Activitymentioning

confidence: 99%

“…Phenolic antioxidants have been claimed to have beneficial health functions for retarding aging and preventing cancer and cardiovascular diseases [5]. The interest in phenolic antioxidants has increased remarkably over the last decade because of their protective effects against different diseases, including cardiovascular, inflammatory and neurological diseases, as well as cancers.…”

Section: Introductionmentioning

confidence: 99%

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Hypolipidemic Action of hydroxycinnamic Acids from Cabbage (<i>Brassica oleracea L. var. capitata</i>) on Hypercholesterolaemic Rat in Relation to Its Antioxidant Activity

Ji¹,

Li²,

Gong³

et al. 2015

JFNR

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Cabbage extract containing chlorogenic acid has notable scavenging activity against hydroxyl radicals, superoxide anion radicals, DPPH radicals as well as potent reducing power. Oral administration of the extract (240 mg/kg BW/day) to hypercholesterolaemic rats reduced by high-fat diet for 30 days lowered their blood TC, TG, and LDL-C levels by 36.3, 43.5 and 45.8%, respectively. However, the blood HDL-C levels in the same treated rats increased by 17.1%. Treatment of hypercholesterolaemic rats with the extract significantly increased the GSH level along with enhanced SOD, CAT activities in liver tissues. Furthermore, the extract significantly decreased hepatic MDA as well as GPx and GR activities in extract-treated rats. It can therefore be concluded that the extract has a high hypolipidaemic activity and this may be attributed to its antioxidative potential.

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Section: Hydroxyl Radical-scavenging Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hypolipidemic Action of hydroxycinnamic Acids from Cabbage (<i>Brassica oleracea L. var. capitata</i>) on Hypercholesterolaemic Rat in Relation to Its Antioxidant Activity

Ji¹,

Li²,

Gong³

et al. 2015

JFNR

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“…Ekstrakt gajene vrste Allium cepa pokazao je značajno nižu in vitro antitumorsku aktivnost (IC 50 0,72 mg/ml) od samonikle vrste A. flavum. Ekstrakt vrste A. cepa pokazao je i nižu antiinflamatornu aktivnost u odnosu na A. flavum, dok im je antioksidativna aktivnost bila uporediva [15]. Navedene razlike u aktivnosti mogu se pripisati velikom broju biološki aktivnih jedinjenja koja se po pravilu nalaze kod samoniklih vrsta, što ih i čini izuzetno atraktivnim kandidatima za dobijanje novih lekova, nutraceutika i suplemenata u ishrani.…”

Section: Rezultati I Diskusijaunclassified

Non-tumor/tumor IC50 ratio as parameter for evaluation of in vitro antitumor activity of the Plantago, Allium and Tuber species

Četojević-Simin¹,

Beara²,

Simin³

2015

Lek sirovine

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2 Departman za hemiju, biohemiju i zaštitu životne sredine, Prirodno-matematički fakultet, Univerzitet u Novom Sadu, Trg Dositeja Obradovića 3, 21000 Novi Sad, Srbija IZVODCilj ovog rada bilo je uvođenje parametra za preciznu procenu efikasnosti in vitroantitumorske aktivnosti ekstrakata vrsta Plantago altissima, P. lanceolata, P. reniformis, Allium flavum, A. cepa, Tuber aestivum i T. magnatum. Za određivanje uticaja ekstrakata na rast ćelija korišćene su humane tumorske ćelijske linije HeLa, MCF7 i HT-29, kao i linija humanih zdravih ćelija MRC-5. Intenzitet ćelijskog rasta određen je merenjem ukupne količine proteina kolorimetrijskim sulforodamin B (SRB) testom. Prema NT/T IC 50 parametru najveću efikasnost u antitumorskom delovanju pokazali vodeni ekstrakti crnog i belog tartufa prema adenokarcinomu dojke sa izuzetno visokim vrednostima NT/T odnosa (NT/T=200-500). Efikasnost određenih matanolnih ekstrakata tartufa je bila visoka (NT/T=21,33-70): dok je efikasnost ekstrakata svih vrsta roda Plantago bila umerena (NT/T=3,24-4,27) prema adenokarcinomu dojke i karcinomu grlića materice. Najmanje efikasni bili su ekstrakti nadzemnog dela A. flavum ispoljili snažnije citotoksično delovanje prema zdravim ćelijama nego prema adenokarcinomu debelog creva (NT/T=0,23) i karcinomu cerviksa (NT/T=0,75). Veoma visoke NT/T vrednosti koje su dobijeni delovanjem vodenih ekstrakata crnog i belog tartufa preporučuju njihovu upotrebu u antitumorskoj terapiji, u proizvodnji nutraceutika i suplemenata u ishrani.Ključne reči: Antitumorska aktivnost, in vitro, prirodni proizvodi, Plantagosp., Allium sp., Tuber sp.

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“…They inhibit oxidising enzymes, chelation of transition metals, and transfer of hydrogen or single electron to radicals, and therefore act as cell saviours. 4,5 Physiological concentrations of free radicals are required to mediate physiological processes such as inflammatory reactions. Indeed, during inflammation, cells of the immune system are recruited to the site of damage.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo Antioxidant, Antihemolytic and Anti-inflammatory Activities of Santolina chamaecyparissus Extracts

Djarmouni¹,

Baghiani²,

Adjadj³

et al. 2018

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Introduction: Santolina chamaecyparissus L. is a small medicinal herb, cultivated in Europe, Asia and Africa due to the antihelmintic, antiseptic, anti spasmodic, bactericidal, fungicidal, digestive and vulnerary properties. Despite this, S. chamaecyparissus aerial part extractions have not been examined for antioxidant, antihemolytic and anti-inflammatory properties. Methods: S. chamaecyparissus aerial parts were extracted with solvents of varying polarity: methanol (crude) extract (CrE) chloroform extract (CHE), ethyl acetate extract (EAE), and aqueous extract (AE). The content of total phenolics, and flavonoids in all the extracts were determined with spectrophotometric methods. Both enzymatic and non-enzymatic methods were used to evaluate the antioxidant activity of the extracts. Antioxidant activity of all the extracts were investigated using free radical scavenging activity (1,1-diphenyl-2-picrylhydrazyl, DPPH• and 2,2'-azino-bis-3-ethyl benzthiazoline-6-sulfonic acid (ABTS) assay), capacity of the inhibition of linoleic acid peroxidation (β-carotene assay), chelation of metals (iron chelating assay) and inhibition of xanthine oxidase (XO) activity. To investigate antihemolytic activity of the extracts, the 2, 2,-azobis (2-amidinopropane) dihydrochloride (AAPH) assay was used to induce erythrocyte oxidative hemolysis. An in vivo approach was carried out on mice treated with the methanol extract at a dose of 100 mg/kg/day for 21 consecutive days, and one group was treated with vitamin C (vitamin C 50 mg/kg) as a standard drug. To determine the improvement of antioxidant potential, basic biochemical parameters were used in tissue (liver), plasma and whole blood. Phorbol 12-myristate 13-acetate (PMA)-induced ear edema was used to investigate the antiinflammatory activity of methanolic extract in mice. Results: Among all the extracts analyzed, the EAE exhibited a higher phenolic and flavonoids content (373.83 ± 0.23 mg gallic acid/g, and 61.51±7.86 mg quercetin/g respectively), followed by CE and CHE. DPPH and ABTS scavenging assays showed that EAE exhibited the highest effect in the both assays, with an IC 50 of 0.01 mg/ml and 0.0085 mg/ml respectively. All extracts moderately inhibited linoleic acid oxidation, with 57 % inhibition. The CE had a considerable chelating activity on ferrous iron (IC 50 = 0.32 mg/ml). In the enzymatic method by xanthine oxidase, results demonstrated that EAE had the highest XO inhibitory effect on both XO activity and Cyt-c reduction with IC50= 0.052±0.0003 mg/ml and 0.057±0.0006 mg/ml, respectively followed by CHE and CE. In the cellular system, CrE and CHE showed a hemolysis effect with % hemolysis (62.81% ± 1.43), (62.71% ± 1.01). However, EAE provided protection against AAPH-induced hemolysis with 53.67% ± 0.97. The in vivo assay exhibited a significant decrease (79.56%) of the content of malondialdehyde (MDA) in the liver and increased glutathione (GSH) and catalase (71.95% and 59.16% respectively). The methanol extract clearly demonstrated anti-inflammatory effects...

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Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae)

Cited by 68 publications

References 25 publications

Hypolipidemic Action of hydroxycinnamic Acids from Cabbage (<i>Brassica oleracea L. var. capitata</i>) on Hypercholesterolaemic Rat in Relation to Its Antioxidant Activity

Hypolipidemic Action of hydroxycinnamic Acids from Cabbage (<i>Brassica oleracea L. var. capitata</i>) on Hypercholesterolaemic Rat in Relation to Its Antioxidant Activity

Non-tumor/tumor IC50 ratio as parameter for evaluation of in vitro antitumor activity of the Plantago, Allium and Tuber species

In vitro and in vivo Antioxidant, Antihemolytic and Anti-inflammatory Activities of Santolina chamaecyparissus Extracts

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