Phenolic profile, antioxidant and anticandidal activities of Inula oculus-christi L. from Turkey

Abstract

The study aimed to determine the total phenol content of the extracts prepared with different solvents (70%methanol, ethyl acetate, 5% infusion) from the aerial parts of the I. oculus-christi, as well as to evaluate the antioxidant capacity and antimicrobial activity of the plant and to determine the main components responsible for the effect. The total phenolic content of the extracts prepared from the aerial parts of I. oculus-christi was calculated in gallic acid equivalents and the total phenolic content of the species was found to be in the range of 23.8-75.3 mg GAE/g. The highest total phenol content calculated as gallic acid equivalent was detected in the 5% infusion sample. The antioxidant activity of I. oculus-christi was also tested using DPPHˈ free radical scavenging in vitro assay (IC50: 0.092-1.03 mg/mL). The 5% infusion of the plant was shown to be the most effective extract in the antioxidant activity test performed using the DPPHˈ technique. The findings revealed that there is a correlation between total phenol content and antioxidant activity, as numerous research as suggested. By using the microdilution method, extracts from the aerial parts of I. oculus-christi were tested against Candida strains, and the MIC value for C. utilis was found to be 0.03 mg/mL in 5% infusion and ethyl acetate extracts. In terms of evaluating the biological effects obtained, the chemical composition of extracts was analyzed by HPLC-MS/MS, and the main components (5-caffeoylquinic acid, 1,5-dicaffeoylquinic acid, luteolin/kaempferol, apigenin, and rutin) were determined.

Gaillardin exerts potent antileukemic effects on HL‐60 cells and intensifies arsenic trioxide cytotoxicity: Providing new insight into sesquiterpene lactones in leukaemia treatment

“…Inula , a large genus of Asteraceae family, was a traditional remedy for jaundice, respiratory disorders, urinary tract infections and kidney stones 15 . As well as ethnopharmacological applications, some Inula species (e.g., Inula viscosa , Inula helenium , Inula Britannica and Inula japonica ) have been extensively studied for modern medical usage due to their remarkable healing nature 16 . The major secondary metabolites of Inula species are sesquiterpene lactones with potential antitumor activities, 17 and the α‐methylene‐γ‐lactone group of these compounds is responsible for their anticancer, anti‐inflammatory, antimicrobial and antioxidant effects.…”

“…15 As well as ethnopharmacological applications, some Inula species (e.g., Inula viscosa, Inula helenium, Inula Britannica and Inula japonica) have been extensively studied for modern medical usage due to their remarkable healing nature. 16 The major secondary metabolites of Inula species are sesquiterpene lactones with potential antitumor activities, 17 and the α-methylene-γ-lactone group of these compounds is responsible for their anticancer, anti-inflammatory, antimicrobial and antioxidant effects. Various types and positions of substituents result in the formation of distinct chemical structures.…”