Phenolic profile and antioxidant activity of bee pollen extracts from different regions of Algeria

Abstract

Due to its complex biochemical properties, the bee pollen is considered one of the functional foods. Bee pollen collected from pollen grains from different botanical sources offers almost a full diet such as carbohydrates, proteins, amino acids, vitamins, minerals. In this study, methanol extracts of 13 honeybee pollen samples were evaluated for flavonoids, phenolic compounds, and antioxidant capacity. Caffeic acid, chlorogenic acid, vanillic acid, p-coumaric acid, gallic acid, quercetin, rutin, vanillin, and naringin were identified as main phenolic compounds in pollen extracts by High-Performance Liquid Chromatography (HPLC) analysis. The obtained results are: total phenolic content - 379.8 to 915.6 mg GAE/100 g, total flavonoid content - 207.1 to 550 mg QE/100 g, and antioxidant activity - 808.2 to 3311 mg GAE/100 g in bee pollen extracts.

Determination of patulin in products containing dried fruits by Enzyme‐Linked Immunosorbent Assay technique Patulin in dried fruits

“…Studies have shown that 2 mg/ml propolis extract reduces the growth of PAT in apples juices (Silici & Karaman, 2014 ). Propolis is collected by honey bees, making it a rich source of aromatic acids, aromatic esters, volatile compounds, aromatic compounds, hydrocarbons, steroids, flavonoids, acids, micro‐ and macronutrients, vitamins, and essential oils (Hemmami et al., 2020 ; Pobiega et al., 2019 ). The abundance of bioactive compounds present in propolis or cinnamon oil which could reduce the expression of genes involved in PAT biosynthesis (Lai et al., 2021 ) suggests that the composition of herbal blends may also play a role in controlling optimal PAT growth conditions.…”

Analyzing the Antioxidant Activity and Fatty Acid Composition of Monofloral Mullein (Verbascum sp.) Pollen Oil obtained via Various Extraction Techniques

“…[31] Phenolic compounds were extracted using a solvent mixture consisting of ethanol and water. [25] However, alternative solvents like methanol, [32] water, [33] and a combination of water and acetone [34] were also utilized for this purpose.…”

Determination of some phenolic acids in Algerian propolis

“…After 3 min, 2 ml of Na2CO3 (20%) solution was added to the mixture. The reaction was held for 30 min in the dark, after which the absorbance was read at λ = 760 nm [26].…”

“…Regular operating standard solutions of phenolic acids are prepared by diluting the stock solution with the mobile step accordingly. Each of these solutions (20 μl) was injected into the column three times and reported peak area and retention periods [26].…”