Phenolic Fingerprint of Sicilian Modern Cultivars and Durum Wheat Landraces: A Tool to Assess Biodiversity

Bianco, Marisol Lo; Siracusa, Laura; Dattilo, Sandro; Venora, Gianfranco; Ruberto, Giuseppe

doi:10.1094/cchem-06-17-0125-r

Cited by 16 publications

(13 citation statements)

References 38 publications

(52 reference statements)

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“…× cornutum IPT211 and IPT214. The ability to identify and characterize local landraces by phenolic profile has been reported previously in different species, cultivars (Riggi et al, 2013; Lo Bianco et al, 2017), and local landraces of A. cepa (Riggi et al, 2013). In our study, the phenolic profile proved to be a powerful tool to discriminate among local shallot accession groups, especially with inclusion of minor phenolic compounds.…”

Section: Discussionmentioning

confidence: 66%

Morphological and Biochemical Diversity of Shallot Landraces Preserved Along the Croatian Coast

et al. 2018

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Shallots are a valuable minor Allium crop, and are propagated vegetatively and maintained in home gardens across generations along the Croatian coast and island areas. Shallot landraces growing along the Croatian coast fall into three genotypes: Allium cepa Aggregatum group (2n = 2x = 16), A. × proliferum (Moench) Schard. (2n = 2x = 16), and A. × cornutum Clementi ex Vis. (2n = 3x = 24), among which A. × cornutum is the most widespread. The aim of this study was to differentiate shallot accessions collected from local farmers using morphological markers. Also, the chemical composition including phenolic content, phenolic profile, total antioxidant capacity, and mineral composition, of shallot accessions was compared with that of the local landraces of common onion, and with market available shallot and common onion cultivars. Based on morphological observations and using multivariate classification, shallot landraces were classified into three distinct groups. Properties, based on which A. × cornutum can be differentiated from A. cepa Aggregatum and A. × proliferum, are stamen morphology, stamen length, leaf and scape vegetative properties, number of bulbs in cluster, cluster mass, and bulb diameter. Flower diameter and flower pedicel length differentiate A. × cornutum and A. × proliferum from A. cepa Aggregatum. Significant variability was observed in the biochemical profiles across tested accessions. Compared with the commercial common onion cultivars, local shallot accessions have higher bulb N, P, and K content. The major phenolic compounds identified in shallots were quercetin-4′-glucoside and quercetin-3,4′-diglucoside. Additionally, several other minor phenolic compounds were also identified. Morphological and biochemical profiles were evaluated using Partial Least Square (PLS) analysis. Specific morphological traits and biochemical markers for possible species identification are proposed.

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Section: Discussionmentioning

confidence: 66%

Morphological and Biochemical Diversity of Shallot Landraces Preserved Along the Croatian Coast

et al. 2018

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“…Nowadays attention is given to the cereals nutritional values and health effects and the terms “ancient grains”, “historical varieties”, “modern varieties” have become common in the scientific community and among consumers, covering a broad subject area, including the identification of varieties. Growing consumer interest in healthy food production has triggered interest in the phytochemical content of ancient and modern durum wheat varieties [10], and this trend led to the rediscovery and re-utilization of durum wheat landraces, including those in Sicily [11].…”

Section: Introductionmentioning

confidence: 99%

High-Throughput Genotype, Morphology, and Quality Traits Evaluation for the Assessment of Genetic Diversity of Wheat Landraces from Sicily

Fiore

Mercati

Spina³

et al. 2019

Plants

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During the XX Century, the widespread use of modern wheat cultivars drastically reduced the cultivation of ancient landraces, which nowadays are confined to niche cultivation areas. Several durum wheat landraces adapted to the extreme environments of the Mediterranean region, are still being cultivated in Sicily, Italy. Detailed knowledge of the genetic diversity of this germplasm could lay the basis for their efficient management in breeding programs, for a wide-range range of traits. The aim of the present study was to characterize a collection of durum wheat landraces from Sicily, using single nucleotide polymorphisms (SNP) markers, together with agro-morphological, phenological and quality-related traits. Two modern cv. Simeto, Claudio, and the hexaploid landrace, Cuccitta, were used as outgroups. Cluster analysis and Principal Coordinates Analysis (PCoA) allowed us to identify four main clusters across the analyzed germplasm, among which a cluster included only historical and modern varieties. Likewise, structure analysis was able to distinguish the ancient varieties from the others, grouping the entries in seven cryptic genetic clusters. Furthermore, a Principal Component Analysis (PCA) was able to separate the modern testers from the ancient germplasm. This approach was useful to classify and evaluate Sicilian ancient wheat germplasm, supporting their safeguard and providing a genetic fingerprint that is necessary for avoiding commercial frauds to sustaining the economic profits of farmers resorting to landraces cultivation.

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“…According to Lo Bianco et al [11], free phenolics were recovered by applying the method proposed by Dinelli et al [14] with few changes. In brief, 1 g of whole wheat flour was mixed under vigorous stirring for 10 min with 20 mL of an acidic aqueous methanol solution (80% methanol, 19% water, 1% formic acid).…”

Section: Extraction Of Free and Bound Phenolic Compoundsmentioning

confidence: 99%

“…According to Lo Bianco et al [11], three hydroxycinnamic acids were identified in wheat grains: caffeic acid (peak 4), ferulic acid (peak 10) and another member of this class (peak 1) for which unfortunately the MS spectrum was not determined. Vanillic acid (peak 3) was identified for its diagnostic UV-VIS and mass spectrum; the assignment was confirmed with co-injection with the corresponding standard.…”

Section: Phenolic Profile In Wheat Landracesmentioning

confidence: 99%

“…The rising price of these local productions are contributing to the farmers' satisfaction, changing an unprofitable job in a renewed professional opportunity also for young businessmen. Furthermore, many recent research studies testify the high healthy and nutraceutical value of landraces, both for high amount of antioxidant compounds and for their natural aptitude to organic production [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

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Phenolic Fingerprinting and Glumes Image Analysis as an Effective Approach for Durum Wheat Landraces Identification

Grillo¹,

Bianco²,

Blangiforti³

et al. 2018

Rediscovery of Landraces as a Resource for the Future

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In the last decade, encouraged by economic, social and nutritional reasons, the trend towards the rediscovery and reuse of durum wheat landraces moved on in Sicily. This growing attention in local wheat landraces made necessary to design new effective and objective identification methods that are able to distinguish landraces. Considering the difficulties coming from the genetic and morphological heterogeneity of a landrace, in this chapter a multidisciplinary approach for durum wheat landraces identification is proposed. Nine Sicilian wheat landraces were investigated from the genotypic and phenotypic point of view, studying their polyphenolic profile, and analyzing the glumes morpho-colorimetric traits, in search of similarities and/or differences. In particular, hydro-alcoholic extracts from whole wheat grains were analyzed by means of HPLC/ DAD and HPLC/ESI-MS, revealing 13 metabolites mainly belonging to the classes of hydroxycinnamic acids and flavones C-glycosides. The quantitative pattern of the 13 phenolic markers allowed to perfectly identify all the wheat samples, confirming a specific and genotype-dependent pattern of phenolics concentration. Moreover, computerized image analysis techniques were applied to compare the wheat samples on the basis of 138 quantitative morpho-colorimetric variables descriptive of glumes size, shape, color and texture, confirming the possibility to undoubtedly identify wheat samples belonging to local landraces.

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Phenolic Fingerprint of Sicilian Modern Cultivars and Durum Wheat Landraces: A Tool to Assess Biodiversity

Cited by 16 publications

References 38 publications

Morphological and Biochemical Diversity of Shallot Landraces Preserved Along the Croatian Coast

Morphological and Biochemical Diversity of Shallot Landraces Preserved Along the Croatian Coast

High-Throughput Genotype, Morphology, and Quality Traits Evaluation for the Assessment of Genetic Diversity of Wheat Landraces from Sicily

Phenolic Fingerprinting and Glumes Image Analysis as an Effective Approach for Durum Wheat Landraces Identification

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