Phenolic Contents and Antioxidant Potential of Crataegus Fruits Grown in Tunisia as Determined by DPPH, FRAP, and β‐Carotene/Linoleic Acid Assay

Abstract

Crataegusfruit is one of most important fruits in Tunisian flora. Some fruits of this genus are edible. This study was undertaken in order to examine the benefits of these fruits in human health and their composition of antioxidants including total polyphenol, flavonoids, proanthocyanidins content, and total anthocyanins. The antioxidative properties of the ultrasonic methanolic extract were assessed by different in vitro methods such as the FRAP, DPPH, andβ-carotene/linoleic acid assay. We concluded that peel fraction of red fruits possessed relatively high antioxidant activity and might be a rich source of natural antioxidants in comparison with the pulp and seed fruit extract. The results also showed that hawthorn yellow fruit presents lower amounts of phenolic content, absence of anthocyanins, and less antioxidant capacity. Most of peel and seed fractions were stronger than the pulp fractions in antioxidant activity based on their DPPH IC50, FRAP values, and results ofβ-carotene/linoleic acid. The total phenolic compounds contents were also highly correlated with the DPPH method and the FRAP assay.

Effect of Oven and Microwave Drying on Polyphenols Content and Antioxidant Capacity of Herbal Tea From Strobilanthes Crispus Leaves

“…Flavonoid compounds are considered to be the largest group of naturally occurring phenol and their antioxidant capacity are depending on the position of OH group [58]. Therefore, the antioxidant activity of plant extracts also comes from the presence of other antioxidant secondary metabolites, such as flavonoids, proanthocyanidins, anthocyanins, and not limited to phenolics content [59,60].…”

Total phenolics, antioxidant, antitumor, and enzyme inhibitory activity of Indian medicinal and aromatic plants extracted with different extraction methods

“…In b-carotene/linoleic acid assay, there is discoloration of b-carotene in reaction with linoleic acid-free radical which was generated upon the increased temperature due to the removal of hydrogen atoms coupled in two double bonds of linoleic acid (Amarowicz et al 2004). This reaction leads the loss of conjugation which automatically affects the decrease in absorbance at 470 nm for b-carotene, and thus the antioxidants present in solution protect reduction of b-carotene through reacting with other free radicals or linoleate-free radical in reaction system (Mraihi et al 2013). In this study, we found a considerable variation in the antioxidant capacity measured with DPPH, FRAP, and b-carotene/linoleic acid antioxidant assays using different extractions methods for medicinal plants selected from the Unani system of medicine (Table 3).…”

Bioactive compounds in aqueous infusions of dietary supplements and herbal blends containing dried hawthorn fruits or hawthorn inflorescences (Crataegus spp.)

“…C. monogyna fruits are characterized by about 2.0 times higher concentration of total phenolics than yellow fruits of C. azarolus and about 5.0 times higher concentration of total flavonols (Mraihi et al 2013, Mraihi et al 2015. Total amount of total phenolics in peel of C. monogyna fruits collected in Tunisia is between 45.70 ÷ 123.35 mg GAE 100g -1 DW (Mraihi et al 2013). Pliszka et al (2016) obtained higher concentration of TPC with use of exraction with citric acid were (913.0 ± 41.8 mg GAE 100 -1 FW) compared with extraction with 80% methanol (602.7 ± 17.0 mg GAE 100g -1 FW) for C. monogyna fruits.…”

“…In fresh HF of Crataegus monogyna total phenols was determined at level of 62.00 mg 100g -1 FW (Leja et al 2007). C. monogyna fruits are characterized by about 2.0 times higher concentration of total phenolics than yellow fruits of C. azarolus and about 5.0 times higher concentration of total flavonols (Mraihi et al 2013, Mraihi et al 2015. Total amount of total phenolics in peel of C. monogyna fruits collected in Tunisia is between 45.70 ÷ 123.35 mg GAE 100g -1 DW (Mraihi et al 2013).…”