Phenolic Content, HPLC Analysis and Antioxidant Activity Extract from <i>Tamarix gallica</i> and <i>Tamarix articulata</i> Growing in Southeast of Algeria

Said, Salemi; Gherraf, Noureddine; Eddine, Laouini Salah; Abdelmadjid, Guerram; Djamel, Berrani; Tliba, Ali

doi:10.5958/0974-360x.2018.00701.1

Cited by 15 publications

(10 citation statements)

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“…TA is a halophytic plant, growing in the extremely harsh environmental conditions. It exhibits promising biological activities such as antioxidant, antidiabetic, hypolipidemic, antibacterial, and hepatoprotective activities [15,16,18,[41][42][43]. TA displayed anti-tumor activity by modulating miRNA-1275 which plays a crucial role in the development of tumorigenesis [17].…”

Section: Discussionmentioning

confidence: 99%

“…One such plant in the deserts of Saudi Arabia containing a wide variety of secondary metabolites is TA [15]. This plant belongs to the family Tamaricaceae and is a halophytic plant growing in extremely harsh and arid conditions in the deserts of Saudi Arabia [16]. Commonly called Athal in the Arabic language, TA has been extensively used as a folk medicine by the Ta lalet, a tribal people in the south-eastern area of Morocco.…”

Section: Introductionmentioning

confidence: 99%

“…The plant is woody and possesses great medicinal value. Traditionally, the plant is used to treat various ailments such as gastrointestinal diseases, skin diseases, heart diseases, and other ailments [16].…”

Section: Introductionmentioning

confidence: 99%

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Tamarix Articulata Exhibits Antiproliferative and Antimetastatic Activity by Modulating PI3K-Akt and TGF-β-Mediated Downstream Signaling in Prostate Cancer Cells

Alnuqaydan

Almutary

Alajlan

et al. 2021

Preprint

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Anticancer drugs mainly kill tumor cells through the apoptosis mechanism, but they can become ineffective when tumor cells are metastatic. Thus, searching for plant-based extracts/compounds to curtail metastasis is extremely important. This study aims to evaluate the anticancer potential of Tamarix articulata (TA) extract against prostate cancer cells. MTT, Brd U, and trypan blue assays was performed to evaluate the cell viability. TUNEL assay were performed to determine apoptotic cells. Clonogenic, wound healing and Boyden chamber assay were conducted to evaluate the anti-clonogenic, anti-motility, and anti-invasive potential of TA. Zymography and immunoblotting were done to check the activity and expression of metalloproteases and proteins associated with metastasis. Our results demonstrated that TA extract significantly inhibits cell viability, clonogenic property, and displays IC₅₀ values in the 245–289 µg/mL range. TA extract significantly abrogates the motility and invasive property of LnCaP cells in a dose-dependent manner. Mechanistically, TA extract downregulates the expression of PI3K-Akt/TGF-β-SMAD2/3 and MMP-2/-9 with concomitant upregulation of TIMP1 expression in LnCaP cells. Additionally, we observe a dose-dependent downregulation of snail and vimentin with the upregulation of E-cadherin protein expression in LnCaP cells. In conclusions, TA extract exhibits an antiproliferative effect, abrogates cell motility and invasion by downregulating PI3K-Akt/TGF-β-SMAD2/3, MMP-2/9, snail, and vimentin with concomitant upregulation of E-cadherin and TIMP1 expression in prostate cancer cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tamarix Articulata Exhibits Antiproliferative and Antimetastatic Activity by Modulating PI3K-Akt and TGF-β-Mediated Downstream Signaling in Prostate Cancer Cells

Alnuqaydan

Almutary

Alajlan

et al. 2021

Preprint

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“…One such plant in the deserts of Saudi Arabia containing a wide variety of secondary metabolites is Tamarix articulata (TA) (Alnuqaydan and Rah, 2019). This plant belongs to the family Tamaricaceae and is a halophytic plant growing in extremely harsh and arid conditions in the deserts of Saudi Arabia (Said et al, 2018). Commonly called Athal in the Arabic language, TA has been extensively used as folk medicine by the Tafilalet, a tribal people in the southeastern area of Morocco.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the cytotoxic activity of Tamarix articulata and its anticancer potential in prostate cancer cells

M.¹,

Almutary²,

Y.³

et al. 2022

J App Pharm Sci

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Anticancer drugs induce cell death mechanism in tumor cells by various modes, which include apoptosis, autophagy, and necroptosis. However, they become ineffective when tumor cells enter metastasis. Thus, searching for plantbased extracts/compounds to curtail metastasis is extremely important. This study aims to evaluate the anticancer potential of Tamarix articulata (TA) extract against prostate cancer cells. MTT, Brd U, and trypan blue assays were carried out to evaluate the cell viability. Terminal Transferase dUTP Nick End Labeling assay was carried out to determine apoptotic cells. Clonogenic, wound-healing, and Boyden chamber assay was conducted to evaluate the anticlonogenic, antimotility, and anti-invasive potential of TA. Zymography and immunoblotting were carried out to check the activity and expression of metalloproteases and proteins associated with metastasis. Our results demonstrated that TA extract significantly inhibits cell viability and clonogenic property and displays IC 50 values in the 245-289 µg/ml range. TA extract significantly abrogates the motility and invasive property of LnCaP cells in a dose-dependent manner. Mechanistically, TA extracts downregulate the expression of phosphoinositide 3-kinaseprotein kinase B-protein kinase B (PI3K-Akt), transforming growth factor-beta-Sma genes and the Drosophila Mad, mothers against decapentaplegic protein 2/3 (TGF-β-SMAD2/3), and matrix metalloproteinases-2/9 (MMP-2/9) with concomitant upregulation of tissue inhibitor of metalloproteinase 1 (TIMP1) expression in LnCaP cells. Additionally, we observed a dose-dependent downregulation of snail and vimentin with the upregulation of E-cadherin protein expression in LnCaP cells. TA extract exhibits an antiproliferative effect and abrogates cell motility and invasion by downregulating PI3K-Akt, TGF-β-SMAD2/3, MMP-2/9, snail, and vimentin with concomitant upregulation of E-cadherin and TIMP1 expression in prostate cancer cells.

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“…(16). Traditionally, the plant is used to treat various ailments such as gastrointestinal diseases, skin diseases heart diseases, and other ailments (17). The major chemical constituents of TA extract of dry leaves are quinic acid, gallic acid, kaempferol, quercetin, Tamarixetin, epucatechin gallate and epiafzelechin.…”

Section: Introductionmentioning

confidence: 99%

Tamarix articulata Inhibits Cell Proliferation, Promotes Cell Death Mechanisms and Triggers G0/G1 Cell Cycle Arrest in Hepatocellular Carcinoma Cells

Alnuqaydan

2021

Food Technol. Biotechnol. (Online)

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Research background. From ancient times plants have been used for medicinal purposes against various ailments. In the modern era, plants are a major source of drugs and are an appealing drug candidate for the anticancer therapeutics against various molecular targets. Here we tested Tamarix articulata methanolic extract of dry leaves for anticancer activity against a panel of hepatocellular carcinoma cells. Experimental approach. Cell viability of hepatocellular carcinoma cells was determined by MTT assay after dose-dependent treatment with extract of Tamarix articulata. Phase-contrast microscopy and DAPI staining were performed to analyze cellular and nuclear morphology. Immunoblotting was performed to determine the expression of proteins associated with autophagy, apoptosis, and cell cycle. However, flow cytometry was used for the quantification of apoptotic cells and the analysis of cells in different phases of the cell cycle after treatment with varying doses of Tamarix articulata. Additionally, acridine orange staining and DCFHDA dye were used to analyze the quantification of autophagosomes and reactive oxygen species. Results and conclusion. Our results demonstrate that Tamarix articulata methanolic extract exhibits promising antiproliferative activity with IC50 values (271±4.38), (298±7.08) and (336±6.11) µg/mL against HepG2, Huh7D12, and Hep3B cell lines, respectively. Mechanistically, we found Tamarix articulata methanolic extract induces cell death by activating apoptosis and autophagy pathways. First, Tamarix articulata methanolic extract promotes autophagy which was confirmed by acridine orange staining. The immunoblotting analysis further confirms that Tamarix articulata methanolic extract consistently induces the conversion of, LC3I to LC3II form with a gradual decrease in expression of autophagy substrate protein p62 at higher doses. Second, Tamarix articulata methanolic extract promotes reactive oxygen species production in hepatocellular carcinoma cells and activates reactive oxygen species-mediated apoptosis. Flow cytometry and immunoblotting analysis showed that Tamarix articulata methanolic extract induces dose-dependent apoptosis and activates proapoptotic proteins caspase-3 and PARP1. Additionally, Tamarix articulata methanolic extract triggers the arrest of the G0/G1 phase of the cell cycle and upregulates the protein expression of p27/Kip, and p21/Cip, with a decrease in cyclin-D1 expression in hepatocellular carcinoma cells. Novelty and scientific contribution. The current study demonstrates that Tamarix articulata methanolic extract exhibits promising anticancer potential to kill tumor cells by programmed-cell-death type I and II mechanisms and could be explored for potential drug candidate molecules to curtail cancer in the future.

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Phenolic Content, HPLC Analysis and Antioxidant Activity Extract from Tamarix gallica and Tamarix articulata Growing in Southeast of Algeria

Cited by 15 publications

References 0 publications

Tamarix Articulata Exhibits Antiproliferative and Antimetastatic Activity by Modulating PI3K-Akt and TGF-β-Mediated Downstream Signaling in Prostate Cancer Cells

Tamarix Articulata Exhibits Antiproliferative and Antimetastatic Activity by Modulating PI3K-Akt and TGF-β-Mediated Downstream Signaling in Prostate Cancer Cells

Evaluation of the cytotoxic activity of Tamarix articulata and its anticancer potential in prostate cancer cells

Tamarix articulata Inhibits Cell Proliferation, Promotes Cell Death Mechanisms and Triggers G0/G1 Cell Cycle Arrest in Hepatocellular Carcinoma Cells

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