Phenolic compounds protect HepG2 cells from oxidative damage: Relevance of glutathione levels

Lima, Cristóvão F.; Fernandes-Ferreira, Manuel; Pereira‐Wilson, Cristina

doi:10.1016/j.lfs.2006.06.042

Cited by 204 publications

(183 citation statements)

References 87 publications

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“…The difference in protective ability between HA and HAPa might be caused by the difference in the peptides. Lima et al (2006) noted that the antioxidant efficacy on cell depends on both ability to penetrate the cell membrane and the antioxidant capacity in solution. Due to a higher DH obtained in HAPa, low MW peptides plausibly penetrated into lipid bilayer of HepG2 cell more effectively and reacted with free radical inside cells.…”

Section: Effect Of Oxidative Stressors On Cytotoxicity Of Hepg2 Cellmentioning

confidence: 99%

Preventive effect of Nile tilapia hydrolysate against oxidative damage of HepG2 cells and DNA mediated by H2O2 and AAPH

et al. 2015

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Antioxidant activities of protein hydrolysate prepared from Nile tilapia protein isolate using Alcalase (HA), Alcalase followed by papain (HAPa) and their Sephadex G-25 fractions (FHA and FHAPa) were investigated in both chemical and cellular based models. Amongst all samples, FHAPa showed the highest chemical antioxidant activities, however it had no metal chelation activity. Cellular antioxidant ability of HA, HAPa and their fractions against H 2 O 2 and AAPH induced oxidative damage of HepG2 cell and DNA were tested. When cells were pretreated with all hydrolysates or fractions at different concentrations (0.5-2 mg/mL) in the absence and presence of 50 μM Trolox, cell viability was in the range of 91.10-111.40 %. However, no difference in cell viability was observed among samples having various concentrations (P>0.05). Cell reactive oxygen species (ROS) generation as mediated by H 2 O 2 and AAPH decreased with treatment of hydrolysates or their fractions, especially in combination with 50 μM Trolox. FHAPa effectively inhibited H 2 O 2 and peroxyl radical induced DNA scission in a dose dependent manner. Therefore, Nile tilapia protein hydrolysates could serve as a functional food ingredient.

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Section: Effect Of Oxidative Stressors On Cytotoxicity Of Hepg2 Cellmentioning

confidence: 99%

Preventive effect of Nile tilapia hydrolysate against oxidative damage of HepG2 cells and DNA mediated by H2O2 and AAPH

et al. 2015

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“…This method described previously by Lima et al (2006). The samples were reacted with the stable DPPH radical in a methanol solution.…”

Section: Reducing Powermentioning

confidence: 99%

Antioxidant activity of raspberry (Rubus fruticosus) leaves extract and its effect on oxidative stability of sunflower oil

2014

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Efficacy of R. fruticosus leaves extract in stabilizing sunflower oil during accelerated storage has been studied. Extracts of R. fruticosus were prepared in different solvents which methanolic extract yield with 15.43 % was higher than water and acetone ones (11.87 and 6.62 %, respectively). Methanolic extract was chosen to evaluate its thermal stability at 70°C in sunflower oil, due to the highest yield, antioxidant and antiradical potential and also high content of phenolic compounds campared to other solvents. So, different concentrations of methanolic extract (200, 400, 600, 800 and 1,000 ppm) were added to sunflower oil. BHA and BHT at 200 ppm served as standards besides the control. Peroxide value (PV) and thiobarbituric acid (TBA) were taken as parameters for evaluation of effectiveness of R. fruticosus leaves extract in stabilization of sunflower oil. Moreover, antioxidant activity index (AAI) of the extract at 120°C at rancimat were conducted. Results from different parameters were in agreement with each other, suggesting the highest efficiency of 1,000 ppm of the extract followed by BHT, BHA and other concentrations of the extract. Results reveal the R. fruticosus leaves extract to be a potent antioxidant for stabilization of sunflower oil.

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“…1B). Because t-BHP is known to induce DNA damage, [17][18][19] we assessed DNA condensation using DAPI staining. Treatment with t-BHP for 24 h caused approximately 70% of cells to show condensed DNA (Fig.…”

Section: Hi Protects Human Fibroblasts Against T-bhp-induced Oxidationmentioning

confidence: 99%

The Anti-apoptotic Action of 5-Hydroxyindole: Protection of Mitochondrial Integrity

Bae

Lee

et al. 2010

Biological & Pharmaceutical Bulletin

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5-Hydroxyindole (5HI) is a metabolite of tryptophan that is involved in learning and memory as well as in central nervous system regulation, such as in blocking desensitization.1) 5HI also has anti-oxidant activity, 2,3) but its cellular targets are unclear.Oxidative stress can be induced by endogenous processes in which the balance between reductants and oxidants becomes slanted towards the oxidative side. Most oxidants are derived from oxygen and nitrogen based reactive species (RS), including reactive oxygen species (ROS) and lipid peroxidation byproducts.4) ROS cause oxidative modification and play important roles in abnormal pathological processes and biochemical functions. 5,6) ROS also can induce apoptosis in many different cell systems. 7) tert-Butylhydroperoxide (t-BHP) can cause oxidative stress and cell damage. 8) t-BHP is a strong RS inducer of lipid peroxidation, and causes glutathione (GSH) depletion and peroxynitrite (ONOO Ϫ ) generation.9,10) A short-chain analog of lipid hydroperoxides, t-BHP is used often as a model compound to establish the mechanism of cell damage initiated by oxidative stress. 8)Mitochondrial integrity is constantly threatened by the presence of RS produced in the membrane. High RS levels in the mitochondria can compromise mitochondrial membrane potential (Dym), leading to depolarization 11) and apoptotic events, including cytochrome c release and caspase activation.12) Here, we attempted to identify the cellular mechanisms of 5HI by focusing on mitochondria and associated apoptotic processes. Our findings show that 5HI treatment inhibited damage from oxidative stress and apoptosis in t-BHP-induced human fibroblast cell. These findings are supported by the data gained on GSH, lipid peroxidation, RS generation, DNA damage, decreased mitochondrial membrane potential, cytochrome c release, and caspase activity. MATERIALS AND METHODS MaterialsHuman fibroblasts were obtained from the American Type Culture Collection (ATCC, Manassas, VA, U.S.A.). 5HI and Dulbecco's modified Eagle's media (DMEM) were purchased form Sigma-Aldrich Chemical (St. Louis, MO, U.S.A.), as were all reagents unless otherwise stated. Fetal bovine serum (FBS), trypsin-ethylenediamineteraacetic acid, and penicillin-streptomycin were purchased from Gibco BRL (Ground Island, NY, U.S.A.). 3-(4,5-Dimethylthiazol-yl)-diphenyl tetrazolium bromide (MTT) was provided by DUCHEFA Biochemie (Haarlem, Netherlands). Antibodies for human cytochrome c, cleavedcaspase-3, and caspase-9 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, U.S.A.). Human a-tubulin antibody and 2Ј,7Ј-dichlorodihydrofluorescein diacetate (DCFDA) were obtained from Sigma-Aldrich (St. Louis, MO, U.S.A.) Horseradish peroxidase-conjugated goat antirabbit immunoglobulin G (IgG) and goat anti-mouse IgG was provided by Santa Cruz (Santa Cruz, CA, U.S.A.). 5HI was dissolved in dimethyl sulfoxide (DMSO), and the final concentration of DMSO was Ͻ0.1%.Cell Culture and MTT Assay Human fibroblast cells were cultured in DMEM containing 10% FBS, 2 mM gl...

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Phenolic compounds protect HepG2 cells from oxidative damage: Relevance of glutathione levels

Cited by 204 publications

References 87 publications

Preventive effect of Nile tilapia hydrolysate against oxidative damage of HepG2 cells and DNA mediated by H2O2 and AAPH

Preventive effect of Nile tilapia hydrolysate against oxidative damage of HepG2 cells and DNA mediated by H2O2 and AAPH

Antioxidant activity of raspberry (Rubus fruticosus) leaves extract and its effect on oxidative stability of sunflower oil

The Anti-apoptotic Action of 5-Hydroxyindole: Protection of Mitochondrial Integrity

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